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Significance of IL-1RA Polymorphism in Iranian Patients with Inflammatory Bowel Disease

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Abstract

Background and Aim

Interleukin (IL)-1 family members play an important role in the pathogenesis of inflammatory bowel disease (IBD). There are conflicting results regarding the association of IL-1 gene cluster single nucleotide polymorphisms (SNPs) with IBD and its clinical features. The aim of this study was to examine IL-1α −889 C/T, IL-1β −511 C/T, IL-1β +3962 C/T, IL-1R Pst-I1970 C/T, and IL-1RA Mspa-I11100 C/T SNPs in Iranian patients.

Methods

In this study, SNPs of IL-1 family members were investigated in 75 patients with IBD (40 CD and 35 UC), using polymerase chain reaction with sequence-specific primers method.

Results

IL-1β −511 CC genotype was significantly less present in UC compared to controls, while IL-1RA Mspa-I11100 CC was significantly associated with both Crohn’s disease (CD) and ulcerative colitis (UC). IL-1α −889 TT genotype was more frequently associated with extraintestinal manifestations. A significant association was observed between IL-1β +3962 TT genotype and the disease activity in IBD. IL-1RA Mspa-I11100 CC was significantly less frequent in CD patients who need immunosuppressive therapy. IL-1RA Mspa-I11100 CT was associated with earlier age of onset in IBD, while TT genotype was associated with higher age of onset in IBD.

Conclusions

IL-1 SNPs seem to be associated with IBD and could affect the disease severity as well.

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Acknowledgments

This study was supported by a grant from Tehran University of Medical Sciences (18104).

Conflict of interest

None.

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Authors and Affiliations

  1. Department of Gastroenterology and Hepatology, Tehran University of Medical Sciences, Tehran, Iran

    Nasser Ebrahimi Daryani

  2. Molecular Immunology Research Center, Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Maryam Sadr, Sepideh Shahkarami, Samaneh Soltani & Nima Rezaei

  3. Digestive Oncology Research Center, Digestive Disease Research Institute, Tehran University of Medical Sciences, Tehran, Iran

    Shirin Moossavi

  4. Hematology Department, School of Allied Medical Science, Iran University of Medical Sciences, Tehran, Iran

    Elham Farhadi

  5. Research Center for Immunodeficiencies, Children’s Medical Center Hospital, Tehran University of Medical Sciences, Dr Qarib St, Keshavarz Blvd, 14194, Tehran, Iran

    Nima Rezaei

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  1. Nasser Ebrahimi Daryani
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  2. Maryam Sadr
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Correspondence to Nima Rezaei.

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Daryani, N.E., Sadr, M., Moossavi, S. et al. Significance of IL-1RA Polymorphism in Iranian Patients with Inflammatory Bowel Disease. Dig Dis Sci 60, 1389–1395 (2015). https://doi.org/10.1007/s10620-014-3457-z

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  • DOI: https://doi.org/10.1007/s10620-014-3457-z

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