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Cu/Zn superoxide dismutase activity and respective gene expression during cold acclimation and freezing stress in barley cultivars

  • Published:
Biologia Plantarum

Abstract

The transcript levels and activities of the superoxide dismutase isoenzyme (Cu/ZnSOD) were assessed in winter (Tarm-92) and spring (Zafer-160) barley cultivars during cold acclimation, freezing stress and after rewarming. Leaf Cu/ZnSOD activity and Cu/ZnSOD expression level were not significantly changed during cold acclimation. The Cu/ZnSOD expression increased evidently at mild freezing stress (−3 °C; F1), while Cu/ZnSOD1 activity did not show any response and Cu/ZnSOD2 activity decreased continuously during F1 and F2 (−7 °C) in Tarm-92. On the other hand, root Cu/ZnSOD2 activity was in accordance with Cu/ZnSOD expression in Zafer-160 after F2 treatment. Rewarming periods did not cause any significant changes in the Cu/ZnSOD activity and expression of Cu/ZnSOD in both cultivars when compared to freezing stresses. These results showed that freezing stress can regulate differently Cu/ZnSOD transcription and enzyme activity.

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Abbreviations

APX:

ascorbate peroxidase

CA:

cold-acclimated

CAT:

catalase

GR:

glutathione reductase

NBT:

nitroblue tetrazolium

ROS:

reactive oxygen species

RT-PCR:

reverse transcription polymerase chain reaction

SOD:

superoxide dismutase

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Authors and Affiliations

  1. Department of Biology, Middle East Technical University, 06531, Ankara, Turkey

    C. Kayihan, N. Afsar, H. A. Oktem & M. Yucel

  2. Faculty of Education, Baskent University, 06810, Ankara, Turkey

    F. Eyidogan

Authors
  1. C. Kayihan
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  2. F. Eyidogan
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  3. N. Afsar
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  4. H. A. Oktem
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  5. M. Yucel
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Corresponding author

Correspondence to F. Eyidogan.

Additional information

Acknowledgements: We would like to thank Turkish Ministry of Agriculture and Rural Affairs for barley cultivars Tarm-92 and Zafer-160. This study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK TOVAG-105O152).

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Kayihan, C., Eyidogan, F., Afsar, N. et al. Cu/Zn superoxide dismutase activity and respective gene expression during cold acclimation and freezing stress in barley cultivars. Biol Plant 56, 693–698 (2012). https://doi.org/10.1007/s10535-012-0143-x

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  • DOI: https://doi.org/10.1007/s10535-012-0143-x

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