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Aeromonas and Plesiomonas species from scarlet ibis (Eudocimus ruber) and their environment: monitoring antimicrobial susceptibility and virulence

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Abstract

The present study aimed at evaluating the role of captive scarlet ibises (Eudocimus ruber) and their environment as reservoirs of Aeromonas spp. and Plesiomonas spp., and analyzing the in vitro antimicrobial susceptibility and virulence of the recovered bacterial isolates. Thus, non-lactose and weak-lactose fermenting, oxidase positive Gram-negative bacilli were recovered from cloacal samples (n = 30) of scarlet ibises kept in a conservational facility and from water samples (n = 30) from their environment. Then, the antimicrobial susceptibility, hemolytic activity and biofilm production of the recovered Aeromonas spp. and Plesiomonas shigelloides strains were assessed. In addition, the virulence-associated genes of Aeromonas spp. were detected. Ten Aeromonas veronii bv. sobria, 2 Aeromonas hydrophila complex and 10 P. shigelloides were recovered. Intermediate susceptibility to piperacillin-tazobactam and cefepime was observed in 2 Aeromonas spp. and 1 P. shigelloides, respectively, and resistance to gentamicin was observed in 4 P. shigelloides. The automated susceptibility analysis revealed resistance to piperacillin-tazobactam and meropenem among Aeromonas spp. and intermediate susceptibility to gentamicin among P. shigelloides. All Aeromonas isolates presented hemolytic activity, while 3 P. shigelloides were non-hemolytic. All Aeromonas spp. and 3/10 P. shigelloides were biofilm-producers, at 28 °C, while 10 Aeromonas spp. and 6/10 P. shigelloides produced biofilms, at 37 °C. The most prevalent virulence genes of Aeromonas spp. were asa1 and ascV. Scarlet ibises and their environment harbour potentially pathogenic bacteria, thus requiring monitoring and measures to prevent contamination of humans and other animals.

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Acknowledgements

This work was supported by the National Council for Scientific and Technological Development (CNPq; Brazil; process: 445670/2014-2) and Coordination Office for the Improvement of Higher Education Personnel (CAPES; Brazil, Procad/Casadinho; process: 552215/2011-2). We thank Parque Mangal das Garças for allowing the performance of this research in their facility.

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Authors and Affiliations

  1. Department of Pathology and Legal Medicine, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Rua Coronel Nunes de Melo s/n, Rodolfo Teófilo, Fortaleza, Ceará, CEP 60430-270, Brazil

    Débora Souza Collares Maia Castelo-Branco, Glaucia Morgana de Melo Guedes, Jonathas Sales de Oliveira, José Júlio Costa Sidrim, Lucas Pereira de Alencar, Raimunda Sâmia Nogueira Brilhante, Rossana de Aguiar Cordeiro, Tereza de Jesus Pinheiro Gomes Bandeira, Waldemiro de Aquino Pereira Neto & Marcos Fábio Gadelha Rocha

  2. School of Veterinary Medicine, Postgraduate Program in Veterinary Sciences, State University of Ceará, Fortaleza, Ceará, Brazil

    Aline Lobão da Silva, Jamille Alencar Sales, José Erisvaldo Maia Junior & Marcos Fábio Gadelha Rocha

  3. Universidade Federal Rural da Amazônia – UFRA, Belém, Pará, Brazil

    Frederico Ozanan Barros Monteiro

  4. Parque Mangal das Garças, Belém, Pará, Brazil

    Stefânia Araújo Miranda

  5. School of Medicine, Christus College – Unichristus, Fortaleza, Ceará, Brazil

    Tereza de Jesus Pinheiro Gomes Bandeira

Authors
  1. Débora Souza Collares Maia Castelo-Branco
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  2. Aline Lobão da Silva
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  3. Frederico Ozanan Barros Monteiro
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  4. Glaucia Morgana de Melo Guedes
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  6. Jonathas Sales de Oliveira
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  8. Stefânia Araújo Miranda
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  9. José Júlio Costa Sidrim
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  11. Raimunda Sâmia Nogueira Brilhante
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  12. Rossana de Aguiar Cordeiro
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  13. Tereza de Jesus Pinheiro Gomes Bandeira
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  14. Waldemiro de Aquino Pereira Neto
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  15. Marcos Fábio Gadelha Rocha
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Corresponding author

Correspondence to Raimunda Sâmia Nogueira Brilhante.

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Castelo-Branco, D.S.C.M., Silva, A.L., Monteiro, F.O.B. et al. Aeromonas and Plesiomonas species from scarlet ibis (Eudocimus ruber) and their environment: monitoring antimicrobial susceptibility and virulence. Antonie van Leeuwenhoek 110, 33–43 (2017). https://doi.org/10.1007/s10482-016-0771-9

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