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Verrucosispora maris sp. nov., a novel deep-sea actinomycete isolated from a marine sediment which produces abyssomicins

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Abstract

Verrucosispora isolate AB-18-032T, the abyssomicin- and proximicin-producing actinomycete, has chemotaxonomic and morphological properties consistent with its classification in the genus Verrucosispora. The organism formed a distinct phyletic line in the Verrucosispora 16S rRNA gene tree sharing similarities of 99.7%, 98.7% and 98.9% with Verrucosispora gifhornensis DSM 44337T, Verrucosispora lutea YIM 013T and Verrucosispora sediminis MS 426T, respectively. It was readily distinguished from the two latter species using a range of phenotypic features and from V. gifhornensis DSM 44337T, its nearest phylogenetic neighbor, by a DNA G+C content of 65.5 mol% obtained by thermal denaturation and fluorometry and DNA:DNA relatedness values of 64.0% and 65.0% using renaturation and fluorometric methods, respectively. It is apparent from the combined genotypic and phenotypic data that strain AB-18-032T should be classified in the genus Verrucosispora as a new species. The name Verrucosispora maris sp. nov. is proposed for this taxon with isolate AB-18-032T (= DSM 45365T = NRRL B-24793T) as the type strain.

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Acknowledgments

ATB and MG were supported by the UK Natural Environmental Research Council (grants NER/T/S/2000/00614 and NER/T/S/2000/00616), JEMS by the Biological and Biotechnological Research Council (BBSRC grant BB/EO17053/1) and TDZ by a Conselho Nacional de DesenvolvImento Cientifico e Tecnológico Fellowship to study in the UK (grant 201066/2009-2). ATB thanks The Leverhulme Trust for an Emeritus Fellowship. The authors are indebted to Professor Koki Horikoshi (JAMSTEC) for the sediment sample.

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Authors and Affiliations

  1. School of Biology, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK

    Michael Goodfellow, James E. M. Stach, Roselyn Brown, Avinash Naga Venkata Bonda, Amanda L. Jones, Tiago Domingues Zucchi & Alan T. Bull

  2. School of Life Sciences, Ellison Building, Northumbria University, Newcastle upon Tyne, NE1 8ST, UK

    Amanda L. Jones

  3. School of Biosciences, University of Kent, Canterbury, CT2 7NJ, UK

    Joanne Mexson & Alan T. Bull

  4. Mikrobiologisches Institut, Universität Tübingen, Auf der Morgenstelle 28, 72076, Tuebingen, Germany

    Hans-Peter Fiedler

  5. Departamento de Entomologia e Acarologia, ESALQ, Universidade de São Paulo, Piracicaba, Brazil

    Tiago Domingues Zucchi

Authors
  1. Michael Goodfellow
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  2. James E. M. Stach
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  3. Roselyn Brown
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  4. Avinash Naga Venkata Bonda
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  7. Hans-Peter Fiedler
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  8. Tiago Domingues Zucchi
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  9. Alan T. Bull
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Corresponding author

Correspondence to Michael Goodfellow.

Additional information

The Genbank accession number for the 16S rRNA gene sequence of Verrucosispora maris AB-18-032T is AY528866.

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Goodfellow, M., Stach, J.E.M., Brown, R. et al. Verrucosispora maris sp. nov., a novel deep-sea actinomycete isolated from a marine sediment which produces abyssomicins. Antonie van Leeuwenhoek 101, 185–193 (2012). https://doi.org/10.1007/s10482-011-9651-5

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  • DOI: https://doi.org/10.1007/s10482-011-9651-5

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