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Duality of acibenzolar-S-methyl in the inhibition of pathogen growth and induction of resistance during the interaction tomato/vertcillium dahliae

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Abstract

The plant defense activator, acibenzolar-S-methyl (ASM) was tested for its ability to protect tomato seedlings against verticillium wilt caused by Verticillium dahliae. ASM exhibited in vitro an antifungal activity in dose-dependent manner against three strains of the pathogen with the less virulent strain SH being the most inhibited. This inhibition of mycelial growth reached 75 % when ASM was applied at concentrations higher than 50 μg ml−1. ASM also induced an elevated protection against the strain SH in the greenhouse when it was applied twice as foliar sprays at 100 μg ml−1 before root inoculation. It reduced leaf alteration index by 67 %, vessel browning index and growth alteration by 80 %. The plant defense activator markedly enhanced accumulation of H2O2. Furthermore, ASM primed tomato seedlings for enhanced activity of peroxidase and polyphenol oxidase. These results suggest that ASM protects tomato from V. dahlia directly by inhibiting the growth of pathogen and indirectly by activating plant defenses responses.

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References

  • Alexieva, V., Sergiev, I., Mapelli, S., & Karanov, E. (2001). The effect of drought and ultraviolet radiation growth and stress markers in pea and wheat. Plant, Cell & Environment, 24(12), 1337–1344.

    Article  CAS  Google Scholar 

  • Anterola, A. M., & Lewis, N. G. (2002). Trends in lignin modification: a comprehensive analysis of the effects of genetic manipulations/mutations on lignification and vascular integrity. Phytochemistry, 61(3), 221–294.

    Article  CAS  PubMed  Google Scholar 

  • Benhamou, N., & and Belanger, R. (1998). Benzothiadiazole-mediated induced resistance to Fusarium oxysporum f. sp. radicis-lycopersici in tomato. Plant Physiology 118 (4), 1203–1212.

  • Bradford, M. M. (1976). A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1–2), 248–254.

    Article  CAS  PubMed  Google Scholar 

  • Buonaurio, R., Scarponi, L., Ferrara, M., Sidoti, P., & Bertona, A. (2002). Induction of systemic acquired resistance in pepper plants by acibenzolar-S-methyl against bacterial spot disease. European Journal of Plant Pathology, 108(1), 41–49.

    Article  CAS  Google Scholar 

  • Cherrab, M., Bennani, A., Charest, P. M., & Serrhini, M. N. (2002). Pathogenecity and vegetative compatibility of verticillium dahlia kleb. Isolates from olives in morocco. Journal of Phytopathology, 150(11–12), 703–709.

    Article  Google Scholar 

  • Cole, D. L. (1999). The efficacy of acibenzolar-S-methyl, an inducer of systemic acquired resistance, against bacterial and fungal diseases of tobacco. Crop Protection, 18(4), 267–273.

    Article  CAS  Google Scholar 

  • Conrath, U., Beckers, G. J. M., Flors, V., García-Agustín, P., Jakab, G., Mauch, F., Newman, M. A., Pieterse, C. M. J., Poinssot, B., Pozo, M. J., Pugin, A., Schaffrath, U., Jurriaan, T., Wendehenne, D., Zimmerli, L., & Mauch-Mani, B. (2006). Priming: getting ready for battle. Molecular Plant-Microbe Interactions, 19(10), 1062–1071.

    Article  CAS  PubMed  Google Scholar 

  • Cools, H. J., & Ishii, H. (2002). Pre-treatment of cucumber plants with acibenzolar-S-methyl systemically primes a phenyl alanine ammonia lysae gene (PAL1) for enhanced expression upon attack with a pathogenic fungus. Physiological and Molecular Plant Pathology, 61, 273–280.

    Article  CAS  Google Scholar 

  • Daayf, F., Nicole, M., & Geiger, J. P. (1995). Differentiation of verticillium dahlia on the basis of vegetative compatibility and pathogenicity on cotton. European Journal of Plant Pathology, 101(1), 69–79.

    Article  Google Scholar 

  • Durrant, W. E., & Dong, X. (2004). Systemic acquired resistance. Annual Review of Phytopathology, 42, 185–209.

    Article  CAS  PubMed  Google Scholar 

  • Erwin, D. C., Tsai, S. D., & Khan, R. A. (1976). Reduction of the severity of verticillium wilt of cotton by the growth retardant, tributyl (5 chloro-2-thienyl methyl) phosphonium chloride. Phytopathology, 66, 106–110.

    Article  CAS  Google Scholar 

  • Faize, M., Faize, L., Koike, N., Ishizaka, M., & Ishii, H. (2004). Acibenzolar-S-methyl-induced resistance to Japanese pear scab is associated with potentiation of multiple defense responses. Phytopathology, 94(6), 604–612.

    Article  CAS  PubMed  Google Scholar 

  • Faize, M., Faize, L., & Ishii, H. (2009). Gene expression during acibenzolar-S-methyl-induced priming for potentiated responses to venturia nashicola in Japanese pear. Journal of Phytopathology, 157(3), 137–144.

    Article  CAS  Google Scholar 

  • Foyer, C. H., & Noctor, G. (2005). Redox homeostasis and antioxidant signaling: a metabolic interface between stress perception and physiological responses. Plant Cell, 17(7), 1866–1875.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Friedrich, L., Lawton, K., Ruess, W., Masner, P., Specker, N., Rella, M. G., Meier, B., Dincher, S., Staub. T., Uknes S., Metraux J. P., Kessmann, H., & Ryals, J. (1996). A benzothiadiazole derivative induces systemic acquired resistance in tobacco. The Plant Journal 10 (1), 61–70.

  • Ishii, H., Tomita, Y., Horio, T., Narusaka, Y., Nakazawa, Y., Nishimura, K., & Iwamoto, S. (1999). Induced resistance of acibenzolar-S-methyl (CGA 245704) to cucumber and Japanese pear diseases. European Journal of Plant Pathology, 105, 77–85.

    Article  CAS  Google Scholar 

  • Katz, V., Thulke, O. U., & Conrath, U. A. (1998). Benzothiadiazole primes parsley cells for augmented elicitation of defense responses. Plant Physiology, 117(4), 1333–1339.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Klosterman, S. J., Atallah, Z. K., Vallad, G. E., & Subbarao, K. V. (2009). Diversity, pathogenicity, and management of verticillium species. Annual Review of Phytopathology, 47, 39–62.

    Article  CAS  PubMed  Google Scholar 

  • Li, L., & Steffens, J. C. (2002). Overexpression of polyphenol oxidase in transgenic tomato plants results in enhanced bacterial disease resistance. Planta, 215(2), 239–247.

    Article  CAS  PubMed  Google Scholar 

  • Louws, F. J., Wilson, M., Campbell, H. L., Cuppels, D. A., Jones, J. B., Shoemaker, P. B., Sahin, F., & Miller, S. A. (2001). Field control of bacterial spot and bacterial speck of tomato using a plant activator. Plant Disease, 85(5), 481–488.

    Article  CAS  Google Scholar 

  • Mandal, B., Mandal, S., Csinos, A. S., Martinez, N., Culbreath, A. K., & Pappu, H. R. (2008). Biological and molecular analyses of the acibenzolar-S-methyl-induced systemic acquired resistance in flue-cured tobacco against tomato spotted wilt virus. Phytopathology, 98(2), 196–204.

    Article  CAS  PubMed  Google Scholar 

  • Masia, A., Ventura, M., Gemma, H., & Sansavini, S. (1998). Effect of some plant growth regulator treatments on apple fruit ripening. Plant Growth Regulation, 25(2), 127–134.

    Article  CAS  Google Scholar 

  • Matheron, M. E., & Porchas, M. (2002). Suppression of phytophthora root and crown rot on pepper plant treated with acibenzolar-S-methyl. Plant Disease, 86(3), 292–297.

    Article  CAS  Google Scholar 

  • Moerschbacher, B., Heck, B., Kogel, K. H., Obst, O., & Reisener, H. G. (1986). An elicitor of the hypersensitive lignification response in wheat leaves isolated from the rust fungus Puccinia graminis f. sp. tritici. II. Induction of enzymes correlated with the biosynthesis of lignin. Zeitschrift fur Naturforschung 41c: 839–844.

  • Muñoz, Z., & Moret, A. (2010). Sensitivity of botrytis cinerae to chitosan and cibenzolar-S-methyl. Pest Management Science, 66(9), 974–979.

    Article  PubMed  Google Scholar 

  • Nicholoson, R. L., & Hammerschmidt, R. (1992). Phenolic compounds and their role in resistance. Annual Review of Phytopathology, 30, 369–389.

    Article  Google Scholar 

  • Pappu, H. R., Csinos, A. S., McPherson, R. M., Jones, D. C., & Stephenson, M. G. (2000). Effect of acibenzolar-S-methyl and imidacloprid on suppression of tomato spotted wilt tospovirus in flue-cured tobacco. Crop Protection, 19(5), 349–354.

    Article  CAS  Google Scholar 

  • Rekanovic, E., Milijasevic, S., Todorovic, B., & Potocnik, I. (2007). Possibilities of biological and chemical control of verticillium wilt in pepper. Phytoparasitica, 35(5), 436–441.

    Article  CAS  Google Scholar 

  • Soylu, S., Baysal, O., & Soylu, E. M. (2003). Induction of disease resistance by the plant activator, acibenzolar-S-methyl (ASM), against bacterial canker (Clavibacter michiganensis subsp. Michiganensis) in tomato seedlings. Plant Science, 105(5), 1069–1075.

    Article  Google Scholar 

  • Talboys, P. W. (1984). Chemical control of verticillium wilts. Phytopathologia Mediterranea, 23, 163–175.

    Google Scholar 

  • Tian, L., Wang, K. R., & Lu, J. Y. (1998). Effect of carbendazim and tricyclazole on microsclerotia and melanin formation of Verticillium dahliae. Acta Physica Sinica, 28, 263–268.

    Google Scholar 

  • Tripathi, D., & Pappu, H. R. (2015). Evaluation of acibenzolar-S-methyl-induced resistance against iris yellow spot tospovirus. European Journal of Plant Pathology, 142(4), 855–864.

    Article  CAS  Google Scholar 

  • Wendehenne, D., Durner, J., Zhixiang, C., & Klessig, D. F. (1998). Benzothiadiazole, an inducer of plant defenses, inhibits catalase and ascorbate peroxidase. Phytochemistry, 47(4), 651–657.

    Article  CAS  Google Scholar 

  • Wilhel, M., & Taylor, J. B. (1965). Control of verticillium wilt of olive through natural recovery and resistance. Phytopathology, 55, 310–316.

    Google Scholar 

Download references

Acknowledgments

This work was supported by the Moroccan Ministry of Higher Education and Research. Many thanks to Dr. S. Qsaib for his technical assistance.

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Authors and Affiliations

  1. Laboratory of Plant Physiology, Biotechnology and Ecosystem Valorisation, Faculty of Sciences, University Chouaib Doukkali, El Jadida, Morocco

    Hanane Zine, Lalla Aicha Rifai, Mohamed Faize, Amal Smaili, Kacem Makroum, Malika Belfaiza, El Mustapha Kabil & Tayeb Koussa

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  1. Hanane Zine
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  2. Lalla Aicha Rifai
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  3. Mohamed Faize
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  4. Amal Smaili
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  5. Kacem Makroum
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  6. Malika Belfaiza
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  7. El Mustapha Kabil
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  8. Tayeb Koussa
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Correspondence to Mohamed Faize.

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Zine, H., Rifai, L.A., Faize, M. et al. Duality of acibenzolar-S-methyl in the inhibition of pathogen growth and induction of resistance during the interaction tomato/vertcillium dahliae . Eur J Plant Pathol 145, 61–69 (2016). https://doi.org/10.1007/s10658-015-0813-6

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  • DOI: https://doi.org/10.1007/s10658-015-0813-6

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