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Halomonas heilongjiangensis sp. nov., a novel moderately halophilic bacterium isolated from saline and alkaline soil

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Abstract

A moderately halophilic bacterium, designated strain 9-2T, was isolated from saline and alkaline soil collected in Lindian county, Heilongjiang province, China. The strain was observed to be strictly aerobic, Gram-negative, rod-shaped, oxidase-positive, catalase-positive and motile. It was found to require NaCl for growth and to grow at NaCl concentrations of 0.5–14 % (w/v) (optimum, 7–10 %, w/v), at temperatures of 10–45 °C (optimum 25–30 °C) and at pH 5.0–10.0 (optimum pH 8.0). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 9-2T is a member of the genus Halomonas and is closely related to Halomonas desiderata DSM 9502T (96.68 %), Halomonas campaniensis DSM 1293T (96.46 %), Halomonas ventosae DSM 15911T (96.27 %) and Halomonas kenyensis DSM 17331T (96.27 %). The DNA–DNA hybridization value was 38.9 ± 0.66 % between the novel isolate 9-2T and H. desiderata DSM 9502T. The predominant ubiquinones were identified as Q9 (75.1 %) and Q8 (24.9 %). The major fatty acids were identified as C16:0 (22.0 %), Summed feature 8 (C18:1 ω6c/C18:1 ω7c, 19.6 %), Summed feature 3 (C16:1 ω6c/C16:1 ω7c, 12.6 %), C12:0 3-OH (12.0 %) and C10:0 (11.7 %). The DNA G+C content was determined to be 69.7 mol%. On the basis of the evidence presented in this study, strain 9-2T is considered to represent a novel species of the genus Halomonas, for which the name Halomonas heilongjiangensis sp. nov. is proposed. The type strain is 9-2T (=DSM 26881T = CGMCC 1.12467T).

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Acknowledgments

We thank Yuqin Zhang of the Institute of Medicinal Biotechnology, Academy of Medical Sciences for the respiratory menaquinone analysis. This work was supported by the Forestry Industry Research Special Funds for Public Welfare Projects (201304409), the Beijing Nova Program (2011033), the Fundamental Research Funds for the Central Universities (YX2014-15), National Science and Technology Ministry (2012BAC09B03) and the National Science Foundation of China (J1103516).

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Authors and Affiliations

  1. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, People’s Republic of China

    Guiming Dou & Yuchao Ma

  2. College of Forestry, Beijing Forestry University, Beijing, 100083, People’s Republic of China

    Wei He

  3. Institute of Microbiology, China General Microbiological Culture Collection Center, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China

    Hongcan Liu

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  1. Guiming Dou
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Dou, G., He, W., Liu, H. et al. Halomonas heilongjiangensis sp. nov., a novel moderately halophilic bacterium isolated from saline and alkaline soil. Antonie van Leeuwenhoek 108, 403–413 (2015). https://doi.org/10.1007/s10482-015-0493-4

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