Article

Biomedical Microdevices

, Volume 12, Issue 5, pp 761-767

Open Access This content is freely available online to anyone, anywhere at any time.

Multiphysics simulation of a microfluidic perfusion chamber for brain slice physiology

  • Hector H. CaicedoAffiliated withDepartment of Bioengineering, University of Illinois at Chicago
  • , Maximiliano HernandezAffiliated withDepartment of Bioengineering, University of Illinois at Chicago
  • , Christopher P. FallAffiliated withDepartment of Bioengineering, University of Illinois at Chicago
  • , David T. EddingtonAffiliated withDepartment of Bioengineering, University of Illinois at Chicago Email author 

Abstract

Understanding and optimizing fluid flows through in vitro microfluidic perfusion systems is essential in mimicking in vivo conditions for biological research. In a previous study a microfluidic brain slice device (μBSD) was developed for microscale electrophysiology investigations. The device consisted of a standard perfusion chamber bonded to a polydimethylsiloxane (PDMS) microchannel substrate. Our objective in this study is to characterize the flows through the μBSD by using multiphysics simulations of injections into a pourous matrix to identify optimal spacing of ports. Three-dimensional computational fluid dynamic (CFD) simulations are performed with CFD-ACE + software to model, simulate, and assess the transport of soluble factors through the perfusion bath, the microchannels, and a material that mimics the porosity, permeability and tortuosity of brain tissue. Additionally, experimental soluble factor transport through a brain slice is predicted by and compared to simulated fluid flow in a volume that represents a porous matrix material. The computational results are validated with fluorescent dye experiments.

Keywords

Microfluidics Computational fluid dynamics (CFD) simulations Organotypic brain slice cultures (OBSC)