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Evidence for the direct involvement ofβ-hemolysin inAeromonas hydrophila enteropathogenicity

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Abstract

Culture filtrates of 19 of 21 (90%) β-hemolytic isolates ofAeromonas hydrophila caused fluid accumulation in permanently ligated rabbit ileal loops, whereas no fluid was accumulated with filtrates of eight non-β-hemolytic isolates. Antiserum to purified β-hemolysin neutralized the ileal loop activity of culture filtrates from four of four β-hemolytic isolates, and treatment at 56°C for 10 min eliminated the loop activity of six additional isolates. These results support the conclusion that β-hemolysin alone causes significant changes in intestinal permeability and that it is a more common pathogenic mechanism than the heat-stable cytotonic enterotoxin. Electrophoretic and serological assays showed evidence for production of only one species of β-hemolysin byA. hydrophila.

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Authors and Affiliations

  1. Division of Microbiology, Food and Drug Administration, 45226, Cincinnati, Ohio, USA

    Gerard N. Stelma Jr., Clifford H. Johnson & Procter Spaulding

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  1. Gerard N. Stelma Jr.
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  2. Clifford H. Johnson
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  3. Procter Spaulding
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Stelma, G.N., Johnson, C.H. & Spaulding, P. Evidence for the direct involvement ofβ-hemolysin inAeromonas hydrophila enteropathogenicity. Current Microbiology 14, 71–77 (1986). https://doi.org/10.1007/BF01568406

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