Biomedical Microdevices

, Volume 11, Issue 4, pp 903–913

A microfluidic cell culture platform for real-time cellular imaging

Article

DOI: 10.1007/s10544-009-9307-7

Cite this article as:
Hsieh, CC., Huang, SB., Wu, PC. et al. Biomed Microdevices (2009) 11: 903. doi:10.1007/s10544-009-9307-7

Abstract

This study reports a new microfluidic cell culture platform for real-time, in vitro microscopic observation and evaluation of cellular functions. Microheaters, a micro temperature sensor, and micropumps are integrated into the system to achieve a self-contained, perfusion-based, cell culture microenvironment. The key feature of the platform includes a unique, ultra-thin, culture chamber with a depth of 180 μm, allowing for real-time, high-resolution cellular imaging by combining bright field and fluorescent optics to visualize nanoparticle-cell/organelle interactions. The cell plating, culturing, harvesting and replenishing processes are performed automatically. The developed platform also enables drug screening and real-time, in situ investigation of the cellular and sub-cellular delivery process of nano vectors. The mitotic activity and the interaction between cells and the nano drug carriers (conjugated quantum dots-epirubicin) are successfully monitored in this device. This developed system could be a promising platform for a wide variety of applications such as high-throughput, cell-based studies and as a diagnostic cellular imaging system.

Keywords

MicrofluidicsMicro-bioreactorsCell cultureNanoparticleCellular imagingMEMS

Nomenclature

Au

gold

CCD

Charge-coupled device

DOF

Depth of field

EMV

Electromagnetic valve

FBS

Fetal bovine serum

HEPES

4-(2-hydroxyethyl)-1-piperazineethanesulfonic Acid

ITO

Indium-tin-oxide

MTT

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium Bromide

OC-2

Oral cancer cell

PDMS

Polydimethylsiloxane

Pt

Platinum

QD

Quantum dots

S-shape

Serpentine-shape

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Department of Engineering ScienceNational Cheng Kung UniversityTainanTaiwan
  2. 2.Institute of Oral Medicine and Department of Stomatology and Institute of Innovation and Advanced StudiesNational Cheng Kung UniversityTainanTaiwan
  3. 3.Institute of Basic Medical SciencesNational Cheng Kung UniversityTainanTaiwan
  4. 4.Medical Electronics and Device Technology CenterIndustrial Technology Research InstituteHsinchuTaiwan