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Paenibacillus oryzisoli sp. nov., isolated from the rhizosphere of rice

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Abstract

A novel bacterium, strain 1ZS3-15T, was isolated from rhizosphere of rice. Its taxonomic position was investigated using a polyphasic approach. The novel strain was observed to be Gram-stain positive, spore-forming, aerobic, motile and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 1ZS3-15T was recovered within the genus Paenibacillus. It is closely related to Paenibacillus pectinilyticus KCTC 13222T (97.9 % similarity), Paenibacillus frigoriresistens CCTCC AB 2011150T (96.8 %), Paenibacillus alginolyticus JCM 9068T (96.4 %) and Paenibacillus chondroitinus DSM 5051T (95.5 %). The fatty acid profile of strain 1ZS3-15T, which showed a predominance of anteiso-C15:0 and iso-C16:0, supported the allocation of the strain into the genus Paenibacillus. The predominant menaquinone was found to be MK-7. The polar lipids profile of strain 1ZS3-15T was found to consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified lipid and two unidentified aminophospholipids. The cell wall peptidoglycan contains meso-diaminopimelic acid. Based on draft genome sequences, the DNA–DNA relatedness between strain 1ZS3-15T and the closely related species P. pectinilyticus KCTC 13222T are 24.2 ± 1.0 %, and the Average Nucleotide Identity values between the strains are 78.9 ± 0.1 %, which demonstrated that this isolate represents a new species in the genus Paenibacillus. The DNA G+C content was determined to be 45.3 mol%, which is within the range reported for Paenibacillus species. Characterisation by genotypic, chemotaxonomic and phenotypic analysis indicated that strain 1ZS3-15T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus oryzisoli sp. nov. is proposed. The type strain is 1ZS3-15T (= ACCC 19783T = JCM 30487T).

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Acknowledgments

This work was supported by the Grants from National Natural Science Foundation of China (41271273), the Chinese Ministry of Science and Technology (2013AA102802) and the Key ‘‘948’’ Project of Ministry of Agriculture of China (2011-G25).

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Authors and Affiliations

  1. College of Life Science, The Yangtze University, Jingzhou, 434025, People’s Republic of China

    Jun Zhang & Li-An Ma

  2. Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People’s Republic of China

    Jun Zhang, Xiao-Tong Ma, Cai-Wen Zhang, Juan-Juan Zhao, Rui-Jie Zhang & Xiao-Xia Zhang

  3. Qiyang Agro-ecosystem of National Field Experimental Station, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Qiyang, 426182, People’s Republic of China

    Jun-Sheng Gao

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  1. Jun Zhang
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Correspondence to Li-An Ma or Xiao-Xia Zhang.

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Zhang, J., Ma, XT., Gao, JS. et al. Paenibacillus oryzisoli sp. nov., isolated from the rhizosphere of rice. Antonie van Leeuwenhoek 110, 69–75 (2017). https://doi.org/10.1007/s10482-016-0777-3

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  • DOI: https://doi.org/10.1007/s10482-016-0777-3

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