Abstract
Snake melon is an important cucurbit crop especially in the Southeastern and the Mediterranean region of Turkey. It is consumed as fresh or pickled. The production is mainly done with the local landraces in the country. Turkey is one of the secondary diversification centers of melon and possesses valuable genetic resources which have different morphological characteristics in case of snake melon. Genetic diversity of snake melon genotypes collected from different regions of Turkey and reference genotypes obtained from World Melon Gene Bank in Avignon-France was examined using 13 simple sequence repeat (SSR) markers. A total of 69 alleles were detected, with an average of 5.31 alleles per locus. The polymorphism information content of SSR markers ranged from 0.19 to 0.57 (average 0.38). Based on cluster analysis, two major groups were defined. The first major group included only one accession (61), while the rest of all accessions grouped in the second major group and separated into different sub-clusters. Based on SSR markers, cluster analysis indicated that considerably high genetic variability exists among the examined accessions; however, Turkish snake melon accessions were grouped together with the reference snake melon accessions.
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Aierken Y, Akashi Y, Phan TPN, Halidan Y, Tanaka K, Long B, Nishida H, Long CL, Wu MZ, Kato K (2011) Molecular analysis of the genetic diversity of Chinese Hami melon and its relationship to the melon germplasm from Central and South Asia. J Jpn Soc Hortic Sci 80:52–65
Ali-Shtayeh, Jamous RM, Shtaya MJ, Mallah OB, Eid IS, Abu Zaitoun SY (2015) Morphological characterization of snake melon (Cucumis melo var. flexuosus) populations from Palestine. Genet Resour Crop Evol. doi:10.1007/s10722-015-0329-0
Anonymous (2014) TUİK Turkish Statistical Institute, www.tuik.gov.tr
Besirli G, Yanmaz R (1997) Types of the snake cucumber (Cucumis melo var. flexuosus Naud.) Grown in the south east region of Turkey. In: Proceedings of the First International Symposium on Cucurbits, 20–23 May 1997, Adana Acta Hortic 492:37–40
Chiba N, Suwabe K, Nunome T, Hirai M (2003) Development of microsatellite markers in melon (Cucumis melo L.) and their application to major cucurbit crops. Breed Sci 53:21–27
Danin-Poleg Y, Reis N, Tzuri G, Katzir N (2001) Development and characterization of microsatellite markers in Cucumis. Theor Appl Genet 102:61–72
Edwards K, Johnstone C, Thompson C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res 19:1349
Escribano S, Lazaro A, Cuevas HE, Loopez-Sese AI, Staub JE (2012) Spanish melons (Cucumis melo L.) of the Madrid provenance: a unique germplasm reservoir. Genet Resour Crop Evol 59:359–373
Esposito MA, Martin EA, Cravero VP, Cointry E (2007) Characterization of pea accessions by SRAP’s markers. Sci Hort 113:329–335
Fazio G, Staub JE, Chung SM (2002) Development and characterization of PCR markers in cucumber (Cucumis sativus L.). J Am Soc Hortic Sci 127:545–557
Gonzalo MJ, Oliver M, Garcia-Mas J, Monforte A, Dolcet-Sanjuan R, Katzir N, Arús P, Monforte AJ (2005) Development of a consensus map of melon (Cucumis melo L.) based on high-quality markers (RFLPs and SSRs) using F2 and double-haploid line populations. Theor Appl Genet 110:802–811
Hu J, Wang P, Su Y, Wang R, Li Q, Sun K (2015) Microsatellite diversity, population structure, and core collection formation in melon germplasm. Plant Mol Biol Rep 33:439–447
Jaccard P (1908) Nouvelles sur la distribution florale. Bulletin de la Société Vaudoise des Sciences Naturelles 44:223–270
Jarne P, Lagoda PJL (1996) Microsatellites, from molecules to populations and back. Trends Ecol Evol 11:424–429
Kacar YA, Simsek O, Solmaz I, Sari N, Mendi YY (2012) Genetic diversity among melon accessions (Cucumis melo) from Turkey based on SSR markers. Genet Mol Res 11:622–4631
Kose MT (2008) Genetic and morphological characterization of Turkey’s snake melon (Cucumis melo var. flexuosus) M.Sc. Thesis University of Cukurova, Adana
Monforte AJ, Garcia-Mas J, Arus P (2003) Genetic variability in melon based on microsatellite variation. Plant Breed 122:153–157
Nantoume AD, Andersen SB, Jensen BD (2013) Genetic differentiation of watermelon landrace types in Mali revealed by microsatellite (SSR) markers. Gen Res Crop Evol 60:2129–2141
Ning X, Xiong L, Wang X, Gao X, Zhang Z, Zhong L, Li G (2014) Genetic diversity among Chinese Hami melon and its relationship with melon germplasm of diverse origins revealed by microsatellite markers. Bioch Syst and Ecol 57:432–438
Pandey S, Dhillon NPS, Sureja AK, Singh D, Malik AA (2010) Hybridization for increased yield and nutritional content of snake melon (Cucumis melo L. var. flexuosus). Plant Genet Resour 8:127–131
Pitrat M (2008) Melon. In: Prohens J, Nuez F (eds) Handbook of Plant Breeding, vol 1. Springer, New York, pp 283–315
Pitrat M, Hanelt P, Hammer K (2000) Some comments on infraspecific classification of cultivars of melon. Acta Hortic 510:29–36
Powell W, Machray GC, Provan J (1996) Polymorphism revealed by simple sequence repeats. Trends Plant Sci 1:215–222
Raghami ML, Lopez-Sese AI, Hasandokht MR, Zamani Z, Moghadam MRF, Kashi A (2014) Genetic diversity among melon accessions from Iran and their relationships with melon germplasm of diverse origins using microsatellite markers. Plant Syst Evol 300:139–151
Robinson RW, Decker-Walters D (1997) Cucurbits. Cab International, New York
Rohlf FJ (1998) NTSYS-PC Numerical Taxonomy and Multivariate Analysis System. Version 2.00. Exeter software, Setauket
Smith JSC, Chin ECL, Shu H, Smith OS, Wall SJ, Senior ML, Mitchel SE, Kresorich S, Tiegle J (1997) An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.): comparisons with data from RFLPs and pedigree. Theor Appl Genet 95:163–173
Solmaz I, Aras V, Unlu H, Sarı N (2004). Türkiye’nin farklı bölgelerinden toplanan acur (Cucumis melo var. flexuosus) genotiplerinde karakterizasyon. Turkiye V. Sebze Tarimi Sempozyumu Bildirileri 21–24 Eylul 2004 Canakkale, 75-81 (In Turkish)
Soltani F, Akashi Y, Kashi A, Zamani Z, Mostofi Y, Kato K (2010) Characterization of Iranian melon landraces of Cucumis melo L. Groups Flexuosus and Dudaim by analysis of morphological characters and random amplified polymorphic DNA. Breed Sci 60:34–45
Tzitzikas NE, Monforte AJ, Fatihi A, Kypriotakis Z, Iacovides TA, Ioannides IM, Kalaitzis P (2009) Genetic diversity and population structure of traditional Greek and Cypriot melon cultigens (Cucumis melo L.) based on simple sequence repeat variability. HortScience 44:1820–1824
Vural H, Esiyok D, Duman I (2000) Kultur Sebzeleri (Sebze Yetistirme) Ege Universitesi Ziraat Fakultesi Bahce Bitkileri Bolumu, Ege Universitesi Basımevi, Izmir (In Turkish)
Wang P, Li Q, Hu J, Su Y (2015) Comparative analysis of genetic diversity among Chinese watermelon germplasms using SSR and SRAP markers, and implications for future genetic improvement. Turk J Agric For 39:322–331
Yildiz M, Akgul N, Sensoy S (2014) Morphological and molecular characterization of Turkish landraces of Cucumis melo L. Not Bot Horti Agrobo 42:51–58
Acknowledgments
This research was supported by the Scientific Research Projects Coordination Unit of Cukurova University (FBA-2014-2380). The authors are grateful to Dr. Aydin Uzun for his kindly help in the PIC analysis; AARI, TAEA, EMTZARI, INRA (Michel Pitrat), and Veysel Aras for seed supplying.
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Solmaz, I., Kacar, Y.A., Simsek, O. et al. Genetic Characterization of Turkish Snake Melon (Cucumis melo L. subsp. melo flexuosus Group) Accessions Revealed by SSR Markers. Biochem Genet 54, 534–543 (2016). https://doi.org/10.1007/s10528-016-9739-8
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DOI: https://doi.org/10.1007/s10528-016-9739-8