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Construction of Full-Length cDNA Library and Development of EST-Derived Simple Sequence Repeat (EST-SSR) Markers in Senecio scandens

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Abstract

Senecio scandens Buch.-Ham. ex D. Don (Compositae) is a crucial source of Chinese traditional medicine with antibacterial properties. We constructed a cDNA library and obtained expressed sequence tags (ESTs) to show the distribution of gene ontology annotations for mRNAs, using an individual plant with superior antibacterial characteristics. Analysis of comparative genomics indicates that the putative uncharacterized proteins (21.07%) might be derived from “molecular function unknown” clones or rare transcripts. Furthermore, the Compositae had high cross-species transferability of EST-derived simple sequence repeats (EST-SSR), based on valid amplifications of 206 primer pairs developed from the newly assembled expressed sequence tag sequences in Artemisia annua L. Among those EST-SSR markers, 52 primers showed polymorphic amplifications between individuals with contrasting diverse antibacterial traits. Our sequence data and molecular markers will be cost-effective tools for further studies such as genome annotation, molecular breeding, and novel transcript profiles within Compositae species.

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Acknowledgments

The authors gratefully acknowledge the financial support of this project by the Special Foundation Administered by Supervisor in Guizhou province of China (QZH-2008-61).

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Authors and Affiliations

  1. Department of Cell Biology and Genetics, Zunyi Medical College, 201 Dalian Road, Zunyi, 563003, Guizhou, China

    Gang Qian, Junjiao Ping, Jian Lu, Zhen Zhang, Lei Wang & Delin Xu

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  1. Gang Qian
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  2. Junjiao Ping
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  3. Jian Lu
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  4. Zhen Zhang
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  5. Lei Wang
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  6. Delin Xu
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Correspondence to Gang Qian.

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Qian, G., Ping, J., Lu, J. et al. Construction of Full-Length cDNA Library and Development of EST-Derived Simple Sequence Repeat (EST-SSR) Markers in Senecio scandens . Biochem Genet 52, 494–508 (2014). https://doi.org/10.1007/s10528-014-9662-9

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  • DOI: https://doi.org/10.1007/s10528-014-9662-9

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