Skip to main content
SpringerLink
Log in

Combination of the biocontrol yeast Cryptococcus laurentii with UV-C treatment for control of postharvest diseases of tomato fruit

  • Published:
BioControl Aims and scope Submit manuscript

Abstract

Integrated management that combines different methods is being actively pursued in the control of postharvest disease. In this study, the biocontrol yeast Cryptococcus laurentii and ultraviolet-C (UV-C) treatment were evaluated for controlling infection following artificial inoculation with Botrytis cinerea or Alternaria alternata, and natural infection in tomato fruit. Applied separately, C. laurentii and UV-C (4 kJ m−2) effectively inhibited decay caused by B. cinerea or A. alternata, and natural infection. The combination of C. laurentii and UV-C showed better control efficiency. UV-C treatment did not affect yeast growth in fruit wounds, while the treatment induced the transcript expression of β-1,3-glucanase, phenylalanine ammonia-lyase, peroxidase and superoxide dismutase based on real-time PCR analysis, as well as increased the activity of these enzymes in tomato fruit. Results indicate that the mechanism by which UV-C enhanced the biocontrol efficacy of C. laurentii may be associated with the elicitation of defense response in tomato fruit.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Assis JS, Maldonado R, Muñoz T, Escribano MI, Merodio C (2001) Effect of high carbon dioxide concentration on PAL activity and phenolic contents in ripening cherimoya fruit. Postharvest Biol Technol 23:33–39

    Article  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  PubMed  CAS  Google Scholar 

  • Cao J, Jiang W (2006) Induction of resistance in Yali pear (Pyrus bretschneideri Rehd.) fruit against postharvest diseases by acibenzolar-S-methyl sprays on trees during fruit growth. Sci Hortic 110:181–186

    Article  CAS  Google Scholar 

  • Chan Z, Tian S (2006) Induction of H2O2-metabolizing enzymes and total protein synthesis by antagonistic yeast and salicylic acid in harvested sweet cherry fruit. Postharvest Biol Technol 39:314–320

    Article  CAS  Google Scholar 

  • Chand-Goyal T, Spotts RA (1997) Biological control of postharvest diseases of apple and pear under semi-commercial and commercial conditions using three saprophytic yeasts. Biol Control 10:199–206

    Article  Google Scholar 

  • Charles MT, Benhamou N, Arul J (2008a) Physiological basis of UV-C-induced resistance to Botrytis cinerea in tomato fruit: III. Ultrastructural modifications and their impact on fungal colonization. Postharvest Biol Technol 47:27–40

    Article  CAS  Google Scholar 

  • Charles MT, Goulet A, Arul J (2008b) Physiological basis of UV-C-induced resistance to Botrytis cinerea in tomato fruit: IV. Biochemical modification of structural barriers. Postharvest Biol Technol 47:41–53

    Article  CAS  Google Scholar 

  • Charles MT, Mercier J, Makhlouf J, Arul J (2008c) Physiological basis of UV-C-induced resistance to Botrytis cinerea in tomato fruit I. Role of pre- and post-challenge accumulation of the phytoalexin-rishitin. Postharvest Biol Technol 47:10–20

    Article  CAS  Google Scholar 

  • Charles MT, Tano K, Asselin A, Arul J (2009) Physiological basis of UV-C-induced resistance to Botrytis cinerea in tomato fruit. V. Constitutive defence enzymes and inducible pathogenesis-related proteins. Postharvest Biol Technol 51:414–424

    Article  CAS  Google Scholar 

  • Charles MT, Arul J, Benhamou N (2011) UV-C-induced disease resistance in tomato fruit is a multi-component and time-dependent system. Acta Hort 905:251–260

    CAS  Google Scholar 

  • Cia P, Benato EA, Pascholati SF (2010) Use of irradiation in postharvest disease management: problems and solutions. Stewart Postharvest Rev 6:1–7

    Article  Google Scholar 

  • Cota IE, Troncoso-Rojasa R, Sotelo-Mundo R, Sánchez-Estrada A, Tiznado-Hernández ME (2007) Chitinase and β-1,3-glucanase enzymatic activities in response to infection by Alternaria alternata evaluated in two stages of development in different tomato fruit varieties. Sci Hortic 112:42–50

    Article  CAS  Google Scholar 

  • de Capdeville G, Wilson CL, Beer SV, Aist JR (2002) Alternative disease control agents induce resistance to blue mold in harvested ‘red delicious’ apple fruit. Phytopathology 92:900–908

    Article  PubMed  Google Scholar 

  • Dixon RA, Achnin L, Kota P, Liu CJ, Srinivasa MS, Wang L (2002) The phenyl-propanoid pathway and plant defence - a genomics perspective. Mol Plant Pathol 3:371–390

    Article  PubMed  CAS  Google Scholar 

  • Droby S, Wisniewski M, Macarisin D, Wilson C (2009) Twenty years of postharvest biocontrol research: is it time for a new paradigm? Postharvest Biol Technol 52:137–145

    Article  Google Scholar 

  • El Ghaouth A, Wilson CL, Callahan AM (2003) Induction of chitinase, β-1,3-glucanase, and phenylalanine ammonia lyase in peach fruit by UV-C treatment. Phytopathology 93:349–355

    Article  PubMed  Google Scholar 

  • Erkan M, Wang SY, Wang CY (2008) Effect of UV treatment on antioxidant capacity, antioxidant enzyme activity and decay in strawberry fruit. Postharvest Biol Technol 48:163–171

    Article  CAS  Google Scholar 

  • González-Aguilar GA, Wang CY, Buta JG, Krizek DT (2001) Use of UV-C irradiation to prevent decay and maintain postharvest quality of ripe ‘Tommy Atkins’ mangoes. Int J Food Sci Technol 36:767–773

    Article  Google Scholar 

  • Ippolito A, El-Ghaouth A, Wilson CL, Wisniewski M (2000) Control of postharvest decay of apple fruit by Aureobasidium pullulans and induction of defense responses. Postharvest Biol Technol 19:265–272

    Article  CAS  Google Scholar 

  • Jamalizadeh M, Etebarian HR, Aminian H, Alizadeh A (2011) A review of mechanisms of action of biological control organisms against post-harvest fruit spoilage. EPPO Bull 41:65–71

    Article  Google Scholar 

  • Janisiewicz WJ, Conway WS (2010) Combining biological control with physical and chemical treatments to control fruit decay after harvest. Stewart Postharvest Rev 6:1–16

    Article  Google Scholar 

  • Janisiewicz WJ, Korsten L (2002) Biological control of postharvest diseases of fruits. Annu Rev Phytopathol 40:411–441

    Article  PubMed  CAS  Google Scholar 

  • Janisiewicz WJ, Saftner RA, Conway WS, Yoder KS (2008) Control of blue mold decay of apple during commercial controlled atmosphere storage with yeast antagonists and sodium bicarbonate. Postharvest Biol Technol 49:374–378

    Article  CAS  Google Scholar 

  • Jin P, Zheng Y, Tang S, Rui H, Wang CY (2009) Enhancing disease resistance in peach fruit with methyl jasmonate. J Sci Food Agr 89:802–808

    Article  CAS  Google Scholar 

  • Korsten (2006) Advances in control of postharvest diseases in tropical fresh produce. Int J Postharvest Technol Innov 1:48–61

  • Lamikanra O, Kueneman D, Ukuku D, Bett-Garber KL (2005) Effect of processing under ultraviolet light on the shelf life of fresh-cut cantaloupe melon. J Food Sci 70:534–539

    Article  Google Scholar 

  • Lee J, Bricker TM, Lefevre M, Pinson SRM, Oard JH (2006) Proteomic and genetic approaches to identifying defence-related proteins in rice challenged with the fungal pathogen Rhizoctonia solani. Mol Plant Pathol 7:405–416

    Article  PubMed  CAS  Google Scholar 

  • Li J, Zhang Q, Cui Y, Yan J, Cao J, Zhao Y, Jiang W (2010) Use of UV-C treatment to inhibit the microbial growth and maintain the quality of Yali pear. J Food Sci 75:503–507

    Article  Google Scholar 

  • Lima G, de Curtis F, Castoria R, de Cicco V (1998) Activity of the yeasts Cryptococcus laurentii and Rhodotorula glutinis against post-harvest rots on different fruits. Biocontrol Sci Technol 8:257–267

    Article  Google Scholar 

  • Liu C, Cai L, Han X, Ying T (2011) Temporary effect of postharvest UV-C irradiation on gene expression profile in tomato fruit. Gene 486:56–64

    Article  PubMed  CAS  Google Scholar 

  • Mari M, Guizzardi M, Brunelli M, Folchi A (1996) Postharvest biological control of grey mould (Botrytis cinerea Pers.: Fr.) on fresh-market tomatoes with Bacillus amyloliquefaciens. Crop Prot 15:699–705

    Article  Google Scholar 

  • Mclaughlin RJ, Wilson CL, Chalutz E, Kurtzman CP, Fett WF, Osman SF (1990) Characterization and reclassification of yeasts used for biological control of postharvest diseases of fruits and vegetables. Appl Environ Microbiol 56:3583–3586

    PubMed  CAS  Google Scholar 

  • Meng XH, Qin GZ, Tian SP (2010) Influences of preharvest spraying Cryptococcus laurentii combined with postharvest chitosan coating on postharvest diseases and quality of table grapes in storage. LWT-Food Sci Technol 43:596–601

    Article  CAS  Google Scholar 

  • Obande MA, Tucker GA, Shama G (2011) Effect of preharvest UV-C treatment of tomatoes (Solanum lycopersicon Mill.) on ripening and pathogen resistance. Postharvest Biol Technol 62:188–192

    Article  CAS  Google Scholar 

  • Polevaya Y, Alkalai-Tuvia S, Copel A, Fallik E (2002) Early detection of grey mould development in tomato after harvest. Postharvest Biol Technol 25:221–225

    Article  Google Scholar 

  • Qin GZ, Tian SP (2004) Biocontrol of postharvest diseases of jujube fruit by Cryptococcus laurentii combined with a low dosage of fungicides under different storage conditions. Plant Dis 88:497–501

    Article  Google Scholar 

  • Reeleder RD (2004) The use of yeasts for biological control of the plant pathogen Sclerotinia sclerotiorum. BioControl 49:583–594

    Article  Google Scholar 

  • Romanazzi G, Gabler FM, Smilanick JL (2006) Preharvest chitosan and postharvest UV irradiation treatments suppress gray mold of table grapes. Plant Dis 90:445–450

    Article  CAS  Google Scholar 

  • Shama G, Alderson P (2005) UV hormesis in fruits: a concept ripe for commercialisation. Trends Food Sci Technol 16:128–136

    Article  CAS  Google Scholar 

  • Sharma RR, Singh D, Singh R (2009) Biological control of postharvest diseases of fruits and vegetables by microbial antagonists: a review. Biol Control 50:205–221

    Article  Google Scholar 

  • Smilanick (2011) Integrated approaches to postharvest disease management in California citrus packinghouses. Acta Hort 905:145–148

  • Song W, Ma X, Tan H, Zhou J (2011) Abscisic acid enhances resistance to Alternaria solani in tomato seedlings. Plant Physiol Biochem 49:693–700

    Article  PubMed  CAS  Google Scholar 

  • Spadaro D, Gullino ML (2004) State of the art and future prospects of the biological control of postharvest fruit diseases. Int J Food Microbiol 91:185–194

    Article  PubMed  Google Scholar 

  • Stevens C, Khan VA, Lu JY, Wilson CL, Pusey PL, Igwegbe ECK, Kabwe MK, Mafolo Y, Liu J, Chalutz E, Droby S (1997) Integration of ultraviolet (UV-C) light with yeast treatment for control of postharvest storage rots of fruits and vegetables. Biol Control 10:98–103

    Article  Google Scholar 

  • Stevens C, Khan VA, Wilson CL, Lu JY, Chalutz E, Droby S (2005) The effect of fruit orientation of postharvest commodities following low dose ultraviolet light-C treatment on host induced resistance to decay. Crop Prot 24:756–759

    Article  Google Scholar 

  • Su H, Gubler WD (2012) Effect of 1-methylcyclopropene (1-MCP) on reducing postharvest decay in tomatoes (Solanum lycopersicum L.). Postharvest Biol Technol 64:133–137

    Article  CAS  Google Scholar 

  • Teixidó N, Usall J, Palou L, Asensio A, Nunes C, Viñas I (2001) Improving control of green and blue molds of oranges by combining Pantoea agglomerans (CPA-2) and sodium bicarbonate. Eur J Plant Pathol 107:685–694

    Article  Google Scholar 

  • Tian SP, Yao HJ, Deng X, Xu XB, Qin GZ, Chan ZL (2007) Characterization and expression of β-1,3-glucanase genes in jujube fruit induced by the microbial biocontrol agent Cryptococcus laurentii. Phytopathology 97:260–268

    Article  PubMed  CAS  Google Scholar 

  • Wang Y, Tian S, Xu Y, Qin G, Yao H (2004) Changes in the activities of pro- and anti-oxidant enzymes in peach fruit inoculated with Cryptococcus laurentii or Penicillium expansum at 0 or 20 °C. Postharvest Biol Technol 34:21–28

    Article  CAS  Google Scholar 

  • Wang F, Feng G, Chen K (2009) Defense responses of harvested tomato fruit to burdock fructooligosaccharide, a novel potential elicitor. Postharvest Biol Technol 52:110–116

    Article  CAS  Google Scholar 

  • Wang A, Lou B, Xu T, Lin C (2011) Defense responses in tomato fruit induced by oligandrin against Botrytis cinerea. Afr J Biotechnol 10:4596–4601

    CAS  Google Scholar 

  • Wisniewski M, Wilson C, Droby S, Chalutz E, El Ghaouth A, Stevens C (2007) Postharvest biocontrol: new concepts and applications. In: Vincent C, Goettal MS, Lazarovits G (eds) Biological control: a global perspective. CABI, Cambridge, UK, pp 262–273

    Chapter  Google Scholar 

  • Wisniewski M, Norelli J, Bassett C, Artlip T, Macarisin D (2011) Ectopic expression of a novel peach (Prunus persica) CBF transcription factor in apple (Malus × domestica) results in short-day induced dormancy and increased cold hardiness. Planta 233:917–983

    Article  Google Scholar 

  • Xu L, Du Y (2012) Effects of yeast antagonist in combination with UV-C treatment on postharvest diseases of pear fruit. BioControl 57:451–461

    Article  CAS  Google Scholar 

  • Xu XB, Tian SP (2008) Reducing oxidative stress in sweet cherry fruit by Pichia membranaefaciens: a possible mode of action against Penicillium expansum. J Appl Microbiol 105:1170–1177

    Article  PubMed  CAS  Google Scholar 

  • Yao H, Tian S (2005) Effect of pre- and post-harvest application of salicylic acid or methyl jasmonate on inducing disease resistance of sweet cherry fruit in storage. Postharvest Biol Technol 35:253–262

    Article  CAS  Google Scholar 

  • Zeng K, Deng Y, Ming J, Deng L (2010) Induction of disease resistance and ROS metabolism in navel oranges by chitosan. Sci Hortic 126:223–228

    Article  CAS  Google Scholar 

  • Zhang H, Zheng X, Xi Y (2005) Biological control of postharvest blue mold of oranges by Cryptococcus laurentii (Kufferath) Skinner. BioControl 50:331–342

    Article  Google Scholar 

  • Zhang H, Zheng X, Yu T (2007) Biological control of postharvest diseases of peach with Cryptococcus laurentii. Food Control 18:287–291

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by grants from the Ph. D Foundation of Shandong Institute of Commerce and Technology (2010.10), National High Technology Research and Development Program of China (2011AA100702) and National Technology R&D Program in the 12th Five Year Plan of China (2011BAD24B02).

Author information

Authors and Affiliations

  1. Shandong Key Laboratory of Storage and Transportation Technology of Agricultural Products, Shandong Institute of Commerce and Technology, 4516 Travel Road, Caishi, Licheng District, Jinan, 250103, Shandong, China

    Changfeng Zhang & Guoli Wang

  2. Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Huajiachi Campus, Hangzhou, 310029, China

    Changfeng Zhang & Kunsong Chen

  3. National Engineering Research Center for Agricultural Products Logistics, Jinan, 250103, China

    Changfeng Zhang & Guoli Wang

Authors
  1. Changfeng Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kunsong Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Guoli Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Changfeng Zhang.

Additional information

Handling Editor: Monica Höfte

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, C., Chen, K. & Wang, G. Combination of the biocontrol yeast Cryptococcus laurentii with UV-C treatment for control of postharvest diseases of tomato fruit. BioControl 58, 269–281 (2013). https://doi.org/10.1007/s10526-012-9477-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10526-012-9477-8

Keywords

Search

Navigation