Skip to main content
SpringerLink
Log in

Investigation of genome polymorphism and seed coat anatomy of species of section Adenolinum from the genus Linum

  • Research Article
  • Published:
Genetic Resources and Crop Evolution Aims and scope Submit manuscript

Abstract

Twelve species of section Adenolinum from genus Linum have been studied using cytogenetic methods (DAPI/C-banding, Ag-NOR staining, FISH with 5S and 26S rDNA probes), RAPD analysis, and seed surface ultrastructure and seed coat anatomy investigation. Karyotype analysis revealed that these species have a chromosome number of 2n = 18 or 2n = 36. The DAPI/C-banding patterns enabled the identification of all chromosomes and revealed the similarities in chromosome patterns in all of the studied species. It was found that 18-chromosome plants are diploids, whereas 36-chromosome forms are tetraploids. In diploid species, 5S and 26S rRNA genes are located in similar positions on a single pair of chromosomes or on two chromosome pairs in tetraploids. Ag-NOR staining showed NOR activity in both genomes of tetraploids. RAPD analysis and seed coat anatomy investigation revealed some interspecies differences. Species from nearby areas have more similar RAPD characteristics compared with more geographically distant species. The results of this study may serve as an additional argument in favour of a monophyletic origination of the Adenolinum species and its strong isolation from other Linum species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Adams SP, Leitch IJ, Bennett MD, Chase MW, Leitch AR (2000) Ribosomal DNA evolution and phylogeny in Aloe (Asphodelaceae). Am J Bot 87(11):1578–1583

    Article  PubMed  CAS  Google Scholar 

  • Alefeld F (1867) Ueber Adenolinum Rchb. Bot Zeit Bd 25:249–255

  • Belyaev AA (2009) The role of mobile elements in plant microevolution by example of Aegilops speltoides (Triticeae, Poaceae): molecular cytogenetic and molecular genetic analysis. PhD Thesis, St. Petersburg State University, St. Petersburg

  • Belyaev A, Raskina O, Nevo E (2001) Evolutionary dynamics and chromosomal distribution of repetitive sequences on chromosomes of Aegilops speltoides revealed by genomic in situ hybridization. Heredity 86:738–742

    Article  Google Scholar 

  • Bougourd SM, Parker JS (1976) Nucleolar-organiser polymorphism in natural populations of Allium schoenoprasum. Chromosoma 56:301–307

    Article  Google Scholar 

  • Chennaveeraiah MS, Joshi KK (1983) Karyotypes in cultivated and wild species of Linum. Cytologia 48:833–841

    Article  Google Scholar 

  • Cullis CA (2005) Mechanisms and control of rapid genomic changes in flax. Ann Bot 95:201–206

    Article  PubMed  CAS  Google Scholar 

  • Cullis CA (2007) Flax. In: Kole C (ed) Genome mapping and molecularbreeding in plants, vol.2 Oilseeds. Springer, Berlin, Heidelberg, pp 275–295

  • Dong F, Mille JT, Jackson SA, Wang GL, Ronal P, Jiang J (1998) Rice (Oryza sativa) centromeric regions consist of complex DNA. Proc Natl Acad Sci USA 95:8135–8140

    Article  PubMed  CAS  Google Scholar 

  • Egorova TV (1996) Genus Linum—Linaceae DC. ex S.F.Gray. In: Tsvelev NN (ed) Flora Vostochnoi Evropy (East European Flora), vol 9. St. Petersburg Publishing House “Mir i Semia”, pp 347–360

  • Franz PF, Armstrong S, de Jong H, Parnell LD, Drunen CV, Dean C, Zabel P, Bisseling T, Jones G (2000) Integrated cytogenetic map of chromosome arm 4S of A. thaliana: structural organization of heterochromatic knob and centromere region. Cell 100:367–376

    Article  Google Scholar 

  • Fu YB, Allaby RG (2010) Phylogenetic network of Linum species as revealed by non-coding chloroplast DNA sequences. Genet Resour Crop Evol 57:667–677

    Article  CAS  Google Scholar 

  • Fu YB, Peterson G, Diederichsen A, Richards KW (2002) RAPD analysis of genetic relationships of seven flax species in the genus Linum L. Genet Resour Crop Evol 49:253–259

    Article  Google Scholar 

  • Gill KS, Yermanos DM (1967) Cytogenetic studies on the genus Linum I. Hybrids among taxa with 15 as the haploid chromosome number. Crop Sci 7:623–627

    Article  Google Scholar 

  • Grigoryeva VV (1988) The pollen grain morphology in the genus Linum (Linaceae) of the flora of the USSR. Russ Bot J 73(10):1409–1417

    Google Scholar 

  • Guerra M (2000) Patterns of heterochromatin distribution in plant chromosomes. Genet Mol Biol 23(4):1029–1041

    Article  Google Scholar 

  • Harris BD (1968) Chromosome numbers and evolution in North American species of Linum. Am J Bot 55(10):1197–1204

    Article  Google Scholar 

  • Howell WM, Black DA (1980) Controlled silver staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36:1014–1015

    Article  PubMed  CAS  Google Scholar 

  • Koo DH, Hur Y, Jin DC, Bang JW (2002) Karyotype analysis of a Korean cucumber cultivar (Cucumis sativus L. cv. Winter Long) using C-banding and bicolor fluorescence in situ hybridization. Mol Cells 13(3):413–418

    PubMed  CAS  Google Scholar 

  • Kutuzova SN, Gavrilyuk IP, Uggi EE (1999) Prospects of using protein markers to refine taxonomy and evolution of the genus Linum. Tr Bot Genet Sel 156:29–39

    Google Scholar 

  • Lemesh VA, Shut MV, Khotyleva LV (2005) RAPD analysis of interspecific polymorphism in flax (Genus Linum L.). Inf Vestn VOGiS 9(4):490–494

    Google Scholar 

  • McDill J, Repplinger M, Simpson BB, Kadereit JW (2009) The phylogeny of Linum and Linaceae subfamily Linoideae, with implications for their systematics, biogeography, and evolution of heterostyly. Syst Bot 34(2):386–405

    Article  Google Scholar 

  • Moroz OM, Tsymbalyuk ZM (2005) Palinomorphologycal characterization of sections Adenolinum, Dasylinum, Linopsis of genus Linum L. in the flora of Ukraine. Ukr Bot J 62(5):666–677

  • Mosquin T (1971) Biosystematic studies in the North American species of Linum section Adenolinum (Linaceae). Can J Bot 49:1379–1388

    Article  Google Scholar 

  • Muravenko OV, Amosova AV, Samatadze TE, Popov KV, Poletaev AI, Zelenin AV (2003) 9-Aminoacridine: an efficient reagent to improve human and plant chromosome banding patterns and to standardize chromosome image analysis. Cytometry 51(1):52–57

    Article  PubMed  Google Scholar 

  • Muravenko OV, Yurkevich OYu, Bolsheva NL, Samatadze TE, Nosova IV, Zelenina DA, Volkov AA, Popov KV, Zelenin AV (2009) Comparison of genomes of eight species of sections Linum and Adenolinum from the genus Linum based on chromosome banding, molecular markers and RAPD analysis. Genetica 135(3):245–255

    Article  PubMed  CAS  Google Scholar 

  • Nakamura R, Kitamura S, Inoue M, Ohmido N, Fukui K (2001) Karyotype analysis of Nicotiana kawakamii Y. Ohashi using DAPI banding and rDNA FISH. Theor Appl Genet 102:810–814

    Article  CAS  Google Scholar 

  • Navashin M (1934) Chromosomal alterations caused by hybridization and their bearing upon certain general genetic problems. Cytologia 5:169–203

    Article  Google Scholar 

  • Ockendon DJ (1968) Biosystematic studies in the Linum perenne group. New Phytol 67(4):787–813

    Article  Google Scholar 

  • Ockendon DJ (1971) Cytology and pollen morphology of natural and artificial tetraploids in the Linum perenne group. New Phytol 70(3):599–605

    Article  Google Scholar 

  • Ockendon DJ, Walters SM (1968) Linaceae. In: Tutin TG et al. (ed) Flora Europaea. Rosacea to Umbelliferaceae, vol 2. Cambrige, pp 206–211

  • Optasyuk OM (2006) Characterization of ultrastructure of the leaf surface in species of the genus Linum L. in the Ukrainian Flora. Ukr Bot J 63(6):805–815

    Google Scholar 

  • Optasyuk OM (2007) Genus Linum L. in the Flora of Ukraine. PhD Thesis, G. Kholodnyi Institute of Botany NAS of Ukraine, Kiev

  • Özcan T, Zorlu E (2009) A contribution to taxonomy of Turkish Linum based on seed surface patterns. Biologia 64(4):723–730

    Article  Google Scholar 

  • Pendleton RL, Kitchen SG, Mudge J, McArthur ED (2008) Origin of the flax cultivar ‘Appar’ and its position within the Linum perenne complex. Int J Plant Sci 169(3):445–453

    Article  Google Scholar 

  • Peterson DG, Lapitan NLV, Stack SM (1999) Localization of single- and low copy sequences on tomato synaptonemal complex spreads using fluorescence in situ hybridization (FISH). Genetics 152:427–439

    PubMed  CAS  Google Scholar 

  • Pignone D, Galasso I, Rossino R, Mezzanotte R (1995) Characterization of Dasypyrum villosum (L.) Candargy chromosomal chromatin by means of in situ restriction endonucleases, fluorochromes, silver staining and C-banding. Chrom Res 3:109–114

    Article  PubMed  CAS  Google Scholar 

  • Planchon JE (1848) Sur le famille des Lineés. London J Bot 7: 165–186, 473–501, 507–528

    Google Scholar 

  • Popov KV, Muravenko OV, Samatadze TE, Amosova AV, Zelenin AV (2001) Peculiarities of studying the heterochromatic regions of small plant chromosomes. Dokl Akad Nauk 381(4):562–565

    CAS  Google Scholar 

  • Raina SN, Mukai Y (1999) Detection of a variable number of 18S-5.8S-26S and 5S ribosomal DNA loci by fluorescent in situ hybridization in diploid and tetraploid Arachis species. Genome 42:52–59

    CAS  Google Scholar 

  • Raskina O, Belyayev A, Nevo E (2004) Quantum speciation in Aegilops: molecular cytogenetic evidence from rDNA clusters variability in natural populations. Proc Natl Acad Sci USA 101:14818–14823

    Article  PubMed  CAS  Google Scholar 

  • Raskina O, Barber J, Nevo E, Belyayev A (2008) Repetitive DNA and chromosomal rearrangements: speciation-related events in plant genome. Cytogen Gen Res 120:351–357

    Article  CAS  Google Scholar 

  • Ray C (1944) Cytological studies on the flax genus (Linum). Am J Bot 31:241–248

    Article  Google Scholar 

  • Rogers CM (1984) Linaceae. North American flora ser. II, part 12. The New York Botanical Garden, Bronx

  • Rogers SO, Bendich AJ (1985) Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol 5:69–76

    Article  CAS  Google Scholar 

  • Saad SI (1961a) Pollen morphology and sporoderm stratification in Linum. Grana 3(1):109–129

    Google Scholar 

  • Saad SI (1961b) Phylogenetic development in the apertural mechanisms of Linum pollen grains. Pollen Spores 3(1):33–45

    Google Scholar 

  • Schmidt TJ, Hemmati S, Klaes M, Konuklugil B, Mohagheghzadeh A, Ionkova I, Fuss E, Alfermann AW (2010) Lignans in flowering aerial parts of Linum species—chemodiversity in the light of systematics and phylogeny. Phytochemistry 71(14–15):1714–1728

    Article  PubMed  CAS  Google Scholar 

  • Schubert I, Wobus U (1985) In situ hybridization confirms jumping nucleolus organizing regions in Allium. Chromosoma 92:143–148

    Article  Google Scholar 

  • Schweizer D (1980) Fluorescent chromosome banding in plants; applications, mechanisms, and implications for chromosome structure. In: Davids DR, Hopwood DA (eds) The plant genome. The John Innes Institute, Norwich, UK, pp 61–71

  • Seetharam A (1972) Interspecific hybridization in Linum. Euphytica 21:489–495

    Article  Google Scholar 

  • Sizov IA (1970) On the evolution and genetics of Linum usitatissimum. Trudy Prikl Bot Genet Sel 42(1):3–19

    Google Scholar 

  • Svetlova AA (2005a) Systematics of the genus Linum (Linaceae) species of the section Adenolinum in the flora of Eastern Europe. Russ Bot J 90(7):1076–1087

    Google Scholar 

  • Svetlova AA (2005b) The taxonomic review of the section Adenolinum species of the genus Linum (Linaceae) of the flora of Northern Eurasia. Nov Sist Vyssh Rast 37:112–133

    Google Scholar 

  • Svetlova AA (2007) Genus Linum L. (Linaceae DC. ex Perleb.) in the Flora of Northern Eurasia: systematics, geography, evolution. PhD Thesis, V.L. Komarov Institute of Botany RAS, St. Petersburg

  • Svetlova AA (2008) Ultrasculpture of seeds and anatomical structure of seed coat of species from different sections of the genus Linum (Linaceae). Russ Bot J 93(11):1760–1771

    Google Scholar 

  • Svetlova AA (2009) The taxonomic review of the genus Linum (Linaceae) of the flora of Russia and adjacent states. Nov Sist Vyssh Rast 41:99–165

    Google Scholar 

  • Svetlova AA, Yakovleva OV (2006) Comparative anatomy of seed coat of some species from the genus Linum (Linaceae). Russ Bot J 91(12):112–133

    Google Scholar 

  • Velasco L, Goffman FD (2000) Tocopherol, plastochromanol and fatty acid patterns in the genus Linum. Plant Syst Evol 221:77–88

    Article  CAS  Google Scholar 

  • Vromans J (2006) Molecular genetic studies in flax (Linum usitatissimum L.). PhD Thesis, Wageningen University, Wageningen

  • Yakovleva OV, Bykova OP (2000) Ultrasculpture characters of mucilage cells mucilage of some species of the genus Alcea L. Rastitel'nye Resursy 36(3):1–8

    Google Scholar 

  • Yurkevich OYu (2008) Comparative study of genomes of species from sect. Linum, Adenolinum and Stellerolinum from the genus Linum based on C/DAPI-banding and fluorescent in situ hybridization (FISH). PhD Dissertation, Moscow: Engelhardt Institute of Molecular Biology RAS

  • Yuzepchuk SV (1949) Genus Linum—Linaceae Dumort. In: Shishkin BK (ed) Flora SSSR (Flora of the Soviet Union), vol 14. Moscow, Leningrad, pp 84–146

    Google Scholar 

  • Zelenina DA, Khrustaleva AM, Volkov AA (2006) Comparative study of the population structure and population assignment of sockeye salmon Oncorhynchus nerka from West Kamchatka based on RAPD–PCR and microsatellite polymorphism. Russ J Genet 42(5):563–572

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The research was supported by grants from the Russian State Foundation for Basic Research (10-04-01212; 11-08-00716), the Russian Ministry of Education and Science (contracts 16.512.11.2224 and 16.552.11.7034) and the Program of Russian Academy of Sciences (Living Nature—Dynamics of Genepools Conservation).

Author information

Authors and Affiliations

  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991, Moscow, Russia

    Olga Yu. Yurkevich, Nadezhda L. Bolsheva, Tatiana E. Samatadze, Olga A. Rachinskaya, Anna V. Kudryavtseva, Alexander V. Zelenin & Olga V. Muravenko

  2. Komarov Botanical Institute, Russian Academy of Sciences, 197376, St. Petersburg, Russia

    Alena A. Naumenko-Svetlova

  3. Russian Federal Institute for Fisheries and Oceanography, 107140, Moscow, Russia

    Daria A. Zelenina & Alexander A. Volkov

Authors
  1. Olga Yu. Yurkevich
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alena A. Naumenko-Svetlova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Nadezhda L. Bolsheva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tatiana E. Samatadze
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Olga A. Rachinskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Anna V. Kudryavtseva
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Daria A. Zelenina
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Alexander A. Volkov
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Alexander V. Zelenin
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Olga V. Muravenko
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Olga V. Muravenko.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yurkevich, O.Y., Naumenko-Svetlova, A.A., Bolsheva, N.L. et al. Investigation of genome polymorphism and seed coat anatomy of species of section Adenolinum from the genus Linum . Genet Resour Crop Evol 60, 661–676 (2013). https://doi.org/10.1007/s10722-012-9865-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10722-012-9865-z

Keywords

Search

Navigation