Abstract
Synergistic effects between urea and urease-positive bacteria against root-knot nematodes were assayed in laboratory and greenhouse trials. We isolated 85 urease-positive bacterial strains from soil, compost, sludge, and animal faeces; they belonged to four clades and sixteen genera of Eubacteria based on 16S rRNA BLAST. Twenty-five representative strains were assessed for nematicidal and urease activities. The urease-positive bacteria were nematicidal. A cubic relationship with a critical threshold was found between a strain’s nematicidal and urease activities. Sixteen strains with urease activity above this threshold had a 100 % nematicidal activity level. These 16 strains and urea alone were assessed for urease activity in pot experiments with tomato, which found that high-dose urea was nematicidal to root-knot nematodes (400–800 mg per kg soil) and phytotoxic (1,600 mg), while low-dose urea (≤200 mg) was non-nematicidal. However, nematodes could be controlled at 200 mg urea per kg soil in combination with urease-positive bacteria from seven different strains, and the nematode control efficacy of urea was always non-linearly positively correlated with soil urease activity. To test bacterial persistence, soils were recycled for another experiment, in which nine strains were significantly nematicidal, with a stronger relationship between nematode control efficacy and soil urease activity. These results suggest ways to manipulate soil bacteria to improve soil urease activity for the management of plant-parasitic nematodes. While urease-positive bacteria may have practical applications in controlling nematodes, their use will require careful field investigation and appropriate environmental safety evaluation.
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The authors wish to thank Louis and Jianrong Wu for their assistance with our English expression. This work was financially supported by grants from the “National Key Sciences and Technology Program for Water Solutions” (2012ZX07102-003), NSFC (30970100, 31160376), China.
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Wen, F., Zhang, Z., He, Y. et al. Synergism between urea and urease-positive bacteria in controlling root-knot nematodes. Eur J Plant Pathol 141, 179–191 (2015). https://doi.org/10.1007/s10658-014-0536-0
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DOI: https://doi.org/10.1007/s10658-014-0536-0