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Cohnella capsici sp. nov., a novel nitrogen-fixing species isolated from Capsicum annuum rhizosphere soil, and emended description of Cohnella plantaginis

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Abstract

A novel bacterial strain designated YN-59T was isolated from Capsicum annuum rhizosphere soil in China. The isolate was found to be aerobic, Gram-positive, rod-shaped and to form ellipsoidal or oval spores positioned centrally in swollen sporangia. On the basis of 16S rRNA gene sequence analysis, the isolated strain YN-59 was determined to be related to members of genus Cohnella. High levels of 16S rRNA gene sequence similarity were found between strain YN-59 and Cohnella plantaginis DSM 25424T (98.5 %) and Cohnella ginsengisoli DSM18997T (97.3 %); the 16S rRNA gene sequence similarities between strain YN-59 and the other strains recognized members of the genus Cohnella were below 97 %. The DNA–DNA hybridization values of strain YN-59 with C. plantaginis DSM 25424T and C. ginsengisoli DSM18997T were 44.2 ± 8.4 and 28.8 ± 5.8 %, respectively. The DNA G + C content of strain YN-59T was determined to be 59.32 mol %. The major isoprenoid quinone was identified as MK-7 and the predominant fatty acids as anteiso-C15:0 (45.32 %), iso-C16:0 (19.19 %), iso-C15:0 (9.65 %) and C16:0 (8.91 %). The polar lipids of strain YN-59T were found to consist of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol; several unidentified phospholipids were also detected. The diagnostic diamino acid in the cell wall was identified as meso-diaminopimelic. On the basis of its phenotypic and genotypic characteristics and levels of DNA–DNA hybridization, strain YN-59T is considered to represent a novel species of the genus Cohnella, for which the name Cohnella capsici sp. nov. (type strain YN-59T = CGMCC 1.12046T = JCM 19168T) is proposed.

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Acknowledgments

We thank Dr Soon-Wo Kwon for providingthe reference type strain. We are grateful to Ph.D.Takuji Kudo for depositing the strain in JCM. This work was supported by the 863 High Technology Program (Grant No. 2013AA102802-04).

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Authors and Affiliations

  1. State Key Laboratory for Agrobiotechnology, Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China

    Li-Ying Wang, Tian-Shu Wang & San-Feng Chen

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  1. Li-Ying Wang
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  2. Tian-Shu Wang
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  3. San-Feng Chen
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Correspondence to San-Feng Chen.

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Wang, LY., Wang, TS. & Chen, SF. Cohnella capsici sp. nov., a novel nitrogen-fixing species isolated from Capsicum annuum rhizosphere soil, and emended description of Cohnella plantaginis . Antonie van Leeuwenhoek 107, 133–139 (2015). https://doi.org/10.1007/s10482-014-0310-5

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