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Labilibaculum antarcticum sp. nov., a novel facultative anaerobic, psychrotorelant bacterium isolated from marine sediment of Antarctica

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Abstract

A novel facultative anaerobic and facultative psychrophilic bacterium, designated SPP2T, was isolated from an Antarctic marine sediment. Cells of the isolate were observed to be long rods (0.5 × 5–10 μm), Gram-stain negative and to have gliding motility. For growth, the optimum NaCl concentration was found to be 2–3% and the optimum temperature to be 18–22 °C. Strain SPP2T cannot use sulfate and nitrate as electron acceptors in the presence of lactate. The G+C content of the genomic DNA was determined to be 36.0 mol%.. The major cellular fatty acids were identified as anteiso-C15:0 and iso-C15:0. MK-7 was found to be the predominant respiratory quinone. Phylogenetic analysis based on the 16S rRNA gene revealed that the novel strain belongs to the family Marinifilaceae and to be closely related to Labilibaculum manganireducens 59.10-2MT with 16S rRNA gene sequence identity of 98%. The OrthoANI and dDDH values between the genome sequences of strain SPP2T and its close relative were 84% and 27.3%, which are lower than the threshold values for species delineation. On the basis of phylogenetic and phenotypic characterisation, Labilibaculum antarcticum sp. nov. is proposed with the type strain SPP2T (= NBRC 111151T = CECT 9460T).

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Acknowledgements

The authors thank R. Tokizawa and A. Shinohara, Hokkaido University, for their technical assistance.

Funding

This study was supported by JSPS KAKENHI Grant Number 22370005 to Fukui and Research Fellow of Japan Society for the Promotion Science to Watanabe.

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Author notes

  1. Miho Watanabe

    Present address: Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Ten-nodai, Tsukuba, Ibaraki, 305-8572, Japan

Authors and Affiliations

  1. The Institute of Low Temperature Science, Hokkaido University, Kita-19, Nishi-8, Kita-ku, Sapporo, 060-0819, Japan

    Miho Watanabe, Hisaya Kojima & Manabu Fukui

  2. Japan Society for the Promotion of Science, Chiyoda-ku, Tokyo, 102-8471, Japan

    Miho Watanabe

Authors
  1. Miho Watanabe
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  2. Hisaya Kojima
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  3. Manabu Fukui
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Contributions

MF collected the sediment sample and supervised the study. MW and HK conducted experiments. MW performed computational analyses. MW and HK wrote the manuscript. All authors discussed the data and approved the final manuscript.

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Correspondence to Miho Watanabe.

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Watanabe, M., Kojima, H. & Fukui, M. Labilibaculum antarcticum sp. nov., a novel facultative anaerobic, psychrotorelant bacterium isolated from marine sediment of Antarctica. Antonie van Leeuwenhoek 113, 349–355 (2020). https://doi.org/10.1007/s10482-019-01345-w

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