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Refining a method for ascospore viability testing in overwintering chasmothecia of Erysiphe necator

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Abstract

The vital stain fluorescein diacetate (FDA) has long been used to assess the viability of Erysiphe necator ascospores in chasmothecia. To determine the viability of animal, plant and other cells, more complex staining procedures, for example simultaneous double-staining procedures based on the use of FDA and propidium iodide (PI), have also been widely used. This paper reports a refined, detailed version of this method and its limitations, where PI was replaced with ethidium bromide (EtBr), to be used in testing the viability of E. necator ascospores during overwintering in chasmothecia.

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Acknowledgments

This research was partially supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of TÁMOP-4.2.2D-15/1/Konv-2015-003, and by a grant of the Hungarian Scientific Research Fund (OTKA NN 100415).

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Authors and Affiliations

  1. Institute of Biology, Department of Plant Anatomy, Eötvös Loránd University, Pázmány Péter sétány 1/C, Budapest, H-1117, Hungary

    Pál Vági

  2. Department of Sustainable Crop Production, Università Cattolica del Sacro Cuore, Via E. Parmense 84, 29122, Piacenza, Italy

    Tito Caffi

  3. Eszterházy Károly University of Applied Sciences, Regional Research Centre, Leányka utca 6, Eger, H-3300, Hungary

    Kálmán Zoltán Váczy

  4. Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences (MTA-ATK), P.O. Box 102, Budapest, H-1525, Hungary

    Pál Vági, Márk Z. Németh & Levente Kiss

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  1. Pál Vági
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  2. Tito Caffi
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  3. Kálmán Zoltán Váczy
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  4. Márk Z. Németh
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  5. Levente Kiss
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Correspondence to Levente Kiss.

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Vági, P., Caffi, T., Váczy, K.Z. et al. Refining a method for ascospore viability testing in overwintering chasmothecia of Erysiphe necator . Eur J Plant Pathol 144, 799–802 (2016). https://doi.org/10.1007/s10658-015-0797-2

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  • DOI: https://doi.org/10.1007/s10658-015-0797-2

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