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Genetic population structure of polychaeta Neanthes glandicincta (Nereididae) of the Mai Po Inner Deep Bay Ramsar Site, Hong Kong

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Abstract

Neanthes glandicincta (Nereididae, Polychaeta) is the first numerically dominant benthic infauna in the Mai Po international Ramsar site, Hong Kong and also an economically important species for food source of birds and fishes. In present study, highly conserved nuclear ribosomal DNA (SSU and LSU rDNA) and mitochondrial COI gene were employed to study the population structure of N. glandicincta in the subtropical mudflat. The specimens were collected from five localities in February 2006, February–August 2007 and preserved at −80 °C, methanol or formalin, respectively. DNA extraction efficiency was the highest in fresh materials and lowest in formalin-fixed samples. The 18S (1774 bp), 28S D1 (383 bp) and COI genes were sequenced and analyzed. Both 18S and 28S D1 rDNA were highly conserved and showed no difference among the populations, whereas COI gene exhibited relatively high-level intraspecific polymorphism (2.2 %). The population from onshore and near mangrove station was phylogenetic different from other sites, indicating restricted gene exchange between the region of river mouth and mangrove forest. The mangrove may form a barrier for the dispersal of pelagic/benthic larvae of the population, which indicates that the population genetic difference is related to different habitats.

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Acknowledgments

This study was supported by the Ecological Monitoring Program for the Mai Po and Inner Deep Bay Ramsar Site under the contact No. AFCD/SQ/28/01-07, the Knowledge Innovation Program of the Chinese Academy of Sciences under contract SQ201202 and National Natural Science Foundation of China under contract 41006092 and 41130855. We would like to thank our team members in the Laboratory of Environmental Toxicology, the University of Hong Kong and the field working group for logistic support on sampling transport and assistance.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China

    Ping-Ping Shen

  2. Laboratory of Environmental Toxicology, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Pokfulam, Hong Kong

    Ji-Dong Gu

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  1. Ping-Ping Shen
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Correspondence to Ping-Ping Shen.

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Shen, PP., Gu, JD. Genetic population structure of polychaeta Neanthes glandicincta (Nereididae) of the Mai Po Inner Deep Bay Ramsar Site, Hong Kong. Ecotoxicology 24, 1557–1565 (2015). https://doi.org/10.1007/s10646-015-1465-1

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