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Abyssicoccus albus gen. nov., sp. nov., a novel member of the family Staphylococcaceae isolated from marine sediment of the Indian Ocean

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An Erratum to this article was published on 08 August 2016

Abstract

A Gram-stain positive, aerobic, non-motile, asporogenous, coccoid shaped bacterium, designated YIM M12140T, was isolated from a marine sediment sample collected from the Indian Ocean. Phylogenetic analysis showed that strain YIM M12140T forms a separate clade within the family Staphylococcaceae. Strain YIM M12140T shares high 16S rRNA gene sequence similarity with Macrococcus brunensis DSM 19358T (92.9 %). The isolate was found to grow at 0–10 % (w/v) NaCl (optimum, 2–3 %), pH 6.0–10.0 (optimum, pH 8.0) and temperature 5–40 °C (optimum, 28 °C). The polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, one unidentified aminophospholipid and two unidentified polar lipids. The major cellular fatty acids of the strain were identified as anteiso-C15:0, -C17:0, iso-C16:0, anteiso-C19:0 and C20:0. The respiratory menaquinones were found to be MK-6 (94 %) and MK-7 (6 %). The cell wall amino acids were found to contain Lys, Ala, Glu, Gly, Asp, Ser and Thr. Whole cell sugars were identified as mannose, ribose, rhamnose, glucose, galactose and xylose. The G+C content of the genomic DNA of strain YIM M12140T was determined to be 42.4 mol %. Based on phenotypic, chemotaxonomic data and phylogenetic analysis, it is proposed that strain YIM M12140T represents a novel species of a new genus in the family Staphylococcaceae, for which the name Abyssicoccus albus gen. nov., sp. nov. is proposed. The type strain is YIM M12140T (= DSM 29158T = CCTCC AB 2014213T).

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Acknowledgments

The authors are grateful to Dr. Rüdiger Pukall (DSMZ, Germany) for providing the reference type strain and Dr. Nimaichand Salam (Manipur University, India) for his help further polishing this manuscript. This research was supported by the National Basic Research Program of China (No. 2010CB833801), Ministry of Science, ICT and Future Planning of Korean government (NRF-2013M3A9A5076601). W-J Li was also supported by Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2014).

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    Authors and Affiliations

    1. Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

      Zhao Jiang, Chang-Guo Yuan, Inam-Ullah Khan, Xiao-Yang Zhi & Wen-Jun Li

    2. State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

      Min Xiao & Wen-Jun Li

    3. Key Laboratory of Tropical Marine Bio-resources and Ecology, CAS; RNAM Center for Marine Microbiology, CAS; Guangdong Key Laboratory of Marine Materia Medica; South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, People’s Republic of China

      Xin-Peng Tian

    4. Microbial Resource Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon, 305-806, Republic of Korea

      Chang-Jin Kim

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    Correspondence to Xiao-Yang Zhi or Wen-Jun Li.

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    Zhao Jiang and Chang-Guo Yuan have contributed equally to this work.

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    Jiang, Z., Yuan, CG., Xiao, M. et al. Abyssicoccus albus gen. nov., sp. nov., a novel member of the family Staphylococcaceae isolated from marine sediment of the Indian Ocean. Antonie van Leeuwenhoek 109, 1153–1160 (2016). https://doi.org/10.1007/s10482-016-0717-2

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