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Morphological and molecular identification of potato and cereal cyst nematode isolates from Algeria and their phylogenetic relationships with other populations from distant theirgeographical areas

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An Erratum to this article was published on 23 November 2016

Abstract

A nematode survey conducted in 2013 in Algeria, revealed that potato cyst nematodes (PCN) and cereal cyst nematodes (CCN) are widely distributed in several potato and cereal growing regions of the country. Sixteen PCN populations from five localities and five CCN populations from four of these localities were collected and characterized at the morphological and molecular levels. The PCN populations were identified as Globodera rostochiensis and G. pallida occurring separately or in mixed populations. Two species of CCN were detected. Heterodera avenae was found in four localities, whereas H. hordecalis only in one locality in association with H. avenae. The morphological and morphometric identification of PCN and CCN was confirmed by diagnostic ITS-RFLP profiles and sequencing. Phylogenetic analysis of the ITS, D2-D3 expansion domains of the 28S rRNA gene and 18S rRNA gene was made for PCN and CCN populations. Globodera pallida and G. rostochiensis from Algeria show great similarity with European and South American populations. Because of the high divergence among Algerian populations of G. pallida and G. rostochiensis it can be assumed that they were multi-introduced in Algeria. The most divergent population of G. pallida, that formed a well-separated group with some populations from Chile and Peru, suggests a later or independent introduction of this population into Algeria. Heterodera avenae and H. hordecalis formed a well-supported cluster with the corresponding populations.

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Acknowledgments

The authors wish to thank Dr. Nicola Greco for critical review of the manuscript and helpful suggestions and discussions.

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Authors and Affiliations

  1. University of Khemis Miliana, Theniet El Had Road, Ain Defla, Algeria

    Nadia Tirchi & Aissa Mokabli

  2. Istituto per la Protezione Sostenibile delle Piante, Consiglio Nazionale delle Ricerche (CNR), Via Amendola 122/D, 70126, Bari, Italy

    Alberto Troccoli, Elena Fanelli & Francesca De Luca

  3. National Upper School of Agronomy, El Harrach, Algiers, Algeria

    Fazia Mouhouche

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  1. Nadia Tirchi
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  2. Alberto Troccoli
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Correspondence to Francesca De Luca.

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An erratum to this article is available at http://dx.doi.org/10.1007/s10658-016-1100-x.

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Tirchi, N., Troccoli, A., Fanelli, E. et al. Morphological and molecular identification of potato and cereal cyst nematode isolates from Algeria and their phylogenetic relationships with other populations from distant theirgeographical areas. Eur J Plant Pathol 146, 861–880 (2016). https://doi.org/10.1007/s10658-016-0965-z

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