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Differences in the contributions of dietary water to the hydrogen stable isotope ratios of cultured Atlantic salmon and Arctic charr tissues

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Abstract

Hydrogen stable isotopes of animal tissues are well established tracers of migration ecology in terrestrial ecosystems. Recent research has highlighted δ2H as a potential tool in studies of aquatic ecosystems, particularly as a robust tracer for quantifying the importance of allochthonous subsidies. Although the use of δ2H has clear potential, some uncertainties remain, in particular with regard to the contribution of dietary water to consumer δ2H. Here, we quantify the contribution of dietary water to δ2H in two salmonid fishes, Atlantic salmon (Salmo salar L.) and Arctic charr (Salvelinus alpinus L.), reared on diets of known isotopic composition. Furthermore, we examined the capacity of fins (adipose and caudal) to provide a non-lethal means of estimating consumer δ2H. The proportion of deuterium derived from environmental water of all tissue was substantial in both Atlantic salmon (mean = 0.43 ± 0.1 SD) and Arctic charr (mean = 0.48 ± 0.15 SD) but varied considerably between both individuals and tissue type. White muscle proved to be the least variable of the tissues analysed. Although fins proved to be a possible non-destructive substitute, a degree of caution is recommended with their use, as the proportion of dietary water contributing to the deuterium of fins was considerable more variable.

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Acknowledgments

This work was funded through a grant awarded by Science Foundation Ireland (05/RFP/EEB0055) under the Research Frontier Programme. We would like to thank P. McGovern of Stofinfiskur for use of facilities and R. Doucett of Colarado Pleateau Stable Isotope Laboratory for advice on stable isotopes of hydrogen.

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Authors and Affiliations

  1. School of Biological, Earth and Environmental Sciences, University College Cork, Enterprise Centre, Distillery Fields, North Mall, Cork, Ireland

    C. T. Graham & S. S. C. Harrison

  2. School of Biological Sciences, Medical Biology Centre, Queen’s University Belfast, 97 Lisburn Road, Belfast, BT9 7BL, UK

    C. Harrod

  3. Instituto de Investigaciones Oceanológicas, Universidad de Antofagasta, Avenida Angamos 601, Antofagasta, Chile

    C. Harrod

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  1. C. T. Graham
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  2. S. S. C. Harrison
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Correspondence to C. T. Graham.

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Graham, C.T., Harrison, S.S.C. & Harrod, C. Differences in the contributions of dietary water to the hydrogen stable isotope ratios of cultured Atlantic salmon and Arctic charr tissues. Hydrobiologia 721, 45–55 (2014). https://doi.org/10.1007/s10750-013-1636-0

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