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Spatial and developmental expression of key genes of terpene biosynthesis in Tanacetum parthenium

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Biologia Plantarum

Abstract

Feverfew (Tanacetum parthenium) is a medicinal plant belonging to the Asteraceae family. To improve understanding terpene metabolism in feverfew, the relative gene expression of four key genes coding 3-hydroxy-3-methylglutarylcoenzyme A reductase (HMGR) and germacrene A synthase (GAS) from the mevalonic acid pathway (MVA), as well as 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) and hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase (HDR) from the methyl erythritol phosphate pathway (MEP), were examined. Target organs and tissues included young leaves (not fully expanded), mature leaves (fully expanded), flowers, stems, roots, and glandular trichomes. HMGR, DXR, and HDR were isolated and sequenced for the first time in feverfew. Real-time quantitative PCR analysis revealed differential expression of these genes in feverfew tissues and developmental stages.

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Abbreviations

DMADP:

dimethylallyl diphosphate

DXP:

1-deoxy-D-xylulose-5-phosphate

DXR:

1-deoxy-D-xylulose- 5-phosphate reductoisomerase

FDP:

farnesyl diphosphate

GAS:

germacrene A synthase

HDR:

hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase

HMGR:

3-hydroxy-3-methylglutaryl-coenzyme A reductase

IDP:

isopentenyl diphosphate

MEP:

methylerythritol phosphate

MVA:

mevalonic acid

ORF:

open reading frame

RACE:

rapid amplification of cDNA ends

RGE:

relative gene expression

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Authors and Affiliations

  1. Department of Agricultural Biotechnology, University of Kurdistan, Sanandaj, 6617715175, Iran

    M. Majdi

  2. Plant Breeding and Biotechnology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, 1411713111, Iran

    G. Karimzadeh

  3. National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, 1497716316, Iran

    M. A. Malboobi

  4. Research Center for Medicinal Plant Breeding and Development, Faculty of Agriculture, University of Kurdistan, Sanandaj, 6617715175, Iran

    M. Majdi

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  1. M. Majdi
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  2. G. Karimzadeh
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  3. M. A. Malboobi
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Correspondence to M. Majdi.

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Acknowledgements: This work was financially supported by the University of Kurdistan and the Iran National Science Foundation. We gratefully thank Claudia Vickers (the Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Australia) for reading the manuscript and useful comments. Jafar Abdollahzadeh (the Department of Plant Protection, Faculty of Agriculture, University of Kurdistan, Iran) is acknowledged for phylogenetic analysis.

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Majdi, M., Karimzadeh, G. & Malboobi, M.A. Spatial and developmental expression of key genes of terpene biosynthesis in Tanacetum parthenium . Biol Plant 58, 379–384 (2014). https://doi.org/10.1007/s10535-014-0398-5

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  • DOI: https://doi.org/10.1007/s10535-014-0398-5

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