Abstract
Vibrations in research facilities can cause complex animal behavioral and physiological responses that can affect animal health and research outcomes. The goal of this study was to determine the range of frequency values, where animals are unable to attenuate vibrations, and therefore may be most susceptible to their effects. Anesthetized and euthanized adult rats and mice were exposed to vibration frequencies over a wide range (0–600 Hz) and at a constant magnitude of 0.3 m/s2. Euthanized animals were additionally exposed to vibrations at an acceleration of 1 m/s2. The data showed that at most frequencies rodents were able to attenuate vibration magnitudes, with values for the back-mounted accelerometer being substantially less than that of the table. At frequencies of 41–60 Hz mice did not attenuate vibration magnitude, but instead the magnitude of the table and animal were equal or amplified. Rats experienced the same pattern of non-attenuation between 31 and 50 Hz. Once euthanized, the mice vibrated at a slightly more elevated frequency (up to 100 Hz). Based on these results, it may be prudent that in laboratory settings, vibrations in the ranges reported here should be accounted for as possible contributors to animal stress and/or biomechanical changes.
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Abeyesinghe, S. M., C. M. Wathes, C. J. Nicol, and J. M. Randall. The aversion of broiler chickens to concurrent vibrational and thermal stressors. Appl. Anim. Behav. Sci. 73:199–215, 2011.
Ariizumi, M., and A. Okada. Effect of whole-body vibration on the rat brain content of serotonin and plasma corticosterone. Eur. J. Appl. Physiol. 52:15–19, 1983.
Buckley, J. P., and H. H. Smooker. Cardiovascular and biochemical effects of chronic intermittent neurogenic stimulation. In: Physiological Effects of Noise, edited by B. L. Welch, and A. S. Welch. New York: Plenum Press, 1970, pp. 74–85.
Ceccarelli, G., L. Benedetti, D. Galli, D. Prè, G. Silvani, N. Crosetto, G. Magenes, and M. G. C. De Angelis. MGC. Low-amplitude high-frequency vibration down-regulates myostatin and atrogin-1 expression, two components of the atrophy pathway in muscle cells. J. Tissue Eng. Regen. Med. 8:396–406, 2012.
Christiansen, B. A., and M. J. Silva. The effect of varying magnitudes of whole-body vibration on several skeletal sites in mice. Ann. Biomed. Eng. 32:1149–1156, 2006.
Edwards, R. G., E. P. McCutcheon, and C. F. Knapp. Cardiovascular changes produced by brief whole-body vibration of animals. J. Appl. Physiol. 32:386–390, 1972.
Faith, R. F., and S. J. Miller. The need for sound and vibration standards in US research animal rooms. ALN Magazine July/August: 31–38, 2007.
Fritton, J. C., C. T. Rubin, Y. X. Qin, and K. J. McLeod. Whole-body vibration in the skeleton: development of a resonance-based testing device. Ann. Biomed. Eng. 25:831–839, 1997.
Gebresenbet, G., S. Aradom, F. S. Bulitta, and E. Hjerpe. Vibration levels and frequencies on vehicle and animals during transport. Biosyst. Eng. 110:10–19, 2011.
Griffin, M. J. Whole-body vibration and health. In: Handbook of Human Vibration, edited by M. J. Griffin. San Diego: Elsevier Academic Press, 1996, pp. 171–220.
Hill, P. S. M. Vibration and animal communication: a review. Integr. Comp. Biol. 41:1135–1142, 2001.
Judex, S., S. Boyd, Y.-X. Qin, S. Turner, K. Ye, R. Müller, and C. Rubin. Adaptations of trabecular bone to low magnitude vibrations result in more uniform stress and strain under load. J. Biomed. Eng. 31:12–20, 2003.
Judex, S., L.-R. Donahue, and C. Rubin. Genetic predisposition to low bone mass is paralleled by an enhanced sensitivity to signals anabolic to the skeleton. FASEB J. 16:1280–1282, 2002.
Li, Y., Y. Liu, Z. Jiang, J. Guan, G. Yi, S. Cheng, B. Yang, T. Fu, and Z. Wang. Behavioral changes related to Wenchuan devastating earthquake in mice. Bioelectromagnetics 30:613–620, 2009.
Ljunggren, F., J. Wang, and A. Agren. Human vibration perception from single- and dual-frequency components. J. Sound Vib. 300:13–24, 2007.
Lynch, M. A., M. D. Brodt, and M. J. Silva. Skeletal effects of whole-body vibration in adult and aged mice. J. Orthopaed. Res. 28:241–247, 2009.
McKeehen, J. N., S. A. Novotny, K. A. Baltgalvis, J. A. Call, D. J. Nuckley, and D. A. Lowe. Adaptations of mouse skeletal muscle to low-intensity vibration training. Med. Sci. Sports Exerc. 45:1051–1059, 2013.
Norton, J. N., W. L. Kinard, and R. P. Reynolds. Comparative vibration levels perceived among species in a laboratory animal facility. J. Am. Assoc. Lab. Anim. Sci. 50:653–659, 2011.
Parsons, K. C., and M. J. Griffin. Whole-body vibration perception threshold. J. Sound Vib. 121:237–258, 1988.
Perremans, S., J. M. Randall, G. Rombouts, E. Decuypere, and R. Geers. Effect of whole-body vibration in the vertical axis on cortisol and adrenocorticotropic hormone levels in piglets. J. Anim. Sci. 79:975–981, 2001.
Prisby, R. D., M.-H. Lafage-Proust, L. Malaval, A. Belli, and L. Vico. Effects of whole body vibration on the skeleton and other organ systems in man and animal models: what we know and what we need to know. Ageing Res. Rev. 7:319–329, 2008.
Randall, J. M., J. A. Duggan, M. A. Alami, and R. P. White. Frequency weighting for the aversion of broiler chickens to horizontal and vertical vibration. J. Agric. Eng. Res. 68:387–397, 1997a.
Randall, J. M., R. T. Mathews, and M. A. Stiles. Resonant frequencies of standing humans. Ergonomics 40:879–886, 1997.
Reynolds, R. P., W. L. Kinard, J. J. Degraff, N. Leverage, and J. N. Norton. Noise in a laboratory animal facility from the human and mouse perspectives. J. Am. Assoc. Lab. Anim. Sci. 49:592–597, 2010.
Romans, J. Effect of severe whole-body vibration on mice and methods of protection from vibration injury. WADC Techical Report 58-107, ASTIA Document No. AD 151070. Wright-Patterson Air Force Base (OH): Wright Air Development Centre, 1958.
Rubin, C. T., E. Capilla, Y. K. Luu, B. Busa, H. Crawford, D. J. Noland, V. Mittal, C. J. Rosen, J. E. Pessin, and S. Judex. Adipogenesis is inhibited by brief, daily exposure to high-frequency, extremely low-magnitude mechanical signals. Proc. Natl Acad. Sci. U.S.A. 104:17879–17884, 2007.
Rubin, C. T., D. W. Sommerfeldt, S. Judex, and Y.-X. Qin. Inhibition of osteopenia by low-magnitude, high-frequency mechanical stimuli. Drug Discov. Today 6:848–858, 2001.
Rubin, C. T., A. S. Turner, S. Bain, C. Mallinckrodt, and K. McLoed. Low mechanical signal strengthen long bones. Nature 412:603–604, 2001.
Rubin, C. T., A. S. Turner, R. Muller, E. Mittra, K. McLoed, W. Lin, and Y.-X. Qin. Quantity and quality of trabecular bone in the femur are enhanced by a strongly anabolic, noninvasive mechanical intervention. J. Bone Miner. Res. 17:349–357, 2002.
Sackler, A. M., and A. S. Weltman. Effects of vibration on the endocrine system of male and female rats. Aerospace Med. 37:158–166, 1966.
Sales, G. D., K. J. Wilson, K. E. Spencer, and S. R. Milligan. Environmental ultrasound in laboratories and animal houses: a possible cause for concern in the welfare and use of laboratory animals. Lab. Anim. 22:369–375, 1988.
Toraason, M. A., D. W. Badger, and G. L. Wright. Gastroinstestinal response in rats to vibration and restraint. Environ. Res. 23:341–347, 1980.
Ushakov, I. B., N. V. Soloshenko, and A. P. Koslovskij. The examination of resonance frequencies of vibration in rats. Kosm Biol Aviakosm Med. 17:65–68, 1983.
Ward, K., C. Alsop, J. Caulton, C. Rubin, J. Adams, and Z. Mughal. Low magnitude mechanical loading is osteogenic in children with disabling conditions. J. Bone Miner. Res. 19:360–369, 2004.
Wegner, K. H., J. D. Freeman, S. Fulzele, D. M. Immel, B. D. Powell, P. Molitor, Y. J. Chao, H.-S. Gao, M. Wlsalanty, M. W. Hamrick, C. M. Isales, and J. C. Yu. Effect of whole-body vibration on bone properties in aging mice. Bone 47:746–755, 2010.
Xie, L., J. M. Jacobson, E. S. Choi, B. Busa, L. R. Donahue, L. M. Miler, C. T. Rubin, and S. Judex. Low-level mechanical vibrations can influence bone resorption in the growing skeleton. Bone 39:1059–1066, 2006.
Xie, L., C. T. Rubin, and S. Judex. Enhancement of the adolescent murine musculoskeletal system using low-level mechanical vibrations. J. Appl. Physiol. 104:1056–1062, 2008.
Acknowledgments
We would like to thank Pramodh Ganapathy, Jozsef Bordas, and Drs. Roxanne Larsen and Charlotte Miller for their help and support throughout this project. This study was funded by the American Association of Laboratory Animal Science Grants for Laboratory Animal Science (GLAS) and the American College of Laboratory Animal Medicine Foundation Grants.
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Associate Editor Thurmon E. Lockhart oversaw the review of this article.
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Rabey, K.N., Li, Y., Norton, J.N. et al. Vibrating Frequency Thresholds in Mice and Rats: Implications for the Effects of Vibrations on Animal Health. Ann Biomed Eng 43, 1957–1964 (2015). https://doi.org/10.1007/s10439-014-1226-y
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DOI: https://doi.org/10.1007/s10439-014-1226-y