Abstract
An enzymatic route to synthesize lutein esters was investigated using a lipase-Pluronic conjugate as the catalyst. For the synthesis of lutein palmitate, the enzymatic process gave a conversion of 85 % for lutein and a product purity of 96 % at 50 °C within 12 h, whereas the free lipase catalyzed process achieved a conversion below 10 %. To demonstrate the generality of this enzymatic process, lutein laurate, lutein myristate and lutein stearate were also synthesized achieving conversions of 73.8, 88.5, 84.4 % respectively. Moreover, the lipase-Pluronic conjugate can be reused for batches in the synthesis of lutein esters. As demonstrated by this study, the enzyme-Pluronic conjugate holds great promise for practical applications in industrial biocatalysis.
Graphical Abstract
An enzymatic approach to synthesize lutein esters was investigated using a lipase-Pluronic conjugate as the catalyst.
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Acknowledgments
This work was supported by the National High Technology Research and Development Program (“863” Program) of China under the Grant Number of 2014AA020507, the National Natural Science Foundation of China under the Grant Number of 21206082, Tsinghua University Initiative Scientific Research Program under the Grant Number of 20131089191.
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Hou, M., Wang, R., Wu, X. et al. Synthesis of Lutein Esters by Using a Reusable Lipase-Pluronic Conjugate as the Catalyst. Catal Lett 145, 1825–1829 (2015). https://doi.org/10.1007/s10562-015-1597-1
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DOI: https://doi.org/10.1007/s10562-015-1597-1