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Concurrent Ex vitro Rooting and Hardening in Ney Poovan Banana (Musa AB): Effect of Carbon Sources and their Concentrations

Gleichzeitige ex vitro Bewurzelung und Abhärtung bei der Bananensorte Ney Poovan (Musa AB): Auswirkung von Kohlenstoff-Quellen in unterschiedlichen Konzentrationen

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Abstract

Mass multiplication of non-Cavendish group of bananas has been limited due to non availability of reliable multiplication protocol and higher cost of production. The process of root induction and hardening are considered to be the most capital and labour intensive activities in micropropagation of any species. Carbon sources being one of the prime components governing the ex vitro survival of the plants need to be studied to maximize the success rate. Present is an attempt to study the effect of kind and concentration of carbon source on concurrent ex vitro rooting and hardening (CEVRH) in Ney Poovan banana. The results revealed that kind and concentration of carbon source present in the nutrient pool at the time of multiplication had a significant effect on the ex vitro performance of the plantlets. Better ex vitro rooting and hardening performance was exhibited by the shoots obtained from glucose (2 %) supplemented medium. Though rooting percentage was reduced in this treatment, considering the superior performance of the plantlets, it could be recommended to the commercial units for large-scale propagation of Ney Poovan banana plants. The concept of simultaneous rooting and hardening could be a boon for the varieties showing poor multiplication rate resulting in their higher cost of production.

Zusammenfassung

Die Massenvermehrung der Bananen, die nicht zu der Cavendish-Gruppe gehören, wird durch das Fehlen einer verlässlichen Vermehrungsanleitung und höheren Produktionskosten begrenzt. Die Vorgänge der Bewurzelungsinduktion und der Abhärtung werden bei allen Spezies als die zentralen und arbeitsintensivsten Tätigkeiten in der Mikrovermehrung angesehen. Als eine der wesentlichen Komponenten, die die Überlebensrate der Pflanzen ex vitro beeinflusst, muss die Kohlenstoff-Quelle untersucht werden, um die Erfolgsrate zu maximieren. In dem vorliegenden Beitrag handelt es sich um den Versuch, den Einfluss von Art und Konzentration der Kohlenstoff-Quelle auf die gleichzeitige ex vitro Bewurzelung und Abhärtung (CEVRH) bei der Sorte Ney Poovan zu untersuchen. Die Ergebnisse zeigen, dass die Art und Konzentration der Kohlenstoff-Quelle, die sich zum Zeitpunkt der Multiplikation im Nährmedium befinden, einen signifikanten Einfluss auf die ex vitro Entwicklung der Setzlinge hat. Eine bessere Bewurzelung ex vitro und ein besseres Abhärtungsergebnis wurden von den Pflanzensprossen gezeigt, die von einem, mit Glukose (2 %) angereicherten, Nährmedium stammten. Obwohl die Bewurzelungsrate in dieser Behandlung reduziert war, könnte diese Variante in Anbetracht der besseren Leistungsfähigkeit der Setzlinge für die gewerblichen Betriebe zur Massenvermehrung der Bananensorte Ney Poovan empfohlen werden. Die Idee der gleichzeitigen Bewurzelung und Abhärtung könnte sich als die geeignete Methode bei den Sorten herausstellen, die eine schlechte Vermehrungsrate verbunden mit höheren Produktionskosten aufweisen.

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Acknowledgements

P. B. is thankful to the Department of Science and Technology, Government of India for providing financial assistance in the form of an INSPIRE fellowship (IF 10077).

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Authors and Affiliations

  1. Division of Horticulture, University of Agricultural Sciences, GKVK Campus, 560065, Bengaluru, India

    P. Bohra, A. A. Waman & B. N. Sathyanarayana

  2. P.G. Centre, University of Horticultural Sciences (Bagalkot), GKVK Campus, 560065, Bengaluru, India

    K. Umesha

  3. Plant Tissue Culture Laboratory, Division of Horticulture and Forestry, ICAR – Central Island Agricultural Research Institute, 744 101, Port Blair, India

    P. Bohra & A. A. Waman

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  1. P. Bohra
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  2. A. A. Waman
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Correspondence to P. Bohra.

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Bohra, P., Waman, A.A., Sathyanarayana, B.N. et al. Concurrent Ex vitro Rooting and Hardening in Ney Poovan Banana (Musa AB): Effect of Carbon Sources and their Concentrations. Erwerbs-Obstbau 58, 193–198 (2016). https://doi.org/10.1007/s10341-016-0271-0

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