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Dynamic cell culture on porous biopolymer microcarriers in a spinner flask for bone tissue engineering: a feasibility study

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Abstract

Porous microspherical carriers have great promise for cell culture and tissue engineering. Dynamic cultures enable more uniform cell population and effective differentiation than static cultures. Here we applied dynamic spinner flask culture for the loading and multiplication of cells onto porous biopolymer microcarriers. The abilities of the microcarriers to populate cells and to induce osteogenic differentiation were examined and the feasibility of in vivo delivery of the constructs was addressed. Over time, the porous microcarriers enabled cell adhesion and expansion under proper dynamic culture conditions. Osteogenic markers were substantially expressed by the dynamic cell cultures. The cell-cultured microcarriers implanted in the mouse subcutaneous tissue for 4 weeks showed excellent tissue compatibility, with minimal inflammatory signs and significant induction of bone tissues. This first report on dynamic culture of porous biopolymer microcarriers providing an effective tool for bone tissue engineering.

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Acknowledgment

This work was supported by a grant from Dankook University in 2013.

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Authors and Affiliations

  1. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, 330-714, Republic of Korea

    Guang-Zhen Jin, Jeong-Hui Park, Seog-Jin Seo & Hae-Won Kim

  2. Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, 330-714, Republic of Korea

    Jeong-Hui Park & Hae-Won Kim

  3. Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan, 330-714, Republic of Korea

    Hae-Won Kim

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  1. Guang-Zhen Jin
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  2. Jeong-Hui Park
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  4. Hae-Won Kim
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Correspondence to Hae-Won Kim.

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Jin, GZ., Park, JH., Seo, SJ. et al. Dynamic cell culture on porous biopolymer microcarriers in a spinner flask for bone tissue engineering: a feasibility study. Biotechnol Lett 36, 1539–1548 (2014). https://doi.org/10.1007/s10529-014-1513-6

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  • DOI: https://doi.org/10.1007/s10529-014-1513-6

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