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Isolation and classification of a novel marine Bacteroidetes as Frondibacter aureus gen. nov., sp. nov.

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Abstract

A facultatively anaerobic, Gram-stain negative, golden-yellow pigmented, non-motile and rod-shaped bacterium, designated strain A5Q-67T was isolated from leaf litter collected at the mangrove estuary of Nakama River, Japan. Phylogenetic analysis based on the 16S rRNA gene sequence revealed the novel isolate was affiliated with the family Flavobacteriaceae of the phylum Bacteroidetes and that it showed highest sequence similarity (94.2 %) to Imtechella halotolerans K1T. The strain could be differentiated phenotypically from recognized members of the family Flavobacteriaceae. The major fatty acids of strain A5Q-67T were identified as iso-C17:0 3-OH, summed feature 1 (iso-C15:1 H and/or C13:0 3-OH) and iso-C15:0 as defined by the MIDI system. The DNA G+C content was determined to be 36.7 mol%, the major respiratory quinone was identified as menaquinone 6 (MK-6) and a polar lipid profile was present consisting of phosphatidylethanolamine, two unidentified aminolipids and an unidentified lipid. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain is considered to represent a novel genus for which the name Frondibacter aureus gen. nov., sp. nov. is proposed. The type strain of F. aureus is A5Q-67T (=KCTC 32991T = NBRC 110021T).

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Acknowledgments

This study was supported by Dongil Culture & Scholarship Foundation for the grant in 2014.

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Author notes

  1. Kyoko Adachi

    Present address: Basic Research Laboratories, Nippon Steel & Sumikin Chemical Co., Ltd., 1-Tsukiji, Kisarazu, Chiba, 292-0835, Japan

Authors and Affiliations

  1. College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 704-701, Republic of Korea

    Jaewoo Yoon

  2. Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate, 026-0001, Japan

    Kyoko Adachi

  3. Marine Biosciences Kamaishi Research Laboratory, Kitasato University, 160-4 Utou, Okirai, Sanriku-cho, Ofunato, Iwate, 022-0101, Japan

    Hiroaki Kasai

Authors
  1. Jaewoo Yoon
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Correspondence to Jaewoo Yoon.

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10482_2014_330_MOESM1_ESM.ppt

Supplementary material 1 (PPT 666 kb). Supplementary Fig. 1 Thin-layer chromatograms showing the total polar lipid compositions of A5Q-67T. Total polar lipids were detected by spraying the plate with molybdophosphoric acid, molybdenun blue, α-naphthol and ninhydrin. PE phosphatidylenthanolamine, UAL unidentified aminolipid, UL unidentified lipid

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Yoon, J., Adachi, K. & Kasai, H. Isolation and classification of a novel marine Bacteroidetes as Frondibacter aureus gen. nov., sp. nov.. Antonie van Leeuwenhoek 107, 321–328 (2015). https://doi.org/10.1007/s10482-014-0330-1

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  • DOI: https://doi.org/10.1007/s10482-014-0330-1

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