Abstract
A member of plant growth-promoting rhizobacteria, Bacillus megaterium YMF3.25, was demonstrated to be an efficient biocontrol agent (BCA) against root-knot nematode Meloidogyne incognita. Results from three-compartmented Petri dish tests and a pot experiment indicated that the bacterial culture could significantly inhibit the hatch of eggs and reduce infection of the nematode through production of nematicidal volatiles. After analysis by gas chromatograph/mass spectrometer and confirmation with commercial pure compounds, the nematicidal volatiles produced by the bacterium were characterised to include mainly the benzeneacetaldehyde, 2-nonanone, decanal, 2-undecanone and dimethyl disulphide, which were active against to both juveniles and eggs at the concentration of 0.5 mmol. Six compounds (phenyl ethanone, nonane, phenol, 3,5-dimethoxy-toluene, 2,3-dimethyl- butanedinitrile and 1-ethenyl-4-methoxy- benzene) with nematicidal activityies of 30%–63% also contributed to nematicidal efficacy of the bacterium.
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References
Ali, N. I., Siddiqui, I. A., Shaukat, S. S., & Zaki, M. J. (2002). Nematicidal activity of some strains of Pseudomonas spp. Soil Biology and Biochemistry, 34, 1051–1058.
Al-Rehiayani, S., Hafez, S. L., & Thornton, M. (1999). Effects of Pratylenchus neglectus, Bacillus megaterium, and oil radish or rapeseed green manure on reproductive potential of Meloidogyne chitwoodi on potato. Nematropica, 29(1), 37–49.
Butt, T. M., Jackson, C. W., & Magan, N. (2001). Fungi as biocontrol agents: progress, problems and potential. UK: CABI Publishing.
Campos, V. P., Souza, J. T., & Souza, R. G. (1998). Controle de Fitonematóides por Meio de Bactérias. Revisão Annual de Fitopatologia, 6, 285–327.
Chen, Z. X., & Dickson, D. W. (1998). Review of Pasteuria penetrans: biology, ecology, and biological control potential. Journal of Nematology, 30, 313–340.
Davies, K. G., Kerry, B. R., & Flynn, C. A. (1988). Observations on the pathogenicity of Pasteuria penetrans, a parasite of root-knot nematodes. Annals of Applied Biology, 112, 1491–1501.
De Freitas, J. R., Banerjee, M. R., & Germida, J. J. (1997). Phosphate-solubilizing rhizobacteria enhance the growth and yield but not phosphorus uptake of canola ( Brassica napus L.). Biology and Fertility of Soils, 24, 358–364.
Díaz, A., Vàzquez, L., Ventura, F., & Galceran, M. T. (2004). Estimation of measurement uncertainty for the determination of nonylphenol in water using solid-phase extraction and solid-phase microextraction procedures. Analytica Chimica Acta, 506, 71–80.
Gu, Y. Q., Zhou, J. P., Zou, C. S., Mo, M. H., & Zhang, K. Q. (2007). Evaluation and identification of potential organic nematocidal volatiles from soil bacteria. Soil Biology and Biochemistry, 39, 2567–2575.
Hussey, R. S., & Barker, K. (1973). R. A comparison of methods of collecting inocula of Meloidogyne spp. including a new technique. Plant Disease Reporter, 57, 1025–1028.
Javed, N., El-Hassan, S., Gowen, S., Pemproke, B., & Inam-ul-Hap, M. (2008). The potential of combining Pasteuria penetrans and neem (Azadirachta indica) formulation as a management system for root-knot nematodes on tomato. European Journal of Plant Pathology, 120, 53–60.
Liu, X. Z., Zhang, K. Q., & Li, T. F. (2004). Biological control of plant parasitic nematodes (In Chinese). Beijing, China: China Science & Technology Press.
López-Bucio, J., Campos-Cuevas, J. C., Hernández-Calderón, E., Velásquez-Becerra, C., Farías- Rodríguez, R., Macías-Rodríguez, L. I., et al. (2007). Bacillus megaterium rhizobacteria promote growth and alter root-system architecture through an Auxin- and Ethylene-independent signaling mechanism in Arabidopsis thaliana. Molecular Plant-Microbe Interactions, 20(2), 207–217.
Neipp, P. W., & Becker, J. O. (1999). Evaluation of biocontrol activity of rhizobacteria from Beta vulgaris against Heterodera schachtii. Journal of nematology, 31(1), 54–61.
Oliveira, D. F., Campos, V. P., Amaral, D. R., Nunes, A. S., Pantaleaõ, J. A., & Costa, D. A. (2007). Selection of rhizobacteria able to produce metabolites active against Meloidogyne exigua. European Journal of Plant Pathology, 119, 477–479.
Ramazan, C., Figen, D., Adil, A., & Fikrettin, S. (2006). Growth promotion of plants by plant growth-promoting rhizobacteria under greenhouse and two different field soil conditions. Soil Biology and Biochemistry, 38, 1482–1487.
Ristaino, J., & Thomas, W. (1998). Agriculture, methyl bromide and the ozone hole: can we fill the gap? Plant Disease, 81, 964–997.
Roberts, P. A. (1992). Current status of the availability, development and use of host plant resistance to nematodes. Journal of Nematology, 24, 213–227.
Sasser, J. N., & Freckman, D. W. (1987). A world perspective on nematology: the role of the society. In J. A. Veech, & D. W., Dickson (Eds.), Vistas on Nematology (pp. 7–14). Society of Nematologists, Hyatsville.
Wuest, P. J., & Bloom, J. R. (1965). Effect of temperature and age of egg population on the in vitro hatching of Meloidogyne hapla eggs. Phytopathology, 55, 885–888.
Xu, C. K., Mo, M. H., & Zhang, K. Q. (2004). Soil volatile fungistasis and volatile fungistatic compounds. Soil Biology and Biochemistry, 36, 1997–2004.
Zeng, D., Chen, B., Yao, S. Z., & Ying, J. Y. (2006). Determination of tetramethylenedisulfotetramine in human urine with gas chromatograph-flame thermionic detection coupling with direct immersed solidphase micro-extraction. Forensic Science International, 159, 168–174.
Acknowledgements
This work was supported jointly by the National Key Sciences and Technology Programme for Water Solutions (2008ZX07102-004), NSFC (30970100, 30760002) and projects from the Department of Science and Technology of Yunnan Province (2008GA012, 2006C0005M).
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Ying Huang and ChuanKun Xu contributed equally to this work.
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Huang, Y., Xu, C., Ma, L. et al. Characterisation of volatiles produced from Bacillus megaterium YFM3.25 and their nematicidal activity against Meloidogyne incognita . Eur J Plant Pathol 126, 417–422 (2010). https://doi.org/10.1007/s10658-009-9550-z
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DOI: https://doi.org/10.1007/s10658-009-9550-z