Abstract
Objectives
To improve 1,3-propanediol (1,3-PD) production and reduce byproduct concentration during the fermentation of Klebsiella pneumonia.
Results
Klebsiella. pneumonia 2-1ΔldhA, K. pneumonia 2-1ΔaldH and K. pneumonia 2-1ΔldhAΔaldH mutant strains were obtained through deletion of the ldhA gene encoding lactate dehydrogenase required for lactate synthesis and the aldH gene encoding acetaldehyde dehydrogenase involved in the synthesis of ethanol. After fed-batch fermentation, the production of 1,3-PD from glycerol was enhanced and the concentrations of byproducts were reduced compared with the original strain K. pneumonia 2-1. The maximum yields of 1,3-PD were 85.7, 82.5 and 87.5 g/l in the respective mutant strains.
Conclusion
Deletion of either aldH or ldhA promoted 1,3-PD production in K. pneumonia.
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Acknowledgments
This work was financially supported by a grant from Shandong Province Independent Innovation and Achievement Transformation Project (201422cx02602) and a grant from Taishan scholar Construction Project.
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Supplementary Table 1—Primers used in this study.
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Chen, L., Ma, C., Wang, R. et al. Deletion of ldhA and aldH genes in Klebsiella pneumoniae to enhance 1,3-propanediol production. Biotechnol Lett 38, 1769–1774 (2016). https://doi.org/10.1007/s10529-016-2155-7
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DOI: https://doi.org/10.1007/s10529-016-2155-7