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Intracellular production of IFN-alpha 2b in Lactococcus lactis

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Abstract

Human interferon alpha (IFN-α) was expressed in two strains of Lactococcus lactis by aid of two promoters (P32 and Pnis) giving rise to two recombinant strains: MG:IFN and NZ:IFN, respectively. The expression of IFN was confirmed by ELISA and western blotting. Highest production was achieved using glucose for growth of both recombinant strains with nisin, used for induction of the recombinant strain with Pnis promoter, at 30 ng/ml. The optimum time for MG:IFN was 9 h and for NZ:IFN was 4.5 h. The highest productions by MG:IFN and NZ:IFN were 1.9 and 2.4 μg IFN/l, respectively. Both of the expressed IFNs showed bioactivities of 1.9 × 106 IU/mg that were acceptable for further clinical studies.

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Acknowledgments

We would like to thank the Ministry of Science, Technology and Innovation, Malaysia and Malaysian Genome Institute (MGI) for funding and technical support during the study period. We also thank Kees Leenhouts for the kind gift of plasmids pNZ8048 and pMG36e, and L. lactis hosts NZ9000 and MG1363.

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Authors and Affiliations

  1. Department of Cell and Molecular Biology, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Omid Bayat, Ali Baradaran & Raha Abdul Rahim

  2. Department of Bioprocess Technology, Faculty of Biotechnology & Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Arbakariya Ariff & Rosfarizan Mohamad

  3. Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Raha Abdul Rahim

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  1. Omid Bayat
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  2. Ali Baradaran
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  3. Arbakariya Ariff
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  4. Rosfarizan Mohamad
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  5. Raha Abdul Rahim
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Correspondence to Raha Abdul Rahim.

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Bayat, O., Baradaran, A., Ariff, A. et al. Intracellular production of IFN-alpha 2b in Lactococcus lactis . Biotechnol Lett 36, 581–585 (2014). https://doi.org/10.1007/s10529-013-1390-4

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  • DOI: https://doi.org/10.1007/s10529-013-1390-4

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