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Staurosporine from the endophytic Streptomyces sp. strain CNS-42 acts as a potential biocontrol agent and growth elicitor in cucumber

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Abstract

Chinese medicinal plants and their surrounding rhizospheric soil serve as promising sources of actinobacteria. A total of 180 actinobacteria strains were isolated from the rhizosphere soil, leaves, stems, and roots of nine selected plants and have been identified as potential biocontrol agents against Fusarium oxysporum f. sp. cucumerinum. An endophytic strain CNS-42 isolated from Alisma orientale showed the largest zone of inhibition demonstrating a potent effect against F. oxysporum f. sp. cucumerinum and a broad antimicrobial activity against bacteria, yeasts, and other pathogenic fungi. The in vivo biocontrol assays showed that the disease severity index was significantly reduced (P < 0.05), and plant shoot fresh weight and height increased greatly (P < 0.05) in plantlets treated with strain CNS-42 compared to the negative control. This isolate was identified as Streptomyces sp. based on cultural, physiological, morphological characteristics, and 16S rRNA gene analysis. Further bioassay-guided isolation and purification revealed that staurosporine was responsible for its antifungal and plant growth promoting activities and the latter property of staurosporine is reported for the first time. The in vivo assay was further performed and indicated that staurosporine showed good growth promoting effect on the plant shoot biomass of cucumber. This is the first critical evidence identifying CNS-42 as a biocontrol agent for the soil borne pathogen, F. oxysporum f. sp. cucumerinum.

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Acknowledgments

The authors are grateful to Dr. Qi Li of Chinese Academy of Sciences and Prof. Isoamro Yamaguchi of The University of Tokyo, for kindly correcting this manuscript. This work was supported in parts by the National Program on Key Basic Research Project (2013AA102802, 2013ZX10005004 and 2011ZX11102-011-11), the Chinese National Natural Science Fund (31070004, 81102369, 30911120483, 81102356, 30901849, 30973665, 30911120484) and the specialized research fund for the doctoral program of higher education 20060626006. LZ is an Awardee for National Distinguished Young Scholar Program in China. Krishna Bolla is thankful to CAS and TWAS for the financial support.

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Authors and Affiliations

  1. Department of Microbiology, College of Resource and Environment, Sichuan Agricultural University, Chengdu, 611130, China

    Xiaolin Li, Lie Xiao, Bo Zhang & Xiaoping Zhang

  2. Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China

    Pei Huang, Qian Wang, Miaomiao Liu, Krishna Bolla, Xueting Liu & Lixin Zhang

  3. Soil and Fertilizer Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China

    Xiaolin Li, Linyong Zheng & Bingcheng Gan

  4. University of Chinese Academy of Sciences, Beijing, 100049, China

    Pei Huang, Qian Wang & Miaomiao Liu

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  1. Xiaolin Li
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Correspondence to Xueting Liu, Lixin Zhang or Xiaoping Zhang.

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Xiaolin Li and Pei Huang have contributed equally to this work.

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Li, X., Huang, P., Wang, Q. et al. Staurosporine from the endophytic Streptomyces sp. strain CNS-42 acts as a potential biocontrol agent and growth elicitor in cucumber. Antonie van Leeuwenhoek 106, 515–525 (2014). https://doi.org/10.1007/s10482-014-0220-6

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