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Expression of lycopene biosynthesis genes fused in line with Shine-Dalgarno sequences improves the stress-tolerance of Lactococcus lactis

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Abstract

Objectives

Lycopene biosynthetic genes from Deinococcus radiodurans were co-expressed in Lactococcus lactis to produce lycopene and improve its tolerance to stress.

Results

Lycopene-related genes from D. radiodurans, DR1395 (crtE), DR0862 (crtB), and DR0861 (crtI), were fused in line with S hine-Dalgarno (SD) sequences and co-expressed in L. lactis. The recombinant strain produced 0.36 mg lycopene g-1 dry cell wt after 48 h fermentation. The survival rate to UV irradiation of the recombinant strain was higher than that of the non-transformed strain.

Conclusion

The L. lactis with co-expressed genes responsible for lycopene biosynthesis from D. radiodurans produced lycopene and exhibited increased resistance to UV stress, suggesting that the recombinant strain has important application potential in food industry.

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Acknowledgments

This work was supported by the Special Fund for Provincial Key Technologies R & D Program of Henan (Grant Nos 152102110045 and 152102310064) and the Henan base and cutting-edge technology research projects (162300410131).

Supporting information

Supplementary Table 1—The primer sequences used.

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Authors and Affiliations

  1. Henan Key Laboratory of Ion-beam Bioengineering, College of Physics and Engineering, Zhengzhou University, Zhengzhou, 450001, China

    Xiangrong Dong, Yanping Wang, Fengyuan Yang & Shanshan Zhao

  2. Key Laboratory for Nuclear-Agricultural Sciences of Chinese Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China

    Bing Tian & Tao Li

Authors
  1. Xiangrong Dong
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  2. Yanping Wang
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  3. Fengyuan Yang
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  4. Shanshan Zhao
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  5. Bing Tian
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  6. Tao Li
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Correspondence to Yanping Wang.

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The authors declare that they have no conflict of interest.

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Dong, X., Wang, Y., Yang, F. et al. Expression of lycopene biosynthesis genes fused in line with Shine-Dalgarno sequences improves the stress-tolerance of Lactococcus lactis . Biotechnol Lett 39, 65–70 (2017). https://doi.org/10.1007/s10529-016-2220-2

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  • DOI: https://doi.org/10.1007/s10529-016-2220-2

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