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Identification of defense-related genes in banana roots infected by Fusarium oxysporum f. sp. cubense tropical race 4

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Abstract

Fusarium oxysporum f. sp. cubense tropical race 4 is a lethal pathogen, affecting banana production by causing vascular wilt. To date, the molecular basis of resistant cultivars is largely unknown. In this study, suppression subtractive hybridization (SSH) libraries were constructed for the roots of two iso-line cultivars—a Foc-susceptible cultivar and its tolerant mutant—using both uninfected controls and plants artificially challenged with pathogen conidiospores. Unigenes from SSH libraries were used to develop a cDNA array, and then microarray analysis was carried out to verify those genes exhibiting differential expression. A total of 257 unigenes was obtained from the SSH libraries and microarray detection confirmed that most of them were differentially expressed. Of these, 116 unigenes had matched proteins in gene ontology and were classified into 38 different functional groups. The transcript levels of up to 19 putative defense genes were followed for a whole infection period in four cultivars differing in resistance level. At the early stages of infection (within 48 h), nineteen genes varied by a factor of more than three, subsequently only four genes (Pectinesterase inhibitor, PEI; Peroxidase, POD; Pathogenesis-related protein 1, PR1; and Pathogenesis-related protein 3, PR3) maintained their up-regulation. This indicates that anti-oxidation, cell wall modification and synthesis of anti-fungal proteins are the most important aspects to study if we are to understand the Foc resistance mechanism in commercial banana plants.

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Acknowledgments

This study was financially supported by the National Natural Science Foundation of China (No. 31101535, 31261140366), Hainan Provincial Natural Science Foundation of China (313056), the Earmarked Fund for Modern Agro-industry Technology Research System (nycytx-33-20) and the Chinese Special Fund of Basic Scientific Research Project for State Level and Public Welfare-scientific Research Institutes (1251022011002). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We’d really like to thank Dr. Joelle Hoggan for helping us polish the manuscript.

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The authors declare that they have no conflict of interests.

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Authors and Affiliations

  1. Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, 524091, China

    Weiming Li, Wei Wang, Yulin Hu, Yiwei Mo, Dequan Sun, Shengyou Shi & Jianghui Xie

  2. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, The Chinese Academy of Sciences, Guangzhou, 510650, China

    Xuejun Ge & Wei Wu

  3. College of Horticulture, South China Agricultural University, Guangzhou, 510642, China

    Weiming Li

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  1. Weiming Li
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  2. Xuejun Ge
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  3. Wei Wu
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  4. Wei Wang
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  5. Yulin Hu
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  7. Dequan Sun
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Correspondence to Jianghui Xie.

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Li, W., Ge, X., Wu, W. et al. Identification of defense-related genes in banana roots infected by Fusarium oxysporum f. sp. cubense tropical race 4. Euphytica 205, 837–849 (2015). https://doi.org/10.1007/s10681-015-1418-z

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