Research Article

Genetic Resources and Crop Evolution

, Volume 59, Issue 2, pp 253-260

First online:

EST-SSR cross-amplification and genetic similarity in Onobrychis genus

  • Samir DemdoumAffiliated withCentro de Investigación y Tecnología Agroalimentaria de Aragón Email author 
  • , Fernando MuñozAffiliated withCentro de Investigación y Tecnología Agroalimentaria de Aragón
  • , Ignacio DelgadoAffiliated withCentro de Investigación y Tecnología Agroalimentaria de Aragón
  • , José ValderrábanoAffiliated withCentro de Investigación y Tecnología Agroalimentaria de Aragón
  • , Ana WünschAffiliated withCentro de Investigación y Tecnología Agroalimentaria de Aragón

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Abstract

EST-SSR from Medicago truncatula Gaertn. and Glycine max (L.) Merr. were tested for transferability in various species of Onobrychis (O. pyrenaica Sennen, O. argentea Boiss. and O. viciifolia Scop.). Repeatable amplification was obtained for 81% of the microsatellites and 52% were polymorphic. Six selected SSRs from M. truncatula were used to fingerprint and estimate the genetic similarity of a set of 23 accessions of O. viciifolia. PCA analysis discriminated among the different Onobrychis species and the sainfoin accessions were clustered in a single major group. This grouping is discussed in terms of the history of cultivation of sainfoin in Spain. The selected SSRs will allow fingerprinting and genetic studies in Onobrychis species, solving the lack of available SSR markers in this species.

Keywords

Cultivar identification Fingerprinting Microsatellites Molecular markers Onobrychis Sainfoin SSR transferability