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Rapid evolution of hyaluronan synthase to improve hyaluronan production and molecular mass in Bacillus subtilis

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Abstract

Objectives

To improve the production and molecular mass of the glycosaminoglycan hyaluronan (HA) in Bacillus subtilis by engineering hyaluronan synthase (HAS) from Streptococcus zooepidemicus.

Results

By mutating regions within HAS intracellular domains, five positive variants exhibiting higher HA production (from 1.22 to 2.24 g l−1) and molecular mass values (from 1.20 to 1.36 × 106 Da) were constructed and characterized. Overexpression of the V5 variant and the genes tuaD and glmU increased HA production and molecular mass to 2.8 g l−1 and 2.4 × 106 Da, respectively.

Conclusions

This study provides a novel strategy for improving HA production and its molecular mass.

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Acknowledgments

This work was financially supported by a grant from the Key Technologies R & D Program of Jiangsu Province, China (BE2014607), the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT_15R26) and the 111 Project.

Supporting information

Supplementary Table 1—Plasmids and primers used.

Author information

Authors and Affiliations

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China

    Linpei Zhang, Hao Huang, Hao Wang, Jian Chen, Guocheng Du & Zhen Kang

  2. The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China

    Guocheng Du & Zhen Kang

  3. Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, 214122, Jiangsu, China

    Jian Chen, Guocheng Du & Zhen Kang

Authors
  1. Linpei Zhang
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  2. Hao Huang
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  3. Hao Wang
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  4. Jian Chen
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  5. Guocheng Du
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  6. Zhen Kang
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Corresponding author

Correspondence to Zhen Kang.

Electronic supplementary material

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Supplementary material 1 (DOCX 12 kb)

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Zhang, L., Huang, H., Wang, H. et al. Rapid evolution of hyaluronan synthase to improve hyaluronan production and molecular mass in Bacillus subtilis . Biotechnol Lett 38, 2103–2108 (2016). https://doi.org/10.1007/s10529-016-2193-1

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  • DOI: https://doi.org/10.1007/s10529-016-2193-1

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