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Gene-based molecular marker system for multiple disease resistances in tomato against Tomato yellow leaf curl virus, late blight, and verticillium wilt

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Abstract

Marker assisted selection (MAS) for disease resistance is widely applied in practical tomato breeding programs in the public and private sectors. Due to its commercial value and importance as a model crop, tomato has taken the lead in MAS among the horticultural crops. Tomato yellow leaf curl virus, which is transmitted by the whitefly (Bemisia tabaci), is a major threat to tomato production worldwide. The Ty1 and Ty3 resistance loci originated from Solanum chilense LA1969 and LA1932/LA2779, respectively. Recently, the gene responsible for Ty1 resistance was identified as a DFDGD-class RNA-dependent RNA polymerase and was demonstrated to be allelic with Ty3 resistance. The Ph3 resistance locus from S. pimpinellifolium (L3708), which confers incomplete resistance against a widerange of Phytophthora infestans isolates, is considered the most effective source of resistance against tomato late blight. A coiled-coil nucleotide-binding leucine-rich repeat gene on chromosome 9 was determined to be responsible for Ph3 resistance. Resistance against verticillium wilt diseases in tomato is conferred by the Ve locus that contains two closely linked, inversely oriented genes: Ve1 and Ve2. The Ve locus provides resistance against Verticillium alboatrum race 1 and encodes an extracellular leucine-rich repeat receptor-like protein class of disease resistance proteins. We developed reliable and comprehensive molecular markers based on either the single nucleotide polymorphisms or insertions/deletions directly responsible for the resistance provided by the Ty1, Ph3, and Ve1 loci. These gene-based functional molecular markers are expected to enhance the effectiveness and accuracy of MAS for disease resistance in tomato breeding programs.

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Acknowledgments

This work was supported by Golden Seed Project (Center for Horticultural Seed Development, 2013003-04-2-SBG10) funded by the Ministry of Agriculture, Food and Rural Affairs, and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2014R1A1A1A05006972).

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  1. Hyun Jung Kim & Inhwa Yeam

    Present address: Department of Eco-Friendly Horticulture, Cheonam Yonam College, Cheonansi, Chungcheongnamdo, Korea

Authors and Affiliations

  1. Department of Horticulture and Breeding, Andong National University, Andongsi, Gyeongsangbukdo, 760-749, Republic of Korea

    Jungsu Jung & Inhwa Yeam

  2. Institute of Agricultural Science and Technology, Andong National University, Andongsi, Gyeongsangbukdo, 760-749, Republic of Korea

    Hyun Jung Kim & Inhwa Yeam

  3. Department of Horticultural Science, Kyungpook National University, Daegu, Republic of Korea

    Je Min Lee

  4. Department of Plant Science, College of Agriculture and Life Sciences, Kyung Hee University, Seoul, Republic of Korea

    Chang Sik Oh & Hyung-Jin Lee

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  1. Jungsu Jung
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Correspondence to Inhwa Yeam.

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Jungsu Jung and Hyun Jung Kim are co-first authors.

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Jung, J., Kim, H.J., Lee, J.M. et al. Gene-based molecular marker system for multiple disease resistances in tomato against Tomato yellow leaf curl virus, late blight, and verticillium wilt. Euphytica 205, 599–613 (2015). https://doi.org/10.1007/s10681-015-1442-z

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