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Candida middelhoveniana sp. nov., a new yeast species found on the rhizoplane of organically cultivated sugarcane

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Abstract

A novel yeast species within the Metschnikowiaceae is described based on a strain from the sugarcane (Saccharum sp.) rhizoplane of an organically managed farm in Rio de Janeiro, Brazil. The D1/D2 domain of the large subunit ribosomal RNA gene sequence analysis showed that the closest related species were Candida tsuchiyae with 86.2% and Candida thailandica with 86.7% of sequence identity. All three are anamorphs in the Clavispora opuntiae clade. The name Candida middelhoveniana sp. nov. is proposed to accommodate this highly divergent organism with the type strain Instituto de Microbiologia, Universidade Federal do Rio de Janeiro (IMUFRJ) 51965T (=Centraalbureau voor Schimmelcultures (CBS) 12306T, Universidade Federal de Minas Gerais (UFMG)-70T, DBVPG 8031T) and the GenBank/EMBL/DDBJ accession number for the D1/D2 domain LSU rDNA sequence is FN428871. The Mycobank deposit number is MB 519801.

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Acknowledgments

We thank Embrapa Agrobiologia, Pesagro/RIO and UFRRJ for permission to collect at the SIPA. Access to EMBL and SILVA/ARB sequence databases and the CBS culture collection database are gratefully acknowledged. This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and FAPERJ/PRONEX. We also acknowledge the use of the DNA sequencing facility at Embrapa Agrobiologia and assistance of Aline Macedo and Dr. Jean Luis Simões Araújo.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Agronomia Ciência do Solo, Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, 23890-000, Brazil

    José R. de A. Ribeiro & Ricardo L. L. Berbara

  2. Programa de Pós-Graduação em Biotecnologia Vegetal, CCS, Bloco K, UFRJ, Ilha do Fundão, Rio de Janeiro, RJ, 21941-590, Brazil

    Patrícia M. B. de Carvalho, Anderson de S. Cabral, Andrew Macrae, Leda C. S. Mendonça-Hagler & Allen N. Hagler

  3. Instituto de Microbiologia Prof. Paulo de Goés, CCS, Bloco I, UFRJ, Ilha do Fundão, Rio de Janeiro, RJ, 21941-590, Brazil

    José R. de A. Ribeiro, Patrícia M. B. de Carvalho, Anderson de S. Cabral, Andrew Macrae, Leda C. S. Mendonça-Hagler & Allen N. Hagler

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  1. José R. de A. Ribeiro
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  2. Patrícia M. B. de Carvalho
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  3. Anderson de S. Cabral
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  6. Ricardo L. L. Berbara
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  7. Allen N. Hagler
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Correspondence to Allen N. Hagler.

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de A. Ribeiro, J.R., de Carvalho, P.M.B., de S. Cabral, A. et al. Candida middelhoveniana sp. nov., a new yeast species found on the rhizoplane of organically cultivated sugarcane. Antonie van Leeuwenhoek 100, 341–347 (2011). https://doi.org/10.1007/s10482-011-9589-7

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  • DOI: https://doi.org/10.1007/s10482-011-9589-7

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