Performance and grain yield stability of maize populations developed using marker-assisted recurrent selection and pedigree selection procedures
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- Beyene, Y., Semagn, K., Mugo, S. et al. Euphytica (2016) 208: 285. doi:10.1007/s10681-015-1590-1
A marker-assisted recurrent selection (MARS) program was undertaken in sub-Saharan Africa to improve grain yield under drought-stress in 10 biparental tropical maize populations. The objectives of the present study were to evaluate the performance of C1S2-derived hybrids obtained after three MARS cycles (one cycle of recombination (C1), followed by two generations of selfing (S2), and to study yield stability under both drought-stress (DS) and well-watered (WW) conditions. For each of the 10 populations, we evaluated hybrids developed by crossing 47–74 C1S2 lines advanced through MARS, the best five S5 lines developed through pedigree selection, and the founder parents with a single-cross tester from a complementary heterotic group. The hybrids and five commercial checks were evaluated in Kenya under 1–3 DS and 3–5 WW conditions with two replications. Combined across DS locations, the top 10 C1S2-derived hybrids from each of the 10 biparental populations produced 0.5–46.3 and 11.1–55.1 % higher mean grain yields than hybrids developed using pedigree selection and the commercial checks, respectively. Across WW locations, the best 10 hybrids derived from C1S2 of each population produced 3.4–13.3 and 7.9–36.5 % higher grain yields than hybrids derived using conventional pedigree breeding and the commercial checks, respectively. Mean days to anthesis of the best 10 C1S2 hybrids were comparable to those of hybrids developed using the pedigree method, the founder parents and the commercial checks, with a maximum difference of 3.5 days among the different groups. However, plant height was significantly (P < 0.01) different in most pairwise comparisons. Our results showed the superiority of MARS over pedigree selection for improving diverse tropical maize populations as sources of improved lines for stress-prone environments and thus MARS can be effectively integrated into mainstream maize breeding programs.
KeywordsAfrica Drought Molecular breeding SNP Rapid cycle recombination Testcross evaluation
Maize, a staple food in many sub-Saharan African countries, is grown by millions of resource-poor smallholder farmers. Between 2009 and 2011, maize was grown on more than 25 million hectares in sub-Saharan Africa (SSA) (Shiferaw et al. 2011), accounting for 7.5 % of global production. Average maize yield in SSA is 1.8 Mg ha−1, which is significantly lower than the yield in other maize-growing regions in the developing world. Recurrent drought is one of the major abiotic stresses in SSA, with approximately 22 % of mid-altitude/subtropical and 25 % of lowland tropical maize growing regions affected annually (Heisey and Edmeades 1999). Drought is expected to increase in severity due to the changing climate. Therefore, development and deployment of tropical maize germplasm with relevant agronomic and adaptive traits is key to enhance the food security and livelihoods of maize farming communities.
The ability to quickly develop germplasm with resistance to important abiotic and biotic stresses will be critical to the resilience of cropping systems in the face of climate change. Conventional breeding methods have a proven track record of improving tolerance for abiotic stresses (DTMA 2015). However, progress in breeding for drought tolerance using conventional approaches can be slow due to the polygenic nature of most stress-related traits, requiring accumulation of several quantitative trait loci (QTL) into adapted genetic backgrounds. In order to uncover and characterize genomic regions or QTLs associated with drought stress, several researchers (Veldboom and Lee 1996; Ribaut et al. 1997; Tuberosa et al. 2002; Almeida et al. 2013; Semagn et al. 2013) have reported a number of QTL for grain yield and other traits under both drought stress and well-watered conditions. In most cases, however, individual QTLs for highly polygenic traits explain only a small proportion of the phenotypic variance, and are highly dependent on genetic background and environmental conditions (Semagn et al. 2013).
Molecular marker-assisted breeding, including marker-assisted backcrossing (MABC), marker-assisted recurrent selection (MARS) and genomic selection (GS), in combination with high-throughput and precise phenotyping, doubled haploidy and year-round nurseries, can significantly accelerate the development of climate resilient maize germplasm (Prasanna et al. 2013; Xu et al. 2012). MARS is a breeding approach that aims to accumulate favorable alleles for a relatively large number of QTL in a given population using a subset of markers that are significantly associated with target traits (Bernardo 2008). GS incorporates all available marker information into a model to simultaneously predict genetic values of breeding progenies for selection, enabling accumulation of favorable alleles for major and minor QTL through multiple generations of recombination (Meuwissen et al. 2001). Recently, Beyene et al. (2015a) and Semagn et al. (2015) reported genetic gains achieved through GS and MARS in 8 and 10 tropical biparental maize populations, respectively. These studies involved genotyping 148–300 F2:3 (C0) progenies with 190–286 markers, evaluating testcrosses under WW and DS conditions and advancing selected lines using GS and MARS. In both studies, each population was represented by seed bulks containing equal amounts of seed of C0, C1, C2, C3, parents, F1s, and lines developed via pedigree selection. Five commercial checks were included for comparison. Beyene et al. (2015a) compared GS with pedigree selection across eight biparental tropical maize populations, and reported that the average gain per cycle from GS across eight populations was 0.086 t ha−1. Average grain yield of C3-derived hybrids was significantly higher than that of hybrids derived from C0. Hybrids derived from C3 produced 7.3 % higher grain yield than those developed through conventional pedigree breeding.
Semagn et al. (2015) reported that the average gain per cycle using MARS across 10 populations was 0.1837 t ha−1 under WW and 0.045 t ha−1 under DS conditions. Combined across DS environments, C3-derived hybrids produced 6.0, 8.3 and 37.8 % higher grain yields than hybrids derived from conventional pedigree breeding, parental lines and commercial checks, respectively. Across WW trials, the average grain yield of C3 hybrids was significantly (P < 0.05) higher than those hybrids derived C0, the pedigree method and the commercial checks. In both studies, the authors used a composite bulk to represent lines extracted from each selection cycle instead of using individual lines. In this study, we report the testcross performance of hybrids developed from 47 to 74 individual C1S2 lines instead of bulks derived from 10 MARS populations and evaluated under both managed drought-stress and optimum conditions. The objectives of the present study were: (1) to compare the overall gain in grain yield of all C1S2-derived hybrids with that of hybrids developed through conventional pedigree breeding method and (2) to compare yield stability of hybrids from a subset of selected populations developed through MARS and pedigree selection.
Materials and methods
Three cycles of MARS were completed on 10 tropical biparental populations. Detailed descriptions of the methodology and the results are given elsewhere (Beyene et al. 2015b) and briefly summarized here. Testcrosses were generated by crossing the F2:3 families (C0) from each population with a single-cross tester from a complementary heterotic group and evaluated under 2–3 managed drought stresses and 3–4 well-watered conditions in Kenya, Zimbabwe and Zambia. Each C0 population was genotyped with 190–225 SNPs and QTL analysis was performed for each population. Three selection cycles were conducted using a subset of 55–87 SNPs that were significantly associated with grain yield and anthesis-silking interval. Selected C0 families were first intermated to form Cycle 1 (C1), followed by selfing of superior C1 plants for two generations to form C1S1 and C1S2. At each recurrent selection cycle, selected individuals were genotyped with the significantly associated markers to increase favorable allele frequency. In each population, the top eight families from C0 were also advanced using a pedigree selection scheme. The various steps followed during MARS and pedigree phenotypic selection were illustrated by Beyene et al. (2015b).
Formation of testcrosses and phenotypic evaluation
From each population, 47–74 C1S2 lines developed through MARS, five S5 lines developed via phenotypic pedigree selection, and the two founder parents (P1 and P2) were crossed to a single-cross tester (CML395/CML444) at the Maize Research Station of Kenya Agriculture and Livestock Research Organization (KALRO), Kiboko, Kenya. This tester has proven to be useful in hybrid formation for subtropical and mid-altitude environments, and is also used as a parent in many commercial three-way-cross hybrids in SSA (Beyene et al. 2011, 2013). Experimental lines were used as female parents, and the single-cross tester was used as the male parent. Seeds were harvested and bulked within each female row plot for use in the testcross evaluation. Testcrosses of each population, together with five commercial checks (CZH0616, H513, WH505, DK8053, and Pioneer 3253), were evaluated in 3–5 WW and 1–3 DS locations in Kenya in 2013 and 2014. An alpha-lattice design with two replications per location was utilized for the trials. The DS trials were conducted during the dry (rain-free) season by withdrawing irrigation starting 2 weeks before flowering through harvest, whereas the WW trials were conducted during the rainy season, with supplemental irrigation applied as needed. Entries were planted in two-row plots, 5 m long, with 0.75 m spacing between rows and 0.25 m between hills. Two seeds per hill were initially planted and then thinned to one plant per hill at 3 weeks after emergence to obtain a final plant population density of 53,333 plants per hectare. Fertilizers were applied at the rate of 60 kg N and 60 kg P2O5 per ha as recommended for the area. Nitrogen was applied twice: at planting and 6 weeks after emergence. Fields were kept free of weeds by hand weeding.
Data on grain yield (GY), plant height (PH) and anthesis date (AD) were collected. AD was recorded as the number of days from planting to when 50 % of the plants had shed pollen. PH was measured as the distance from the base of the plant to the height of the first tassel branch. In DS trials, ears were harvested from each plot and all were shelled and weighed to determine the grain yield and percent grain moisture. In the WW experiments, ears harvested from each plot were weighed, sub-samples were shelled and grain moisture was determined on the sub-samples of grain. Grain yield was estimated assuming a shelling percentage of 80 % and adjusted to 125 g/kg moisture content.
Analysis of variance for grain yield, anthesis date and plant height within and across DS and WW locations was performed using the PROC MIXED procedure of SAS (SAS Institute 2009) considering locations and incomplete blocks as random effects and entries as fixed effects. For each population, the analyses were performed on all entries and also on groups of entries corresponding to test-crosses involving: (i) C1S2 lines; (ii) S5 lines extracted through phenotypic pedigree selection; (iii) commercial checks; and (iv) founder parents used for making the original populations. Contrasts were made to compare the performance of all C1S2 and the best 10 C1S2 hybrids versus five hybrids from the conventional pedigree scheme, founder parents and five commercial check hybrids. Stability analysis was done using the linear-bilinear site regression models (SREG) (Crossa and Cornelius 1997).
Summary of 10 biparental C1S2 populations evaluated in drought-stress (DS) and well-watered (WW) environments in Kenya
No. of C1S2
Number of WW locations
Number of DS locations
GY under WW (t ha−1)
GY under DS (t ha−1)
AD under WW (days)
AD under DS (days)
PH under WW (cm)
PH under DS (cm)
Heritability for GY under WW (DS)
Heritability for AD under WW (DS)
Heritability for PH under WW (DS)
Grain yield under drought stress conditions
Grain yield under well-watered conditions
As shown in Table 1 and Fig. 1, mean grain yield of testcrosses evaluated in WW environments varied from 5.77 t ha−1 (population 1020) to 7.91 t ha−1 (population 1017), with an overall average of 6.60 t ha−1. Mean grain yields of hybrids developed from all C1S2 lines within each population showed a 0.4–2.8 % advantage over those hybrids involving pedigree-derived lines in six populations (1008, 1017, 1018, 1021, 1023 and 1028), but showed a 1.3–6.6 % reduction compared to those of the other four populations (Fig. 1, Supplementary material S2). All contrasts between the mean grain yield of the best 10 hybrids formed from C1S2-derived lines versus the mean grain yield of the five hybrids involving pedigree derived lines, the commercial checks and the founder parents were significant (P ≤ 0.01) (Supplementary material S2). The best 10 hybrids of C1S2-derived lines per population produced (a) 3.4–13.3 % higher grain yield than those developed using lines through the pedigree method, (b) 9.9–36.5 % higher grain yield than the commercial checks (except population 1019, which showed a 1.1 % reduction), and (c) 8.1–27.0 % higher grain yield than the founder parents. Taking into account the time invested in developing the lines and using the grain yield of the founder parents as a baseline, the top 10 C1S2-derived hybrids on average produced 214.8 kg ha−1 year−1 under WW conditions, which is approximately double the 103.9 kg ha−1 year−1 grain yield observed for hybrids developed using the pedigree method.
Mean flowering date of the best 10 hybrids of C1S2-derived lines was generally similar to those hybrids formed from pedigree-derived lines, the founder parents and the commercial checks, with a maximum difference of 1–3 days (Fig. 2, Supplementary material S2). Pairwise comparisons of mean plant height of the different groups were significant for most populations (Supplementary material S2). The best 10 hybrids of C1S2-derived lines were 6–14.5 and 11–25.3 cm taller than the hybrids formed from pedigree derived lines and the commercial checks, respectively, in six populations (Fig. 3, Supplementary material S2). Compared with the commercial checks, the best 10 hybrids involving C1S2 derived lines were 11.0–19.2 cm shorter in three populations (1019, 1020, and 1021).
Grain yield stability
Entry code of the best 10 C1S2 derived hybrids and their grain yield (GY) (t ha−1) in water-stress and well-watered locations for each of the ten biparental populations
Conventional pedigree breeding has been used successfully to develop improved maize germplasm with abiotic and biotic stress resilience. Since 2007, CIMMYT and partners have used conventional breeding methods to develop and release over 200 drought tolerant hybrids and open-pollinated maize varieties in SSA under the framework of the Drought Tolerant Maize for Africa project (DTMA 2015). However, developing improved varieties using conventional breeding methods takes long, since many economically important traits require simultaneous accumulation of favorable alleles from several genomic regions. The use of molecular markers within breeding pipelines is widely, and successfully, employed by large private sector companies (Johnson 2004; Eathington et al. 2007). Use of molecular markers for tropical maize improvement in the developing world is however, constrained by several bottlenecks (Xu et al. 2012; Mba et al. 2012).
To facilitate the development and use of improved tropical maize germplasm, CIMMYT in collaboration with public and private partners conducted the largest public MARS and GS projects as part of both the Water Efficient Maize for Africa (WEMA) and DTMA projects. Based on the genetic gain data collected by these projects across multiple populations, each represented by a composite bulk of lines, we recently demonstrated the superiority of MARS for increasing grain yield under DS and WW conditions across diverse tropical maize populations without significantly affecting plant height and maturity of most populations (Beyene et al. 2015a; Semagn et al. 2015). When genetic gains for individual populations were considered, results indicated that different populations showed deferent responses to MARS, with the majority of the populations producing higher grain yields than those developed using conventional breeding methods. A smaller number of populations showed either similar or no gain in grain yield under both WW and DS conditions as compared with those developed through pedigree selection. A possible factor contributing to the lack of gain in grain yield from those populations may be the representation of every population by a composite bulk prior to testcross formation, which was implemented primarily to minimize the number of entries to be evaluated.
In the present study, hybrids developed by crossing 47–74 C1S2 lines from each of the 10 MARS populations with a single cross tester were evaluated. Combined across all DS locations and populations, the best 10 C1S2-derived hybrids produced 562, 750 and 916 kg ha−1 higher grain yield than pedigree-derived hybrids, commercial checks and founder parents, respectively. In WW locations, the best 10 C1S2-derived hybrids produced 583, 1305 and 1557 kg ha−1 more grain yield than pedigree-derived hybrids, commercial checks and founder parents, respectively.
As previously described by Beyene et al. (2015b) 3.5 years were required to develop S5 lines through pedigree selection and 4 years to develop C1S2 lines through MARS. Considering the number of years spent in developing the lines used in the study and taking the grain yield of the founder parents as the baseline data, the top 10 hybrids of C1S2-derived lines on average yielded 229 and 389.3 kg ha−1 year−1 in DS and WW conditions, respectively, which is higher than the yield of the hybrids formed from pedigree-derived lines (27.2 kg ha−1 year−1 under DS and 103.9 kg ha−1 year−1 under WW). The overall gains from the best 10 hybrids of C1S2-derived lines in the present study were higher than previous results reported by Beyene et al. (2015b) using composite bulk sampling. Although composite bulking of C1S2 lines prior to hybrid formation and their evaluation under multiple environments provided an overall idea of the genetic gain obtained through MARS over pedigree selection, results of the present study clearly demonstrate the value of evaluating every C1S2-derived hybrid to identify the best lines for further inbreeding and hybrid development. As shown in Supplementary material S2, the top 10 hybrids of C1S2-derived lines from each of the 10 biparental populations produced higher mean grain yields than hybrids developed using pedigree derived lines This result is similar to the findings of Eathington et al. (2007), who compared MARS and conventional selection in 248 North American and European maize breeding populations, and reported higher performance and yield index gains for MARS that was more than doubled compared to phenotypic selection. They also found that the MARS-derived lines were higher performing compared to conventionally selected lines.
A recent review of genetic gain studies from conventional pedigree selection conducted both in temperate and tropical maize germplasm reported highly variable results (Edmeades 2013). In SSA, preliminary estimates of yield gains from conventional selection revealed 39–80 kg ha−1 year−1 under optimal conditions, but only 18 kg ha−1 year−1 under drought stress (Edmeades 2013). A recent study using 67 hybrids developed at CIMMYT and released between 2000 and 2011 showed genetic gains of 32 and 109 kg ha−1 year−1 for grain yield under managed drought and well-watered conditions, respectively (Masuka et al. 2015, submitted). Therefore, the overall average gain obtained under DS (229 kg ha−1 year−1) and WW (389.3 kg ha−1 year−1) in the current study was three to seven times higher than that reported from conventional phenotypic selection in SSA. Genetic gains obtained through pedigree breeding in the current study (27.2 and 103.9 kg ha−1 year−1 under DS and WW conditions, respectively) were similar to estimates reported in the literature in SSA (Edmeades 2013).
Edmeades et al. (2004) reported that the phenotypic correlation between elite hybrid yields under stress versus under well-watered conditions declined as stress intensified, reaching 0.35 (r2 = 0.12) when yield reductions reached 50 %. They suggested that stress adaptive mechanisms were not exposed until yields had been reduced by 30–50 % under stress. In the current study, the average grain yield were 6.60 t ha−1 under WW condition and 2.61 t ha−1 under DS condition, which represented a 61 % yield reduction, approaching the 70 % yield reduction typically targeted by CIMMYT in managed drought stress experiments in SSA (Bänziger et al. 2000). Accordingly, the best 10 C1C2 lines identified in these studies from each population may have adaptive traits for drought tolerance, which could be utilized as sources of drought tolerance in maize breeding.
Relative differences in genetic gains observed between MARS and pedigree selection were much higher under DS conditions than under WW conditions), suggesting that MARS could accelerate the pace of improvement, for complex traits such as drought tolerance. These results agree with previous reports (Eathington et al. 2007; Xu and Crouch 2008; Beyene et al. 2015b), indicating that MARS can be more efficient and effective than phenotypic selection, and could improve genetic gains for complex traits like drought tolerance in tropical maize breeding programs.
Since drought incidence and severity vary considerably among years and within fields, it is important to develop hybrids that are able to withstand drought stress throughout the growing season, but also have no yield penalty under optimum conditions. Hybrids performing well under both DS and WW conditions were identified in this study (Table 2). For example, entries 14 and 25 in population 1008 and entries 27, 36, and 37 in population 1016 are among the top performing hybrids under both DS and WW conditions. Therefore, the parents of these hybrids need to be fixed through generation of inbreeding to develop hybrids that will perform better both under drought stress and well water conditions.
The results reported in this paper were generated as part of the Water Efficient Maize for Africa project, funded by the Bill and Melinda Gate and the Howard G Buffet Foundations. The authors would like to thank, Joel Mbithi, Patrick Gichobi, David Karuri and Gabriel Ambani for data collection at the various experimental sites.
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