Biomedical Microdevices

, Volume 11, Issue 4, pp 903–913

A microfluidic cell culture platform for real-time cellular imaging

Authors

  • Chia-Chun Hsieh
    • Department of Engineering ScienceNational Cheng Kung University
  • Song-Bin Huang
    • Department of Engineering ScienceNational Cheng Kung University
  • Ping-Ching Wu
    • Institute of Basic Medical SciencesNational Cheng Kung University
    • Institute of Oral Medicine and Department of Stomatology and Institute of Innovation and Advanced StudiesNational Cheng Kung University
    • Department of Engineering ScienceNational Cheng Kung University
    • Medical Electronics and Device Technology CenterIndustrial Technology Research Institute
Article

DOI: 10.1007/s10544-009-9307-7

Cite this article as:
Hsieh, C., Huang, S., Wu, P. et al. Biomed Microdevices (2009) 11: 903. doi:10.1007/s10544-009-9307-7

Abstract

This study reports a new microfluidic cell culture platform for real-time, in vitro microscopic observation and evaluation of cellular functions. Microheaters, a micro temperature sensor, and micropumps are integrated into the system to achieve a self-contained, perfusion-based, cell culture microenvironment. The key feature of the platform includes a unique, ultra-thin, culture chamber with a depth of 180 μm, allowing for real-time, high-resolution cellular imaging by combining bright field and fluorescent optics to visualize nanoparticle-cell/organelle interactions. The cell plating, culturing, harvesting and replenishing processes are performed automatically. The developed platform also enables drug screening and real-time, in situ investigation of the cellular and sub-cellular delivery process of nano vectors. The mitotic activity and the interaction between cells and the nano drug carriers (conjugated quantum dots-epirubicin) are successfully monitored in this device. This developed system could be a promising platform for a wide variety of applications such as high-throughput, cell-based studies and as a diagnostic cellular imaging system.

Keywords

Microfluidics Micro-bioreactors Cell culture Nanoparticle Cellular imaging MEMS

Nomenclature

Au

gold

CCD

Charge-coupled device

DOF

Depth of field

EMV

Electromagnetic valve

FBS

Fetal bovine serum

HEPES

4-(2-hydroxyethyl)-1-piperazineethanesulfonic Acid

ITO

Indium-tin-oxide

MTT

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium Bromide

OC-2

Oral cancer cell

PDMS

Polydimethylsiloxane

Pt

Platinum

QD

Quantum dots

S-shape

Serpentine-shape

Copyright information

© Springer Science+Business Media, LLC 2009