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Some clinical, microbiological and molecular characteristics of Aeromonas hydrophila isolated from various naturally infected fishes

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Abstract

Heat-stable cytotonic enterotoxin gene (Ast) was detected by polymerase chain reaction (PCR) of twenty isolates of Aeromonas hydrophila isolated from various naturally infected fishes collected from both fresh and brackish water. These fishes were Nile tilapia and meagre, mullet and sea bream, respectively. Antibiotic susceptibility, pathogenic characteristics of these isolates and histopathological alterations of liver from experimentally infected tilapia fish with A. hydrophila which contained Ast gene were investigated. PCR technique for the detection of Ast as specific gene for A. hydrophila genomes showed that 90% of tested A. hydrophila (18/20) contained Ast gene, which is specific for A. hydrophila (SSU).The in vitro susceptibility of 18 strains of A. hydrophila (SSU) to 9 antibiotics was evaluated. Oxytetracycline only was an effective antibiotic for all tested isolates. On contrast, all these isolates were resistant to amoxicillin, ampicillin and penicillin. Pathogenicity assay in this study proved that 33.3% of the tested A. hydrophila (6/18) were pathogenic for tilapia in vitro with various levels of virulence where 2/6 were classified as strongly virulent according to the severity of mortality rate. Microscopically, A. hydrophila toxins apparently cause irreparable systemic damage to liver which leads to death.

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References

  • Abdo MH (2004) Seasonal variations of some heavy metals in macrophytes and water of Damietta branch, River Nile, Egypt. Egypt J Aquat Biol Fish 8:195–211

    Google Scholar 

  • Aguilera-Arreola MG, Hernandez-Rodrfguez C, Zuniga G, Figueras MJ, Castro-Escarpulli G (2005) Aeromonas hydrophila clinical and environmental ecotypes as revealed by genetic diversity and virulence genes. FEMS Microbiol Lett 242:231–240

    Article  CAS  PubMed  Google Scholar 

  • Albert MJ, Ansaruzzaman M, Talukder KA, Chopra AK, Kuhn I, Rahman M, Faruque AS, Islam MS, Sack RB, Mollby R (2000) Prevalence of enterotoxin genes in Aeromonas spp. isolated from children with diarrhea, healthy controls, and the environment. J Clin Microbiol 38:3785–3790

    CAS  PubMed  Google Scholar 

  • Ali A, Karunasagar I, Karunasagar I (1997) Effect of oxytetracycline on the immune response of Labaeo rohita to Aeromonas hydrophila vaccine. In: Flegel T, MacRae IH (eds) Diseases in Asian aquaculture 6 Vol. III. Fish Health Section, Asian Fisheries Society, Manila, Philippines, pp 187–191

    Google Scholar 

  • Altarriba M, Merino S, Gavin R, Canals R, Rabaan A, Shaw JG, Tomas JM (2003) A polar flagella operon (flg) of Aeromonas hydrophila contains genes required for lateral flagella expression. Microb Pathog 34:249–259

    Article  CAS  PubMed  Google Scholar 

  • Bizani D, Brandelli A (2001) Antimicrobial susceptibility, hemolysis, and hemagglutination among Aeromonas spp. Isolated from water of a bovine abattoir. Braz J Microbiol 32:334–339

    Article  CAS  Google Scholar 

  • Brenden RA, Huizinga HW (1986) Pathophysiology of experimental Aeromonas hydrophila infection in goldfish Carassius auratus (L.). J Fish Dis 9:163–167

    Article  Google Scholar 

  • Burke V, Robinose J, Atkinson HM, Gracey M (1982) Biochemical characteristics of enerotoxogenic Aeromonas sp. J Clin Microbiol 15:48–52

    CAS  PubMed  Google Scholar 

  • Cahill MM (1990) Virulence factors in motile Aeromonas species. J Appl Bacteriol 69:1–16

    CAS  PubMed  Google Scholar 

  • Cascón A, Anguita J, Hernanz C, Sánchez M, Fernández M, Naharro G (1996) Identification of Aeromonas hydrophila hybridization group 1 by PCR assays. Appl Environ Microbiol 62:1167–1170

    PubMed  Google Scholar 

  • Chang CY, Thompson H, Rodman N, Bylander J, Thomas J (1997) Pathogenic analysis of Aeromonas hydrophila septicemia. Ann Clin Lab Sci 27:254–259

    CAS  PubMed  Google Scholar 

  • Choo PS (1994) Degradation of oxytetracycline hydrochloride in fresh and sea water. Asian Fish Sci 7:195–200

    Google Scholar 

  • Del Corral F, Shotts EB Jr, Brown J (1990) Adherence, haemagglutination, and cell surface characteristics of motile aeromonads virulent for fish. J Fish Dis 13:255–268

    Article  Google Scholar 

  • Dixon BA (1994) Antibiotic resistance of bacterial fish pathogens. J World Aquac Soc 25:60–63

    Article  Google Scholar 

  • Emekdas G, Aslan G, Tezcan S, Serin SM, Yildiz C, Ozturhan H, Durmaz R (2006) Detection of the frequency, antimicrobial susceptibility, and genotypic discrimination of Aeromonas strains isolated from municipally treated tap water samples by cultivation and AP-PCR. Int J Food Microbiol 107:310–314

    Article  CAS  PubMed  Google Scholar 

  • Frederick M, Roger B (1994) Current protocols in molecular biology. Wiley, pp 2.4.1–2.4.3

  • Garcia JA, Larsen JL, Dalssgaard I, Pedersen K (2000) Pulsed-field gel electrophoresis analysis of Aeromonas salmonicida spp salmonicida. FEMS Microbiol Lett 190:163–166

    Article  CAS  PubMed  Google Scholar 

  • Grizzle JM, Kiryu Y (1993) Histopathology of gill, liver, and pancreas, and serum enzyme levels of channel catfish infected with Aeromonas hydrophila complex. J Aquat Anim Health 5:36–50

    Article  Google Scholar 

  • Handfield M, Simard P, Couillard M, Letarte R (1996) Aeromonas hydrophila isolated from food and drinking water: hemagglutination, hemolysis, and cytotoxicity for a human intestinal cell line (HT-29). Appl Environ Microbiol 62:3459–3461

    CAS  PubMed  Google Scholar 

  • Janda JM (1991) Recent advances in the study of the taxonomy, pathogenicity, and infectious syndromes associated with the genus Aeromonas. Clin Microbiol Rev 4:397–410

    CAS  PubMed  Google Scholar 

  • Janda JM, Duffey PS (1988) Mesophilic aeromonads in human disease: current taxonomy, laboratory identification, and infectious disease spectrum. Rev Infect Dis 10:980–997

    CAS  PubMed  Google Scholar 

  • Kanai K, Wakaboyashi H (1984) Purification and some properties of protease from A. hydrophila. Bull Jpn Soc Sci Fish 50:1367–1374

    Google Scholar 

  • Kou GH (1972) Studies on the occurrence and biochemical properties of virulent and avirulent strains of freshwater fish pathogen, Aeromonas liquefaciens. J Fish Soc Taiwan 1:8–13

    Google Scholar 

  • Kühn I, Albert MJ, Ansaruzzaman M, Bhuiyan NA, Alabi SA, Sirajul Islam M, Neogi PKB, Huys G, Janssen P, Kersters K, Mllby R (1997) Characterization of Aeromonas spp. isolated from humans with diarrhea, from healthy controls, and from surface water in Bangladesh. J Clin Microbiol 35:369–373

    PubMed  Google Scholar 

  • Leung KY, Stevenson RMW (1988) Tn5-induced protease-deficient strains of Aeromonas hydrophila with reduced virulence for fish. Infect Immun 56:2639–2644

    CAS  PubMed  Google Scholar 

  • Motyl MR, McKinley G, Janda JM (1985) In vitro susceptibilities of Aeromonas hydrophila, Aeromonas sobria, and Aeromonas caviae to 22 antimicrobial agents. Antimicrob Agents Chemother 28:151–153

    CAS  PubMed  Google Scholar 

  • NCCLS (1997) Performance standards for antimicrobial disks susceptibility tests approved standard M2-A6. National Committee for Clinical Laboratory Standards, Wayne, Pa

  • Osorio CR, Toranzo AE (2002) DNA-based diagnostics in sea farming. In: Fingerman M, Nagabhushanam R (eds) Recent advances in marine biotechnology, seafood and human health, vol 7. Science Publishers Inc., Enfield (NH), USA, pp 253–310

    Google Scholar 

  • Paniagua C, Rivero O, Anguita J, Naharro G (1990) Pathogenicity factors and virulence for rainbow trout (Salmo gairdneri) of motile Aeromonas spp. isolated form a river. J Clin Microbiol 28:350–355

    CAS  PubMed  Google Scholar 

  • Pansare AC, Lewis NF, Venugopal V (1986) Characterization of extracellular protease of Aromonas hydrophila. Agric Biol Chem 50:1743–1749

    CAS  Google Scholar 

  • Pemberton JM, Kidd SP, Schmidt R (1997) Secreted enzymes of Aeromonas. FEMS Microbiol Lett 152:1–10

    Article  CAS  PubMed  Google Scholar 

  • Popoff M (1984) Genus III Aeromonas. In: Krieg NR, Holt JG (eds) Bergy’s manual of determinative bacteriology, vol 1. Williams and Wilkins, Baltimore, MD, USA, pp 545–548

    Google Scholar 

  • Reinhard JF, George WL (1985) Comparative in vitro activities of selected antimicrobial agents against Aeromonas species and Plesiomonas shigelloides. Antimicrob Agents Chemother 27:643–645

    Google Scholar 

  • Roberts RJ (2004) Fish pathology, 3rd edn. W.B. Saunders, Philadeiphia, PA

    Google Scholar 

  • Romalde JL, Toranzo AE (2002) Molecular aproaches for the study and diagnosis of salmonid estreptococcosis. In: Cunningham C (ed) Molecular diagnosis of salmonid diseases. Kluwer Academic Publishers, Dordrecht, The Netherlands, pp 211–233

    Google Scholar 

  • Sadek S, Baraneya A (1993) Evaluation of Egyptian trial in aquaculture. Proceedings of Fish Culture Workshop, Riyadh, Saudi Arabia 27–27

  • Sakai DK (1985) Loss of virulence in a protease-deficient mutant of A. salmonicida. Infect Immun 48:146–152

    CAS  PubMed  Google Scholar 

  • Santos Y, Toranzo AE, Barja JL, Nieto TP, Villa TG (1988) Virulence properties and enterotoxin production of Aeromonas strains isolated from fish. Infect Immun 56:3285–3293

    CAS  PubMed  Google Scholar 

  • Sha J, Kozlova EV, Chopra AK (2002) Role of various enterotoxins in Aeromonas hydrophila—induced gastroenteritis: generation of enterotoxin gene deficient mutants and evaluation of their enterotoxic activity. Infect Immun 70:1924–1935

    Article  CAS  PubMed  Google Scholar 

  • Shotts EB, Rimler RB (1973) Medium for isolation of Aeromonas hydrophila. Appl Microbiol 26:550–553

    PubMed  Google Scholar 

  • Stelma GN, Johnson CH, Spaulding P (1986) Evidence for direct involvement of beta hemolytic in Aeromonas hydrophila enteropathogenicity. Curr Microbiol 14:71–77

    Article  CAS  Google Scholar 

  • Szczuka E, Kaznowski A (2007) Characterization of Aeromonas caviae and A. veronii by standardized cellular protein electrophoretic patterns. Folia Microbiol 52:65–69

    Article  CAS  Google Scholar 

  • Thune RL, Graham TE, Riddle LM, Amborski RL (1982) Extracellular proteases from Aeromonas hydrophila: partial purification and effects on age-0 channel catfish. Trans Am Fish Soc 111:749–754

    Article  CAS  Google Scholar 

  • Ventura MT, Grizzle JM (1988) Lesions associated with natural and experimental infections of Aeromonas hydrophila in channel catfish, Ictalurus punctatus (Rafinesque). J Fish Dis 11:397–407

    Article  Google Scholar 

  • Wang G, Clark CG, Liu C, Pucknell D, Munro CK, Kruk TMAC, Caldeira R, Woodward DL, Rodgers RG (2003) Detection and characterization of the aerolysin genes in Aeromonas hydrophila and Aeromonas sobria by multiplex CR. J Clin Microbiol 41:1048–1054

    Article  CAS  PubMed  Google Scholar 

  • Wong FYK, Fowler K, Desmarchelier PM (1995) Vibriosis due to Vibrio mimicus in Australian freshwater crayfish. J Aquat Anim Health 7:284–291

    Article  Google Scholar 

  • Wu CJ, Wu JJ, Yan JJ, Lee HC, Lee NY, Chang CM, Shih HI, Wu HM, Wang LR, Ko WC (2007) Clinical significance and distribution of putative virulence markers of 116 consecutive clinical Aeromonas isolates in southern Taiwan. J Infect 54:151–158

    Article  PubMed  Google Scholar 

  • Xu D, Rogers WA (1994) OTC residue in striped bass muscle. J Aquat Anim Health 6:349–354

    Article  Google Scholar 

  • Yu HB, Zang YL, Lau YL, Yao F, Vilches S, Merino S, Tomas JM, Howard SP, Leung KY (2005) Identification and characterization of putative virulence genes and gene clusters in Aeromonas hydrophila PPD134/91. Appl Environ Microbiol 71:4469–4477

    Article  CAS  PubMed  Google Scholar 

  • Zhang YL, Ong CT, Leung KY (2000) Molecular analysis of genetic differences between virulent and avirulent strains of Aeromonas hydrophila isolated from diseased fish. Microbiology 146:999–1009

    CAS  PubMed  Google Scholar 

Download references

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  1. Aquatic Pathology Laboratory, National Institute of Oceanography and Fisheries, Cairo, Egypt

    Manal I. El-Barbary

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  1. Manal I. El-Barbary
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Correspondence to Manal I. El-Barbary.

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El-Barbary, M.I. Some clinical, microbiological and molecular characteristics of Aeromonas hydrophila isolated from various naturally infected fishes. Aquacult Int 18, 943–954 (2010). https://doi.org/10.1007/s10499-009-9315-x

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