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Histologic findings of recipient corneas obtained via deep anterior lamellar keratoplasty

  • Clinical Investigation
  • Published:
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Abstract

Purpose

We sought to investigate the histologic findings of recipient corneas obtained via deep anterior lamellar keratoplasty (DALK).

Methods

The histology of three recipient corneas obtained from patients during DALK was investigated. In all cases, the Descemet membrane was successfully exposed without any perforation during surgery. The isolated corneal tissue was stained with hematoxylin-eosin and periodic acid-Schiff. In two cases, the tissues were examined using a transmission electron microscope.

Results

In one cornea obtained via DALK, only the corneal stroma was observed, and the Descemet membrane was not confirmed. In another case, the recipient cornea was detached within the Descemet membrane. In the third cornea, the banded layer membrane was partially confirmed.

Conclusions

These findings suggest that the recipient corneas separated at different layers during the DALK procedure. With our surgical technique, the detachment of the Descemet membrane may occur at a mechanically weak segment. This separation site may not be between the Descemet membrane and the corneal stroma.

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Conflicts of interest

S. Ishimaru, None; T. Senoo, None; N. Gotoh, None; A. Kurosu, None; K. Chiba, None.

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Authors and Affiliations

  1. Department of Ophthalmology, Dokkyo Medical University School of Medicine, 880 Mibu, Shimotsuga, Tochigi, 321-0293, Japan

    Shimpei Ishimaru, Tadashi Senoo, Norihito Gotoh & Keizo Chiba

  2. Department of Legal Medicine, Dokkyo Medical University School of Medicine, Tochigi, Japan

    Akira Kurosu

Authors
  1. Shimpei Ishimaru
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  2. Tadashi Senoo
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  3. Norihito Gotoh
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  4. Akira Kurosu
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  5. Keizo Chiba
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Corresponding author

Correspondence to Tadashi Senoo.

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Ishimaru, S., Senoo, T., Gotoh, N. et al. Histologic findings of recipient corneas obtained via deep anterior lamellar keratoplasty. Jpn J Ophthalmol 58, 342–347 (2014). https://doi.org/10.1007/s10384-014-0317-5

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  • DOI: https://doi.org/10.1007/s10384-014-0317-5

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