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Digestive enzyme activities of turbot (Scophthalmus maximus L.) during early developmental stages under culture condition

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Abstract

Digestive enzyme activities were analysed in turbot (Scophthalmus maximus) from hatching until 60 days after hatching (DAH). Trypsin sharply increased to the climax at 17 DAH and decreased until 31 DAH followed by a stable level thereafter. Amylase was determined at 4 DAH, reached the maximum value at 19 DAH and declined sharply to 39 DAH and remained at a low level thereafter, suggesting the carbohydrate component should remain at a low level in formulated diets. Pepsin was detected at 9 DAH and increased to 34 DAH and then remained at a stable level. The above results revealed pancreatic enzymes are no longer main enzymes for food digestion after the formation of functional stomach. Leucine–alanine peptidase (Leu-ala) and alkaline phosphatase (AP) and leucine aminopeptidase N (LAP) were found in newly hatched larvae. Both AP and LAP activities markedly increased to 23 DAH, decreased abruptly to 50 DAH and increased gradually to 60 DAH. Leu-ala reached the plateau from 23 to 39 DAH, followed by a decline to 46 DAH and an increase until 60 DAH. The brush border membrane (BBM)-bound enzyme activities increased from 30% at 31 DAH to 81% at 38 DAH of the total activities, indicating the maturation of intestinal tract.

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Acknowledgments

This study was funded by the earmarked fund for Modern Agro-industry Technology Research System (nycytx-50)and the 863 “the 11th Five-Year” Project of China (2006AA10A404) and Research Foundation for Advanced Talents (61071062006). We wish to express our sincere thanks to the staff of Oriental Ocean Sci-Tech Co., Ltd, for supplying experimental fish.

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Authors and Affiliations

  1. Yancheng Teachers University, Yancheng, 224002, People’s Republic of China

    X. H. Tong

  2. Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, People’s Republic of China

    S. H. Xu, Q. H. Liu, J. Li, Z. Z. Xiao & D. Y. Ma

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  1. X. H. Tong
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  2. S. H. Xu
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Correspondence to X. H. Tong.

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Tong, X.H., Xu, S.H., Liu, Q.H. et al. Digestive enzyme activities of turbot (Scophthalmus maximus L.) during early developmental stages under culture condition. Fish Physiol Biochem 38, 715–724 (2012). https://doi.org/10.1007/s10695-011-9553-5

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