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Mapping QTL for cotton fiber quality traits using simple sequence repeat markers, conserved intron-scanning primers, and transcript-derived fragments

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Abstract

Cotton is an important fiber crop worldwide. Improved fiber quality is a driving force for cotton genetic studies and breeding. A BC1 population containing 115 individuals from a cross between Gossypium hirsutum cv. CCRI8 and G. barbadense cv. Pima 90-53 was established, and 519 simple sequence repeat (SSR) markers, two conserved intron-scanning primers (CISPs), and transcript-derived fragments (TDFs) amplified from 156 ApoI/TaqI selective primer combinations were used to construct a genetic linkage map. The map included 579 markers distributed on 56 linkage groups. Accounting for 83.4 % of the cotton genome, it covered 4,168.72 cM, with an average distance of 7.19 cM between markers. Lengths of the linkage groups ranged from 1.25 to 255.79 cM, with 2 to 44 markers per group. Of these 56 groups, 43 were assigned to 26 chromosomes, with the remaining 13 unknown. Based on this newly constructed map of tetraploid cotton, we performed quantitative trait loci (QTL) mapping and analysis of fiber quality traits from the BC1 and its derived BC1F2 lines. A total of 44 fiber quality QTL were detected on 17 chromosomes, explaining 7.72–23.73 % of the phenotypic variation. Pima 90-53 offered 13 QTL alleles with positive additive effects and four with negative additive effects for fiber quality traits. The results from this study may provide useful information for breeders to transfer desirable fiber traits from cotton types, such as the Sea Island strain, to Upland cotton that is primarily cultivated currently.

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Acknowledgments

This work was financially supported by the National High Technology Research and Development Program of China (863 Program; grant no. 2012AA101108-02) and the National Natural Science Foundation of China (Grant No. 31071467). The authors are grateful to Priscilla Licht for critical reading of this manuscript.

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Authors and Affiliations

  1. North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Key Laboratory for Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Lekai South Street 2596, Baoding, 071001, Hebei, China

    Xinlei Yang, Xiaodong Zhou, Xingfen Wang, Zhikun Li, Yan Zhang, Hengwei Liu, Liqiang Wu, Guiyin Zhang, Guijun Yan & Zhiying Ma

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  1. Xinlei Yang
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  2. Xiaodong Zhou
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  5. Yan Zhang
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  6. Hengwei Liu
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  10. Zhiying Ma
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Correspondence to Zhiying Ma.

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Yang, X., Zhou, X., Wang, X. et al. Mapping QTL for cotton fiber quality traits using simple sequence repeat markers, conserved intron-scanning primers, and transcript-derived fragments. Euphytica 201, 215–230 (2015). https://doi.org/10.1007/s10681-014-1194-1

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