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Identification of quantitative trait loci for downy mildew resistance in cucumber (Cucumis sativus L.)

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Abstract

Downy mildew, caused by Pseudoperonospora cubensis (Berk. & Curt.) Rostov, is one of the most economically important foliar diseases in cucumber (Cucumis sativus L.). Cucumber line CS-PMR1, derived from self-pollination of USDA Plant Introduction 197088, has a high level of resistance to downy mildew and is considered to be promising breeding material. In this study, we performed quantitative trait locus (QTL) analysis for downy mildew resistance using 111 recombinant inbred lines (RILs) derived from a cross between CS-PMR1 and the old Japanese cultivar Santou, which exhibits moderate resistance. The resistance of the RILs and their parents was evaluated by diverse methods using different plant organs (cotyledons, true leaves), stages (seedlings and adult plants), and evaluation criteria (lesion expansion and extent of sporulation). The high resistance of CS-PMR1 was associated with many QTLs with relatively small effects, whereas the moderate resistance of Santou was associated with one major QTL and possibly two others with relatively small effects. In all assays, the major QTL at which the Santou allele was associated with increased resistance had the largest effect. This QTL allele from Santou and several of the most effective QTL alleles identified in CS-PMR1 should be highest priority for selection to efficiently breed new cultivars that carry adequate levels of downy mildew resistance.

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Acknowledgments

We thank N. Nishimura, F. Hori, D. Yamashita, S. Negoro, T. Yamakawa, K. Takeuchi, M. Shindo, M. Wakabayashi, M. Sugao, Y. Taki, A. Suzuki, and T. Yamada for their technical assistance. This work was supported by NARO Research Project No. 211 ‘Establishment of Integrated Basis for Development and Application of Advanced Tools for DNA Marker-Assisted Selection in Horticultural Crops’.

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Author notes

  1. Yosuke Yoshioka

    Present address: Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan

  2. Yoshiteru Sakata

    Present address: NARO Agricultural Research Center for Kyushu Okinawa Region, Kurume, Fukuoka, 839-8503, Japan

Authors and Affiliations

  1. NARO Institute of Vegetable and Tea Science, 360 Kusawa, Ano, Tsu, Mie, 514-2392, Japan

    Yosuke Yoshioka, Yoshiteru Sakata, Mitsuhiro Sugiyama & Nobuko Fukino

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  1. Yosuke Yoshioka
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  2. Yoshiteru Sakata
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  3. Mitsuhiro Sugiyama
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  4. Nobuko Fukino
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Correspondence to Nobuko Fukino.

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Yoshioka, Y., Sakata, Y., Sugiyama, M. et al. Identification of quantitative trait loci for downy mildew resistance in cucumber (Cucumis sativus L.). Euphytica 198, 265–276 (2014). https://doi.org/10.1007/s10681-014-1102-8

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  • DOI: https://doi.org/10.1007/s10681-014-1102-8

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