Abstract
Objectives
To identify and characterize a novel antimicrobial peptide, catesbeianin-1.
Results
Catesbeianin-1 is 25 amino acids long and is α-helical, cationic and amphipathic. It had antimicrobial activity against Gram-positive and Gram-negative bacteria. It was resistant against trypsin and pepsin. Catesbeianin-1 exhibited moderate hemolytic activity (approx 8%) at 100 μg/ml, and its HC50 (50% hemolytic concentration) was 300 μg/ml. Its cytotoxicity was approx 10–20% at 100 μg/ml, and its CC50 (50% cytotoxic concentration) was >100 μg/ml. The LD50 of catesbeianin-1 in mice was 80 mg/kg. At 3.1 µg/ml, catesbeianin-1 significantly inhibited the growth of methicillin-resistant Staphylococcus aureus.
Conclusions
A new antimicrobial peptide from the skin of Lithobates catesbeianus (American bullfrog) may represent a template for the development of novel antimicrobial agents.
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Acknowledgements
The authors thank Meiye Li, Li Liu, Fengguang Pan and Yan Zhang for generously providing the necessary experimental equipment. This research was supported by the Specialized Research Fund for the Doctoral Program of China (20130061130001).
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Supplementary Table 1—Strains of bacteria used.
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Xu, H., Zhang, Y., Feng, X. et al. Catesbeianin-1, a novel antimicrobial peptide isolated from the skin of Lithobates catesbeianus (American bullfrog). Biotechnol Lett 39, 897–903 (2017). https://doi.org/10.1007/s10529-017-2312-7
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DOI: https://doi.org/10.1007/s10529-017-2312-7