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A new method using insert-based systems (IBS) to improve cell behavior study on flexible and rigid biomaterials

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Abstract

In vitro studies about biomaterials biological properties are essential screening tests. Yet cell cultures encounter difficulties related to cell retention on material surface or to the observation of both faces of permeable materials. The objective of the present study was to develop a reliable in vitro method to study cell behavior on rigid and flexible/permeable biomaterials elaborating two specific insert-based systems (IBS-R and IBS-F respectively). IBS-R was designed as a specific cylindrical polytetrafluoroethylene (PTFE) system to evaluate attachment, proliferation and morphology of human gingival fibroblasts (HGFs) on grade V titanium and lithium disilicate glass-ceramic discs characteristics of dental prostheses. The number of cells, their covering on discs and their morphology were determined from MTS assays and microscopic fluorescent images after 24, 48 and 72 h. IBS-F was developed as a two components system to study HGFs behavior on guided bone regeneration polyester membranes. The viability and the membrane barrier effect were evaluated by metabolic MTS assays and by scanning electron microscopy. IBS-R and IBS-F were shown to promote (1) easy and rapid handling; (2) cell retention on biomaterial surface; (3) accurate evaluation of the cellular proliferation, spreading and viability; (4) use of non-toxic material. Moreover IBS-F allowed the study of the cell migration through degradable membranes, with an access to both faces of the biomaterial and to the bottom of culture wells for medium changing.

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Acknowledgments

We would like to express our thanks to Inion Ltd (Tampere, Finland), Kensey Nash (Eschborn, Germany), Nobel Biocare (Göteborg, Sweden), and Ivoclar Vivadent (Schaan, Liechtenstein) for sample supply. We would also like to thank Sirris (Liège, Belgium) for their assistance in the development of PTFE inserts.

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  1. Nicolas Moniotte

    Present address: GlaxoSmithKline Vaccines, Parc de la Noire Epine, Rue Fleming, 20, 1300–, Wavre, Belgium

Authors and Affiliations

  1. Dental Biomaterials Research Unit (d-BRU) and Department of Fixed Prosthodontics, Institute of Dentistry, University of Liège (ULg) and University of Liège Hospital (CHU), Quai Godefroid Kurth 45, 4020, Liège, Belgium

    Charlotte Grenade, Alain Vanheusden & Amélie Mainjot

  2. Mammalian Cell Culture Laboratory, GIGA-R, University of Liège (ULg), Liège, Belgium

    Nicolas Moniotte & Marie-Claire De Pauw-Gillet

  3. Department of Periodontology and Oral Surgery, Dental Biomaterials Research Unit (d-BRU), Institute of Dentistry, University of Liège (ULg) and University of Liège Hospital (CHU), Liège, Belgium

    Eric Rompen

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  1. Charlotte Grenade
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Correspondence to Charlotte Grenade.

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Grenade, C., Moniotte, N., Rompen, E. et al. A new method using insert-based systems (IBS) to improve cell behavior study on flexible and rigid biomaterials. Cytotechnology 68, 2437–2448 (2016). https://doi.org/10.1007/s10616-016-9964-3

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  • DOI: https://doi.org/10.1007/s10616-016-9964-3

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