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Activity-guided separation of Chromolaena odorata leaf extract reveals fractions with rice disease-reducing properties

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Abstract

An aqueous extract from the plant Chromolaena odorata [syn Eupatorium odoratum], Eupatorieae, Asteraceae, protected rice against sheath blight by induced resistance. As an initial step towards identification of the specific disease-reducing compounds, leaves from C. odorata were extracted with water and methanol and the extracts separated using a group separation system followed by analysis using capillary electrophoresis. The fractions from the extracts were tested in vitro and in planta using Bipolaris oryzae (cause of brown spot of rice) to test for their potential to reduce disease severity. Activity-guided separation of the C. odorata extracts indicated that compounds with activity could, at least partly, be isolated on a weakly acidic cation exchange column. Further purification yielded fractions with disease reducing effects of up to 72 % at 15 days after inoculation. Activity was found both in methanol and water extracts, indicating that the bioactive compound(s) are hydrophilic, low molecular weight compounds. The disease-reducing fractions did not display any direct antimicrobial effects, but data indicate that they protect the plants by induced resistance as evidenced from increased activity of β-1,3-glucanase.

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Abbreviations

A1:

Cation-exchanger A column

C1:

Anion-exchanger C column

C2:

Eluted anion-exchanger C column

NCF:

Non-charged fraction

GF:

Gel-filtration

dai:

Days after inoculation

SEM:

Standard error of the mean

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Acknowledgments

This project was funded by the Danish International Development Agency (Danida), Denmark, through the project 104.DAN.8.L.727 ‘Integrated disease and nutrient management in rice production systems’.

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Authors and Affiliations

  1. Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, DK-1871, Frederiksberg C, Denmark

    Julián Rodríguez-Algaba, Nguyễn Đắc Khoa, David B. Collinge & Hans Jørgen Lyngs Jørgensen

  2. Department of Food Science, Faculty of Science, University of Copenhagen, Rolighedsvej 30, DK-1958, Frederiksberg C, Denmark

    Jens Christian Sørensen & Hilmer Sørensen

  3. Department of Agroecology, Aarhus University, Forsøgsvej 1, DK-4200, Slagelse, Denmark

    Julián Rodríguez-Algaba

  4. Department of Molecular Biotechnology, Institute of Biotechnology, Research and Development, Can Tho University, 3/2 Street, Ninh Kieu District, Can Tho City, Vietnam

    Nguyễn Đắc Khoa

Authors
  1. Julián Rodríguez-Algaba
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  2. Jens Christian Sørensen
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  5. David B. Collinge
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  6. Hans Jørgen Lyngs Jørgensen
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Correspondence to Julián Rodríguez-Algaba or Hans Jørgen Lyngs Jørgensen.

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Rodríguez-Algaba, J., Sørensen, J.C., Sørensen, H. et al. Activity-guided separation of Chromolaena odorata leaf extract reveals fractions with rice disease-reducing properties. Eur J Plant Pathol 143, 331–341 (2015). https://doi.org/10.1007/s10658-015-0684-x

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  • DOI: https://doi.org/10.1007/s10658-015-0684-x

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