Neural prosthetic interfaces based upon penetrating microelectrode devices have broadened our understanding of the brain and have shown promise for restoring neurological functions lost to disease, stroke, or injury. However, the eventual viability of such devices for use in the treatment of neurological dysfunction may be ultimately constrained by the intrinsic brittleness of silicon, the material most commonly used for manufacture of penetrating microelectrodes. This brittleness creates predisposition for catastrophic fracture, which may adversely affect the reliability and safety of such devices, due to potential for fragmentation within the brain. Herein, we report the development of titanium-based penetrating microelectrodes that seek to address this potential future limitation. Titanium provides advantage relative to silicon due to its superior fracture toughness, which affords potential for creation of robust devices that are resistant to catastrophic failure. Realization of these devices is enabled by recently developed techniques which provide opportunity for fabrication of high-aspect-ratio micromechanical structures in bulk titanium substrates. Details are presented regarding the design, fabrication, mechanical testing, in vitro functional characterization, and preliminary in vivo testing of devices intended for acute recording in rat auditory cortex and thalamus, both independently and simultaneously.
KeywordsMEMS Prosthesis Electrophysiology Recording Microstimulation Brain-machine
Penetrating microelectrode devices, which allow direct and selective recording and stimulation of neural tissues, have provided unprecedented opportunity for enhancing understanding of neurophysiological processes underlying many aspects of human function and behavior (Bak et al. 1990, Gelbard-Sagiv et al. 2008). They have also shown immense promise for restoring neurological functions lost to disease, stroke, or injury (Normann 2007), as exemplified by neural prostheses that have enabled restoration of rudimentary auditory perception (Otto et al. 2008, Otto, Rousche and Kipke 2005a, Otto, Rousche and Kipke 2005b) and control of assistive instrumentation for those with motor dysfunction (Hochberg et al. 2006). However, this technology is still in its formative stages and many challenges remain.
While the improvement of chronic recording reliability remains a key near-term challenge for this emerging technology (Vetter et al. 2004), its eventual maturation into a clinically viable technique for restoring lost neurological function will also require development of devices that are capable of withstanding surgical manipulation by neurosurgeons (Kipke et al. 2008), and are immune to safety hazards associated with fracture-based fragmentation. The intrinsic brittleness of silicon, the material most commonly used for the manufacture of such devices, may ultimately prove limiting in this regard. This brittleness, which arises from the low fracture toughness of silicon, creates non-negligible probability for catastrophic failure by fracture. This has not been a significant concern in the evolution of this technology thus far. However, the potential for device fragmentation within the brain, no matter how small, coupled with the potentially severe consequences associated with such fragmentation in future clinical settings, suggests need for consideration of means for addressing this potential concern.
In addition to potential safety concerns, propensity for fracture of silicon-based microelectrodes may also adversely affect reliability, since fracture often results in complete loss of device functionality. It also places practical constraint on many current silicon devices with regard to the device length that can be reliably inserted, since risk of buckling-related fracture increases with device length, while opportunity for increasing buckling resistance through increased shank cross section area is constrained by potential adverse affects associated with increased insertion damage (Szarowski et al. 2003, Seymour and Kipke 2007). In many animal models, this practical length limitation hampers access to sub-cortical structures and largely precludes extension towards simultaneous recording within precisely-defined cortical and sub-cortical regions. This latter capability is of particular interest, since it may serve as a critical enabler for enhancing understanding of important neural processing networks, such as the corticothalamic loops that underlie auditory, visual, and somatosensory processing (Nicolelis et al. 1995, Fanselow and Nicolelis 1999, Sherman and Guillery 2002, Winer and Larue 1987, Hubel and N. 1972, Nicolelis and Shuler 2001). This, therefore, provides additional impetus to develop penetrating microelectrode devices with greater robustness.
Alternative microelectrode materials systems that have been explored include those based on ceramics (Moxon et al. 2004b), polymers (Lee et al. 2004a, Schuettler et al. 2005, Stieglitz et al. 2000, Rousche et al. 2001), and metals (Motta and Judy 2005, Fofonoff et al. 2004). Ceramics offer only limited benefit relative to silicon, with regard to reliability and safety, since they suffer from similar or greater propensity for fracture (Ward et al. 2009). Recently reported microelectrodes based on polycrystalline diamond show potential for realization of highly flexible, thin cross section devices with sufficient stiffness for cortical penetration (Chan et al. 2009). However, low signal to noise ratio observed during neural recording indicates need for further device refinement. Polymers used thus far possess sufficient toughness to mitigate fracture. However, their low elastic moduli typically require trade-off between device stiffness and functional reliability, since relatively large cross section areas are required to ensure insertion reliability and recording site placement accuracy (Lee et al. 2004b, Suzuki, Mabuchi and Takeuchi 2003, Takeuchi et al. 2004), which increases insertion damage. A number of strategies have been proposed to circumvent this trade-off, including those based on stiffening through incorporation of dissolvable rigid polymer coatings (Suzuki et al. 2003) or internal reinforcements (Lee et al. 2004b, Takeuchi et al. 2005, Takeuchi et al. 2004).
Metals show particular promise for use in microelectrode applications due to their high fracture toughness, which ensures graceful failure via plastic deformation, rather than catastrophic fracture (i.e. devices will bend before they break). This increases safety by minimizing potential for fragmentation, and may also enhance reliability, since potential exists for maintaining device functionality despite overloading. Moreover, when coupled with the moderate to high moduli of metals, this enables realization of robust microelectrodes with small cross section areas, thus facilitating insertion and reducing injury. However, despite these advantageous characteristics, metallic microelectrodes reported to-date suffer from limitations that may constrain their ultimate safety or utility. For example, in the devices demonstrated by Motta and Judy (Motta and Judy 2005), gold coatings were implemented to prevent exposure of the underlying nickel structural backbone to the physiological environment. This may adversely affect safety, due to potential for cytotoxic Ni ion release in the event of coating failure. In the devices demonstrated by Fofonoff et al. (Fofonoff et al. 2004), toxicity concerns were largely alleviated through the use of titanium as a structural backbone. However, opportunity for probing sequentially interconnected neural processing networks with such devices is constrained by reliance upon the Utah Electrode Array geometry, which consists of arrayed electrodes with only single, tip-located recording sites.
We have recently reported preliminary results from the development of titanium-based penetrating microelectrodes that seek to address the above-mentioned limitations (McCarthy et al. 2009). Realization of these devices was fundamentally enabled by recently developed techniques that allow, for the first time, fabrication of high-aspect-ratio micromechanical devices within bulk titanium substrates (Aimi et al. 2004, Parker et al. 2005). Herein, we expand upon this report to detail the design, fabrication, mechanical testing, and in vitro functional characterization of titanium-based microelectrode devices intended for use in acute rat auditory cortex and thalamus recording, both independently and simultaneously. Results from acute in vivo characterization of these devices are also briefly touched upon, but are more fully elaborated elsewhere (McCarthy, Rao and Otto 2011).
2 Design and fabrication
The microelectrodes produced in this effort were designed to study auditory cortex and thalamus responses within a rat model, both individually and simultaneously. As such, short devices (2 mm shank length) were designed for acute cortical recording, and longer devices (4.9 & 5.4 mm shank lengths) were designed for acute thalamic recording, independently of, or in conjunction with cortex. Both device types were modeled after commercially-available “Michigan” type electrode arrays (A1 × 16–5 mm 100–413, NeuroNexus Technologies, Ann Arbor, MI; Shank length = 5 mm, shank thickness ~ 15 μm, shank width = 33–200 μm (distal & proximal ends of shank, respectively), recording site diameter = 23 μm, & recording site pitch = 100 μm). Each titanium device contained 16 independently addressable circular recording sites, and 18 distinct design variants were developed with differing shank length (2, 4.9, & 5.4 mm), recording site diameter (23 & 40 μm), and pitch (50, 75, 100, & 150 μm). Since optimal designs for the intended applications have yet to be defined, the given variation of these parameters was reflective of the desire to eventually determine the most favorable combination that ensures consistent sampling of discrete neurons within the targeted neural regions with high signal to noise ratio.
Following metal deposition, the samples were soaked in acetone to release the Ti foils from the carrier wafer and then solvent cleaned. The second dielectric layer, composed of 0.2 μm Si3N4 followed by 0.8 μm of SiO2, was then deposited by PECVD to provide insulation from the surrounding environment (Benchmark 800 CVD, Process conditions Si3N4: 400 mT chamber pressure, 100 W RF power, 100 sccm NH3, 120 sccm 5% SiH4, and 300°C lower electrode temperature). This dual layer stack was chosen to promote adhesion to the underlying Au patterns and minimize stress-induced curvature of the devices arising from intrinsic stresses and thermal expansion mismatch between the deposited film and the underlying Ti substrate.
After completion of the second dielectric layer deposition, the Ti foils were again mounted to 100 mm carrier wafers with thermally conductive tape. The contact and recording site windows were then opened via photolithographic patterning and dry etching of the second dielectric layer (E620 R&D, Panasonic Factory Solutions, Japan; Process conditions: 7.5 mT chamber pressure, 500 W ICP source power, 400 W substrate RF power, and 40.0 sccm CHF3). The photoresist was then stripped and the samples were soaked in acetone to release the Ti foils from the carriers.
The microelectrode devices were packaged by bonding to commercially-available printed circuit boards (PCBs) (A-16, NeuroNexus Technologies) using cyanoacrylate adhesive (Pacer Technology, Rancho Cucamonga, CA). Gold wire-bonding was then used to make connections between the contact pads on the devices and their respective bond pads on the PCBs (7400A, West-Bond, Anaheim, CA). Afterwards, an additional layer of cyanoacrylate was applied over the contact pad area as an encapsulant to protect the exposed wires.
2.4 In situ recording site cleaning
3.1 Critical buckling force measurement
The buckling behavior of the microelectrode devices was assessed by longitudinal uniaxial compression testing. Both titanium devices and commercially-available silicon devices (A1 × 16–5 mm 100–413, NeuroNexus Technologies) were tested, the latter for performance benchmarking purposes. The devices were mounted to a silicon carrier chip using cyanoacrylate adhesive or double-sided carbon tape. The mounted devices were then attached to a manually-driven micromanipulator (M3301R, World Precision Instruments Inc., Sarasota, FL), which was used to load the device tips against a microbalance scale (AB54-S/FACT, Mettler Toledo, Columbus, OH). Forces exerted by the tips of the devices during testing were recorded using the microbalance (Rousche et al. 2001) and the devices were carefully observed for buckling and fracture via a CCD with magnifying optics. Five microelectrodes of the same design variant were tested for each length (i.e. Variant 3-L = 2 mm; Variant 9-L = 4.9 mm; and Variant 18-L = 5.4 mm).
3.2 In vitro functional characterization
Functional characterization was first performed with the microelectrode tip positioned well above the floor of the glass beaker to establish baseline device performance (i.e. Measurement #1–Unloaded, undeformed state). Afterwards, the harness holding the PCB was manually lowered until the microelectrode tip came into contact with the bottom of the beaker, thereby imposing longitudinal uniaxial compression. The harness was then lowered further until elastic buckling was observed, at which point the harness position was fixed and EIS and CV measurements were taken again (i.e. Measurement #2–Loaded, elastically buckled state). The harness was then further lowered until plastic deformation of the devices was induced (as verified by permanent deformation after unloading). Afterwards, the harness was raised sufficiently to fully unload the plastically-deformed device, and further EIS and CV measurements were made (i.e. Measurement #3–Unloaded, plastically deformed state). Two to three samples of each device length were tested.
3.3 In vivo recording
Preliminary acute in vivo recordings were performed in a rat model. As described in greater detail elsewhere (McCarthy et al. 2011), a male Long-Evans rat was chosen, weighed, and placed under anesthesia. A midline incision was made through the scalp and the muscles were retracted. A head holder mount was constructed to stabilize the rat’s skull during subsequent microelectrode insertion and testing. The skull over the primary auditory cortex of the right hemisphere was then drilled using a burr and the exposed area was cleaned and prepared for device insertion. The microelectrode array was inserted through the intact dura mater into the cortical mantle (i.e. dura was not retracted). Broadband acoustic noise stimuli were then administered and electrophysiological recording was performed through the connection of an external pre-amplifier (Tucker-Davis Technologies, Alachua, FL) connected to the microelectrode PCB. The pre-amplifier digitized and transmitted the neural signals to a neural recording system for amplification, analysis, and storage. All animal procedures complied with guidelines for the care and use of laboratory animals and were approved by the Purdue Animal Care and Use Committee.
4.1 Critical buckling force testing results
To simplify calculation of the theoretical critical bucking loads reported in Fig. 8, the tapered device shanks were approximated as uniform columns, i.e. columns with constant modulus, thickness, and width. The effective shank width was defined to be that which was necessary to maintain equivalent planar shank area for a given shank length. The shank thickness was defined as the sum of the thicknesses of the Ti foil used for the device fabrication and the dielectric layer stack (~ 1.5 μm). Although vendor specifications for the Ti foil indicated 25.4 μm nominal thickness, scanning electron microscope measurements performed on selected devices indicated actual thicknesses ranging from 34.2 to 37.6 μm with an average thickness of 35.2 μm (including dielectric stack), which slightly exceeds the stated manufacturing tolerance. As such, the experimentally measured average thickness was used for critical buckling load estimates. Finally, the elastic modulus of the column material was simply taken to be that of titanium, since the contribution of the dielectric layer stack would be minimized by its small thickness relative to the Ti substrate, and the relatively small modulus mismatch between Ti and the stack’s major constituents (i.e. ESiO2 = 70 GPa (Beer, Johnston and DeWolf 2002) and EAu = 80 GPa (Campbell 2001)). Although not reported here, finite element analyses performed for selected design variants with true device dimensions demonstrated excellent agreement with analytical solutions, thus suggesting that the underlying simplifying assumptions used for the analytical solutions did not introduce significant error.
4.2 In vitro functional characterization results
Measured EIS results for the titanium devices in the unloaded, undeformed state (i.e. measurements made with the device suspended in the in vitro characterization apparatus prior to mechanical loading against the beaker floor) indicated impedance values between 0.20 and 0.43 MΩ at 1 kHz frequency for recording site diameters of 40 μm, while a range of 0.75–1.38 MΩ was found for recording site diameters of 23 μm. Measured CV results revealed maximum charge carrying capacities ranging from 0.1 to 1.9 mC/cm2 for all design variants.
Average measured charge carrying capacities and impedances of 23 μm recording sites during buckling testing of titanium microelectrodes (10 sites over 2 devices)
Charge Carrying Capacity (mC/cm2)
0.32 ± 0.10
1.51 ± 0.23
Loaded, Elastically Deformed
0.60 ± 0.22
1.83 ± 0.53
Unloaded, Plastically Deformed
0.53 ± 0.28
1.67 ± 0.51
4.3 In vivo recording results
5.1 Buckling behavior
Assessment of buckling behavior was performed with the primary intent of ensuring that the titanium microelectrodes possessed sufficient stiffness for penetration of rat cortex, prior to undertaking in vivo studies. This was confirmed by observation of buckling loads for all device design variants (range = 12.26–132.35 mN) that were well in excess of the ~ 1 mN insertion force reportedly required for insertion of silicon microelectrodes with comparable design into rat cortex (Jensen, Yoshida and Hofmann 2006). This, therefore, suggests that the titanium microelectrodes will be able to sustain insertion forces many times greater than those expected in vivo.
As can be seen, the critical buckling loads for titanium and silicon-based devices can be related through the ratio of their elastic moduli. Substitution of appropriate moduli values into Eq. 3 reveals that the predicted critical buckling load for a titanium microelectrode would be approximately 66% of a comparable silicon device.
While reduced buckling resistance implies reduced insertion performance relative to silicon, the observation of graceful failure via plastic deformation indicates potential for additional load bearing capacity beyond the onset of elastic buckling, thus forestalling fracture, often significantly. This is evidenced in the current study, as well a prior study of titanium microneedles with similar geometry (Parker et al. 2007), by the absence of fracture-based failure in any titanium device, even those tested well beyond the elastic buckling limit. The intrinsic brittleness of silicon, in contrast, largely precludes plasticity, thus resulting in onset of fracture soon after the elastic buckling limit has been exceeded, as was observed during testing of the silicon devices in the current study. As such, titanium microelectrodes provide greater tolerance to overloading during insertion and largely preclude potential for catastrophic fragmentation within the brain, thus increasing potential for enhanced reliability and safety relative to conventional silicon devices.
The data presented in Fig. 8 suggest that neither fixed-pinned nor fixed-free end conditions accurately model the experimentally measured buckling loads for the titanium microelectrode devices, although there is slight bias towards the latter. This may be partly explained by potential for minor slippage between the device tip and the smooth surface of the silicon chip (RA < 1 nm RMS) and/or the chip and the microbalance plate. While there were no observations of gross tip slippage during testing, the limited spatial resolution of the imaging system may have precluded observation of less pronounced slippage. Furthermore, manual control of device displacement during testing reduced the accuracy with which the critical buckling load could be determined, thus introducing potential for additional error. However, despite these limitations, the measured data demonstrate that the analytical solutions (and their underlying simplifying assumptions) can be used to estimate the bounds within which buckling is expected, at least within the design space considered in the current study.
5.2 In vitro recording behavior
Assessment of electrical performance was primarily performed with the intent of ensuring that the titanium devices provided sufficient recording performance, prior to undertaking in vivo studies. This was confirmed by measurement of impedances for devices in the unloaded, undeformed state (40 μm diameter site range = 0.20–0.43 MΩ; 23 μm diameter site range = 0.75–1.38 MΩ) that were in good agreement with specified values for silicon-based commercial devices (range = 0.5–3.0 MΩ, depending on the recording site diameter (Paralikar and Clement 2008)). Similarly, measured charge carrying capacities (range = 0.1–1.9 mC/cm2) were observed to be in fair agreement with silicon devices reported in the literature which utilize gold recording sites of similar sizes (Wise et al. 2004).
A secondary intent of the in vitro characterization studies was to determine whether titanium microelectrodes were able to maintain recording functionality after being subjected to elastic buckling or plastic deformation. The inability to accurately measure either load or displacement during these studies precluded systematic and quantitative analysis of the effect of deformation on recording performance. However, the data shown in Fig. 9 qualitatively demonstrate that titanium microelectrodes are able to retain a significant portion of their original recording functionality during elastic buckling, as well as after plastic deformation and unloading. Silicon devices were not tested in these studies, but it is reasonable to expect that their brittleness would severely limit possibility for continued recording functionality beyond elastic buckling, since fracture would soon follow. As such, this suggests that titanium microelectrodes may provide greater recording reliability relative to silicon devices, since some level of recording functionality can be retained despite overloading.
Definitive elucidation of the mechanisms underlying the degradation of recording performance with device deformation is beyond the scope of the current study. However, the scanning electron micrographs shown in Fig. 10 suggest that low dielectric fracture toughness results in accumulation of damage within the dielectric stack, e.g. cracking or delamination. Concomitant damage to the gold traces and recording sites would also be expected, e.g. plasticity, cracking, and/or delamination from the dielectric at severe strains. These types of damage would be expected to have increasingly detrimental effect on the recording performance. Although the observation of increased impedance with deformation supports this presumption, further study is required for validation. Nevertheless, when coupled with the plastic failure mode of the underlying titanium shank, the observed retention of the damaged dielectric suggests potential for an added degree of safety during insertion, relative to silicon-based devices. The degree of deformation required to produce such damage would be immediately apparent during the insertion attempt and would most likely occur prior to actual penetration. As such, opportunity would be provided for unloading and removal of the damaged device with minimal danger of fragmentation or debris generation, thus minimizing potential for adverse effect upon surrounding neural tissue, aside from trauma associated with compressive dimpling of the cortical surface during the attempted insertion. This stands in contrast to brittle silicon-based devices, whose failure under buckling presents potential for generation of numerous atomically-sharp fragments, both large and small.
Finally, although less likely, it is conceivable that titanium-based devices could be subjected to mechanical overloading once inserted. In such cases, dielectric cracking could result in eventual debris generation within the brain, due to potential for delamination produced by penetration of physiological fluids into the cracks. While the magnitude and severity of such debris would be far less than that associated with fracture of a comparable silicon-based device, this represents a potential concern nonetheless. Replacement of the current brittle dielectrics with ductile polymers could provide one means for addressing this concern. Polyimide represents an attractive candidate in this regard, due to its demonstrated biocompatibility in neural implantation applications (Richardson, Miller and Reichert 1993, Stieglitz and Meyer 1999, Rubehn and Stieglitz 2010). The use of polyimide as the structural basis for neural prostheses (Rousche et al. 2001, Stieglitz and Gross 2001), as well as flexible cabling for such devices (Hetke, Najafi and Wise 1990), has been demonstrated. However, the coupling of polyimide with titanium (as the primary structural component) would offer means for improving insertion reliability and recording site placement accuracy while minimizing shank cross section area.
5.3 In vivo recording
Assessment of in vivo performance was primarily performed with the intent of validating cortical penetration capability of the titanium microelectrodes, as well their acute neural recording capability. The preliminary in vivo results confirmed that titanium microelectrodes possessed sufficient stiffness to penetrate not only rat pia, but also dura, which is typically retracted prior to insertion of commercially-available silicon devices. Moreover, initial in vivo studies generated isolatable action potentials with signal to noise ratios comparable to commercially-available silicon-based devices. Subsequent studies have also demonstrated capability for simultaneous acute recording from both auditory cortex and thalamus from the same microelectrode (McCarthy et al. 2011). To the best of our knowledge, this represents the first such measurement made using a single microelectrode device. As discussed earlier, this capability may provide significant opportunity for facilitating studies targeted towards enhancing understanding of sequentially interconnected neural processing networks.
While these results are promising, chronic implantation studies are needed to assess long-term device stability and recording reliability. Such studies may highlight need for consideration of additional or alternative dielectric materials, such as amorphous silicon carbide and silicon oxycarbide, which have been shown to provide superior chemical stability and impermeability relative to SiO2 and Si3N4 in physiological saline environments (Cogan et al. 2003). They may also demonstrate need for consideration of recording site coatings, such as sputtered iridium oxide, which have been shown to provide significantly lower impedance than noble metals (Cogan, Plante and Ehrlich 2004). Finally, and perhaps most importantly, such studies are also likely to demonstrate need for integration of means for mitigating reactive tissue response (i.e. gliosis), which often degrades recording quality with time (Williams, Rennaker and Kipke 1999, Vetter et al. 2004, Williams et al. 2007). Use of titanium rather than silicon as the structural backbone for microelectrode devices is unlikely to provide benefit in this regard, since such substitution does not address the presumed mechanisms underlying reactive tissue response, i.e. implantation-induced injury and/or mechanical impedance mismatch with the surrounding tissue. However, numerous strategies have been proposed within this context, including pharmacological intervention (Shain et al. 2003, Kim and Martin 2006, Zhong and Bellamkonda 2007), surface modification (Wadhwa, Lagenaur and Cui 2006, Moxon et al. 2004a, Abidian and Martin 2009), and novel device design (Seymour and Kipke 2007, Kipke et al. 2008, Muthuswamy et al. 2005). The unique capabilities afforded by titanium micromachining, coupled with the favorable properties of titanium, may prove particularly advantageous for implementation of such strategies.
We have reported the design, fabrication, mechanical testing, in vitro, and in vivo characterization of titanium-based, “Michigan” type, penetrating microelectrodes for acute neural recording in rat. Mechanical testing results demonstrate that these devices posses sufficient stiffness for reliable cortical penetration, and also demonstrate that they provide potential for greater mechanical reliability and safety relative to current commercially-available silicon devices, due to their graceful, plasticity-based failure mode. In vitro characterization studies demonstrate that the titanium devices provide recording performance comparable to that of silicon-based devices, and also demonstrate that they provide potential for greater recording reliability, since plasticity-based failure allows maintenance of a significant degree of recording functionality despite overloading. Finally, results from preliminary in vivo studies corroborate many of these findings by demonstrating capability for reliable cortical insertion and acute recording with good signal to noise. Collectively, these results demonstrate the potential embodied in titanium as a promising new structural material for penetrating microelectrodes.
The authors would like to thank the Center for Neural Communication Technology for providing the silicon microelectrodes (NIH/NIBIB P41-EB002030). The authors also thank the staff at the Birck Nanotechnology Center (Purdue University) for assistance in device fabrication, and R. Madangopal and the students of the NeuroProstheses Research Laboratory (Purdue University) for assistance with in vitro characterization. Finally, the authors acknowledge support provided by the Cordier Fellowship Program (School of Mechanical Engineering, Purdue University), the Purdue Research Foundation, and the Showalter Research Trust (Purdue University).
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