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Pusillimonas caeni sp. nov., isolated from a sludge sample of a biofilm reactor

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Abstract

A polyphasic taxonomic study was carried out on strain EBR-8-1T isolated from a biofilm reactor in Korea. The cells of the strain were Gram-stain negative, non-spore-forming, non-motile, and short rod-shaped. Comparative 16S rRNA gene sequence studies showed a clear affiliation of this strain with Betaproteobacteria, which showed high pairwise sequence similarities with Pusillimonas noertemannii BN9T (99.1 %), Pusillimonas soli MJ07T (97.3 %), Pusillimonas ginsengisoli DCY25T (97.2 %), and Pusillimonas harenae B201T (96.8 %). The phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a clear phylogenetic lineage within the genus Pusillimonas. The major fatty acids were identified as C16:0, C17:0 cyclo and C19:0 cyclo ω8c. The major cellular polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and an unidentified aminolipid. The respiratory quinone was identified as Q-8 and the genomic DNA G+C content was determined to be 63.3 mol%. On the basis of polyphasic evidence, it is proposed that strain EBR-8-1T should be placed in a new species, Pusillimonas caeni sp. nov. The type stain is EBR-8-1T (=KCTC 42353T = JCM 30463T).

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Acknowledgments

This research was supported by Grants from the Advanced Biomass R&D Centre, the Global Frontier Program funded by the Korean Ministry of Science, ICT and Future Planning, the KRIBB Research Initiative Program, and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. College of Biology and the Environment, Co-Innovation Centre for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, 210-037, China

    Long Jin

  2. Cell Factory Research Centre, Korea Research Institute of Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, Republic of Korea

    So-Ra Ko, Yingshun Cui, Hee-Mock Oh, Chi-Yong Ahn & Hyung-Gwan Lee

  3. Culture Collection Team, Freshwater Bioresources Culture Research Division, Nakdonggang National Institute of Biological Resources, 137 Donam 2, Sangju City, Gyeongsang Province, 37242, Republic of Korea

    Chang Soo Lee

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  1. Long Jin
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  3. Yingshun Cui
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  4. Chang Soo Lee
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Correspondence to Chi-Yong Ahn or Hyung-Gwan Lee.

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Jin, L., Ko, SR., Cui, Y. et al. Pusillimonas caeni sp. nov., isolated from a sludge sample of a biofilm reactor. Antonie van Leeuwenhoek 110, 125–132 (2017). https://doi.org/10.1007/s10482-016-0782-6

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  • DOI: https://doi.org/10.1007/s10482-016-0782-6

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