Abstract
The gene encoding enterotoxigenic Escherichia coli B-subunit heat-labile toxin (LTB) antigen was co-transformed into hairy root cultures of Nicotiana tabacum (tobacco), Solanum lycopersicum (tomato) and Petunia parodii (petunia) under the CaMV35S promoter. Tobacco and petunia roots contained ~65–70 μg LTB g−1 tissue whilst hairy roots of tomato contained ~10 μg LTB g−1. Antigen at ~600 ng ml−1 was detected in growth medium of tobacco and petunia. Tobacco roots with higher LTB levels showed growth retardation of ~80% whereas petunia hairy roots with similar levels of LTB showed only ~35% growth retardation, relative to vector controls. Regeneration of plants from LTB-containing tobacco hairy roots was readily achieved and re-initiated hairy roots from greenhouse-grown plants showed similar growth and LTB production characteristics as the original hairy root cultures.
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Acknowledgments
We are grateful to Elena Virtue and Dr Raelene Pickering, for providing unpublished data and advice on hairy root culture; Dr Kathleen De Boer and Suzy Ryan for general technical advice and laboratory support. GDG acknowledges receipt of a graduate support scholarship from Monash University. This work was supported by Monash University support grants to JDH and DW and an Australian Research Council (ARC) Linkage grant involving AMW and JDH.
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De Guzman, G., Walmsley, A.M., Webster, D.E. et al. Hairy roots cultures from different Solanaceous species have varying capacities to produce E. coli B-subunit heat-labile toxin antigen. Biotechnol Lett 33, 2495–2502 (2011). https://doi.org/10.1007/s10529-011-0710-9
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DOI: https://doi.org/10.1007/s10529-011-0710-9